Probing for a ribosome code in Escherichia coli using integration host factor
Integration host factor (IHF) is an important DNA binding and bending protein which regulates expression of a large number of genes in Escherichia coli. Our experimental results show that a polyhistidine-tagged single-chain IHF protein, sc1HF2, has the ability to interact with E. coli ribosomes in a...
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| Format: | Thesis |
| Language: | English |
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Nanyang Technological University
2014
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| Online Access: | http://hdl.handle.net/10356/59963 |
| _version_ | 1811684918465396736 |
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| author | Cai, Hui |
| author2 | Peter Droge |
| author_facet | Peter Droge Cai, Hui |
| author_sort | Cai, Hui |
| collection | NTU |
| description | Integration host factor (IHF) is an important DNA binding and bending protein which regulates expression of a large number of genes in Escherichia coli. Our experimental results show that a polyhistidine-tagged single-chain IHF protein, sc1HF2, has the ability to interact with E. coli ribosomes in a stable manner. To date, the interaction between IHF and ribosome has not been studied. Using sclHF2 we were able to purify ribosomes incorporated with sclHF2 and further confirmed the interaction takes place on the 50S ribosomal subunit. Moreover, we found correlations between IHF expression and bacterial survival mechanisms in times of starvation, especially ribosome
integrity. With the help of mass-spectrometry-based protein identification, we identified several binding partners exclusive for IHF-bound ribosomes. These findings suggest new roles for IHF in protein transport, stationary phase, ribosome metabolism and gene regulation. |
| first_indexed | 2024-10-01T04:36:16Z |
| format | Thesis |
| id | ntu-10356/59963 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:36:16Z |
| publishDate | 2014 |
| publisher | Nanyang Technological University |
| record_format | dspace |
| spelling | ntu-10356/599632023-02-28T18:37:55Z Probing for a ribosome code in Escherichia coli using integration host factor Cai, Hui Peter Droge School of Biological Sciences PDroge@ntu.edu.sg Science::Biological sciences Integration host factor (IHF) is an important DNA binding and bending protein which regulates expression of a large number of genes in Escherichia coli. Our experimental results show that a polyhistidine-tagged single-chain IHF protein, sc1HF2, has the ability to interact with E. coli ribosomes in a stable manner. To date, the interaction between IHF and ribosome has not been studied. Using sclHF2 we were able to purify ribosomes incorporated with sclHF2 and further confirmed the interaction takes place on the 50S ribosomal subunit. Moreover, we found correlations between IHF expression and bacterial survival mechanisms in times of starvation, especially ribosome integrity. With the help of mass-spectrometry-based protein identification, we identified several binding partners exclusive for IHF-bound ribosomes. These findings suggest new roles for IHF in protein transport, stationary phase, ribosome metabolism and gene regulation. Master of Science 2014-05-21T03:35:02Z 2014-05-21T03:35:02Z 2013 2013 Thesis http://hdl.handle.net/10356/59963 en This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0). 84 p. application/pdf Nanyang Technological University |
| spellingShingle | Science::Biological sciences Cai, Hui Probing for a ribosome code in Escherichia coli using integration host factor |
| title | Probing for a ribosome code in Escherichia coli using integration host factor |
| title_full | Probing for a ribosome code in Escherichia coli using integration host factor |
| title_fullStr | Probing for a ribosome code in Escherichia coli using integration host factor |
| title_full_unstemmed | Probing for a ribosome code in Escherichia coli using integration host factor |
| title_short | Probing for a ribosome code in Escherichia coli using integration host factor |
| title_sort | probing for a ribosome code in escherichia coli using integration host factor |
| topic | Science::Biological sciences |
| url | http://hdl.handle.net/10356/59963 |
| work_keys_str_mv | AT caihui probingforaribosomecodeinescherichiacoliusingintegrationhostfactor |