Synthesis of functionalized polymer nano-particles as fluorescent probes for bio-imaging
The objective was to characterise and optimise the desolvation process of Bovine Serum Albumin (BSA) for the preparation of nano-particles for bio-imaging applications. Following on the desolvation process of protein, the resulting nano-particles were stabilised by the addition of varying amounts of...
| Main Author: | |
|---|---|
| Other Authors: | |
| Format: | Final Year Project (FYP) |
| Language: | English |
| Published: |
2014
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/10356/60178 |
| _version_ | 1811695280495525888 |
|---|---|
| author | Tan, Kian Meng |
| author2 | School of Electrical and Electronic Engineering |
| author_facet | School of Electrical and Electronic Engineering Tan, Kian Meng |
| author_sort | Tan, Kian Meng |
| collection | NTU |
| description | The objective was to characterise and optimise the desolvation process of Bovine Serum Albumin (BSA) for the preparation of nano-particles for bio-imaging applications. Following on the desolvation process of protein, the resulting nano-particles were stabilised by the addition of varying amounts of glutaraldehyde(cross-linker).
This report directed towards the synthesis of functionalized polymer nanoparticles as fluorescent probes for bio-imaging. Hence, number of method on the synthesis of fluorescence loaded BSA NPs were carried out.
Synthesis of fluorescence loaded BSA NPs were carried out using the 3 step process, sonication, cross-linking and THF removal. Other parameter including reducing particle size, stirring process, using ethanol as a desolvating agent, abosorption and emission analysis were also carried out.
The result has shown that using THF as a desolvating agent produce a particle size of less than 200nm. The result obtained implies that the brightness of the fluorescent-doped NPs is proportional to the number of encapsulated dye molecules. |
| first_indexed | 2024-10-01T07:20:58Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/60178 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T07:20:58Z |
| publishDate | 2014 |
| record_format | dspace |
| spelling | ntu-10356/601782023-07-07T16:03:45Z Synthesis of functionalized polymer nano-particles as fluorescent probes for bio-imaging Tan, Kian Meng School of Electrical and Electronic Engineering Yong Ken Tye DRNTU::Science The objective was to characterise and optimise the desolvation process of Bovine Serum Albumin (BSA) for the preparation of nano-particles for bio-imaging applications. Following on the desolvation process of protein, the resulting nano-particles were stabilised by the addition of varying amounts of glutaraldehyde(cross-linker). This report directed towards the synthesis of functionalized polymer nanoparticles as fluorescent probes for bio-imaging. Hence, number of method on the synthesis of fluorescence loaded BSA NPs were carried out. Synthesis of fluorescence loaded BSA NPs were carried out using the 3 step process, sonication, cross-linking and THF removal. Other parameter including reducing particle size, stirring process, using ethanol as a desolvating agent, abosorption and emission analysis were also carried out. The result has shown that using THF as a desolvating agent produce a particle size of less than 200nm. The result obtained implies that the brightness of the fluorescent-doped NPs is proportional to the number of encapsulated dye molecules. Bachelor of Engineering 2014-05-23T02:21:29Z 2014-05-23T02:21:29Z 2014 2014 Final Year Project (FYP) http://hdl.handle.net/10356/60178 en Nanyang Technological University 65 p. application/pdf |
| spellingShingle | DRNTU::Science Tan, Kian Meng Synthesis of functionalized polymer nano-particles as fluorescent probes for bio-imaging |
| title | Synthesis of functionalized polymer nano-particles as fluorescent probes for bio-imaging |
| title_full | Synthesis of functionalized polymer nano-particles as fluorescent probes for bio-imaging |
| title_fullStr | Synthesis of functionalized polymer nano-particles as fluorescent probes for bio-imaging |
| title_full_unstemmed | Synthesis of functionalized polymer nano-particles as fluorescent probes for bio-imaging |
| title_short | Synthesis of functionalized polymer nano-particles as fluorescent probes for bio-imaging |
| title_sort | synthesis of functionalized polymer nano particles as fluorescent probes for bio imaging |
| topic | DRNTU::Science |
| url | http://hdl.handle.net/10356/60178 |
| work_keys_str_mv | AT tankianmeng synthesisoffunctionalizedpolymernanoparticlesasfluorescentprobesforbioimaging |