Activation and regulation of IL-1β family of cytokines by EV71 infection
Severe cases of hand, foot and mouth disease (HFMD) are usually caused by Enterovirus 71 (EV71) and linked to elevated levels of cytokines. In this study, we first tested the ability of EV71 (BrCr and WD strain) in infecting differentiated monocyte lymphoma (U937) cells, epithelial lung cancer (A549...
| Main Author: | |
|---|---|
| Other Authors: | |
| Format: | Final Year Project (FYP) |
| Language: | English |
| Published: |
2014
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/10356/60262 |
| _version_ | 1826115010925953024 |
|---|---|
| author | Aw, Ashley Jong Ghut |
| author2 | Liu Ding Xiang |
| author_facet | Liu Ding Xiang Aw, Ashley Jong Ghut |
| author_sort | Aw, Ashley Jong Ghut |
| collection | NTU |
| description | Severe cases of hand, foot and mouth disease (HFMD) are usually caused by Enterovirus 71 (EV71) and linked to elevated levels of cytokines. In this study, we first tested the ability of EV71 (BrCr and WD strain) in infecting differentiated monocyte lymphoma (U937) cells, epithelial lung cancer (A549) cells and liver cancer (HUH7) cells. We found that both EV71 strains only infected A549 and HUH7 cells. While EV71 could not infect differentiated U937 cells, the transfection of purified viral RNA into these cells enabled viral replication. We next measured the levels of cytokines IL-1β, IL-8 and IL-18 at different time points after EV71 infection or transfection. We detected (i) IL-1β in EV71 transfected differentiated U937 cells, (ii) IL-18 in EV71 transfected differentiated U937 cells and infected HUH7 cells, and (iii) IL-8 in all three infected/transfected cell types. Additionally, we found upregulation of IL-1β and IL-18 levels in these cells, correlating with viral mRNA levels. While IL-8 upregulation in infected A549 and HUH7 cells correlate with viral mRNA levels, it showed negative correlation with viral mRNA level in transfected differentiated U937 cells. We anticipate that the study of cytokine kinetics in EV71 infected cells would facilitate the understanding of EV71 pathogenesis. |
| first_indexed | 2024-10-01T03:48:32Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/60262 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:48:32Z |
| publishDate | 2014 |
| record_format | dspace |
| spelling | ntu-10356/602622019-12-10T10:49:05Z Activation and regulation of IL-1β family of cytokines by EV71 infection Aw, Ashley Jong Ghut Liu Ding Xiang School of Biological Sciences Huang Mei DRNTU::Science::Biological sciences::Microbiology::Virology DRNTU::Science::Biological sciences::Microbiology::Immunology Severe cases of hand, foot and mouth disease (HFMD) are usually caused by Enterovirus 71 (EV71) and linked to elevated levels of cytokines. In this study, we first tested the ability of EV71 (BrCr and WD strain) in infecting differentiated monocyte lymphoma (U937) cells, epithelial lung cancer (A549) cells and liver cancer (HUH7) cells. We found that both EV71 strains only infected A549 and HUH7 cells. While EV71 could not infect differentiated U937 cells, the transfection of purified viral RNA into these cells enabled viral replication. We next measured the levels of cytokines IL-1β, IL-8 and IL-18 at different time points after EV71 infection or transfection. We detected (i) IL-1β in EV71 transfected differentiated U937 cells, (ii) IL-18 in EV71 transfected differentiated U937 cells and infected HUH7 cells, and (iii) IL-8 in all three infected/transfected cell types. Additionally, we found upregulation of IL-1β and IL-18 levels in these cells, correlating with viral mRNA levels. While IL-8 upregulation in infected A549 and HUH7 cells correlate with viral mRNA levels, it showed negative correlation with viral mRNA level in transfected differentiated U937 cells. We anticipate that the study of cytokine kinetics in EV71 infected cells would facilitate the understanding of EV71 pathogenesis. Bachelor of Science in Biological Sciences 2014-05-26T04:21:46Z 2014-05-26T04:21:46Z 2014 2014 Final Year Project (FYP) http://hdl.handle.net/10356/60262 en Nanyang Technological University 27 p. application/msword |
| spellingShingle | DRNTU::Science::Biological sciences::Microbiology::Virology DRNTU::Science::Biological sciences::Microbiology::Immunology Aw, Ashley Jong Ghut Activation and regulation of IL-1β family of cytokines by EV71 infection |
| title | Activation and regulation of IL-1β family of cytokines by EV71 infection |
| title_full | Activation and regulation of IL-1β family of cytokines by EV71 infection |
| title_fullStr | Activation and regulation of IL-1β family of cytokines by EV71 infection |
| title_full_unstemmed | Activation and regulation of IL-1β family of cytokines by EV71 infection |
| title_short | Activation and regulation of IL-1β family of cytokines by EV71 infection |
| title_sort | activation and regulation of il 1β family of cytokines by ev71 infection |
| topic | DRNTU::Science::Biological sciences::Microbiology::Virology DRNTU::Science::Biological sciences::Microbiology::Immunology |
| url | http://hdl.handle.net/10356/60262 |
| work_keys_str_mv | AT awashleyjongghut activationandregulationofil1bfamilyofcytokinesbyev71infection |