Effect of plant extract on the preservation of organ/tissue : an investigation on cell viability and mechanical properties
In this study, Aloe Vera extract has been explored as a pharmacologic additive in tissue preservation medium to prolong the “safe” ischemia time. Aortic (AV) and pulmonary (PV) valve leaflets were harvested from freshly procured porcine hearts and then subjected to preservation treatment of differen...
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| Format: | Thesis |
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2008
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| Online Access: | http://hdl.handle.net/10356/6018 |
| _version_ | 1826127229360275456 |
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| author | Bai, Lin |
| author2 | Chong, Chuh Khiun |
| author_facet | Chong, Chuh Khiun Bai, Lin |
| author_sort | Bai, Lin |
| collection | NTU |
| description | In this study, Aloe Vera extract has been explored as a pharmacologic additive in tissue preservation medium to prolong the “safe” ischemia time. Aortic (AV) and pulmonary (PV) valve leaflets were harvested from freshly procured porcine hearts and then subjected to preservation treatment of different Aloe Vera extract concentrations and ischemic time of 0, 18, and 60 hours. Uniaxial mechanical test and MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) assay were performed to assess the mechanical properties and cell viability, respectively. The result showed that the preservation time (P<0.05) has a significant effect on the Failure Strain of AV and the Failure Load of PV, as well as the OD of PV. |
| first_indexed | 2024-10-01T07:05:23Z |
| format | Thesis |
| id | ntu-10356/6018 |
| institution | Nanyang Technological University |
| last_indexed | 2024-10-01T07:05:23Z |
| publishDate | 2008 |
| record_format | dspace |
| spelling | ntu-10356/60182023-03-11T17:01:56Z Effect of plant extract on the preservation of organ/tissue : an investigation on cell viability and mechanical properties Bai, Lin Chong, Chuh Khiun School of Mechanical and Aerospace Engineering DRNTU::Engineering::Bioengineering In this study, Aloe Vera extract has been explored as a pharmacologic additive in tissue preservation medium to prolong the “safe” ischemia time. Aortic (AV) and pulmonary (PV) valve leaflets were harvested from freshly procured porcine hearts and then subjected to preservation treatment of different Aloe Vera extract concentrations and ischemic time of 0, 18, and 60 hours. Uniaxial mechanical test and MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) assay were performed to assess the mechanical properties and cell viability, respectively. The result showed that the preservation time (P<0.05) has a significant effect on the Failure Strain of AV and the Failure Load of PV, as well as the OD of PV. Master of Science (Biomedical Engineering) 2008-09-17T11:04:52Z 2008-09-17T11:04:52Z 2005 2005 Thesis http://hdl.handle.net/10356/6018 Nanyang Technological University application/pdf |
| spellingShingle | DRNTU::Engineering::Bioengineering Bai, Lin Effect of plant extract on the preservation of organ/tissue : an investigation on cell viability and mechanical properties |
| title | Effect of plant extract on the preservation of organ/tissue : an investigation on cell viability and mechanical properties |
| title_full | Effect of plant extract on the preservation of organ/tissue : an investigation on cell viability and mechanical properties |
| title_fullStr | Effect of plant extract on the preservation of organ/tissue : an investigation on cell viability and mechanical properties |
| title_full_unstemmed | Effect of plant extract on the preservation of organ/tissue : an investigation on cell viability and mechanical properties |
| title_short | Effect of plant extract on the preservation of organ/tissue : an investigation on cell viability and mechanical properties |
| title_sort | effect of plant extract on the preservation of organ tissue an investigation on cell viability and mechanical properties |
| topic | DRNTU::Engineering::Bioengineering |
| url | http://hdl.handle.net/10356/6018 |
| work_keys_str_mv | AT bailin effectofplantextractonthepreservationoforgantissueaninvestigationoncellviabilityandmechanicalproperties |