PCR amplification of single cell isolation via droplet-based microfluidics
Conventional DNA profiling work involved using a large amount of white blood cells obtained from the crime scene blood sample without quantitation. Therefore, introducing the concept of droplet-based microfluidics to perform single cell encapsulation serves as a potential methodology for DNA quantit...
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| Other Authors: | |
| Format: | Final Year Project (FYP) |
| Language: | English |
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2014
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| Online Access: | http://hdl.handle.net/10356/60805 |
| _version_ | 1824455289782403072 |
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| author | Hong, Fion Li Ying |
| author2 | Kang Yue Jun |
| author_facet | Kang Yue Jun Hong, Fion Li Ying |
| author_sort | Hong, Fion Li Ying |
| collection | NTU |
| description | Conventional DNA profiling work involved using a large amount of white blood cells obtained from the crime scene blood sample without quantitation. Therefore, introducing the concept of droplet-based microfluidics to perform single cell encapsulation serves as a potential methodology for DNA quantitation in forensic science applications.
The project involved fabricating a lab-on-a-chip device for single cell encapsulation through complete soft lithography process. The cells were stained and visualized under the fluorescence microscope to observe and detect single cell encapsulated within individual droplet. The key factors to achieving the objectives of this study were optimization of oil phase composition, flow rates of both oil and water phases, channel dimensions, and cell densities.
These techniques investigated above will lay grounds in developing a droplet-based microfluidic lab chip that is capable of performing single cell isolation for DNA quantitation. By developing a single cell analytical instrument for DNA profiling with the integration of cell lysis and PCR, this would establish a promising foothold for future work in the forensic science field. |
| first_indexed | 2025-02-19T03:35:51Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/60805 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2025-02-19T03:35:51Z |
| publishDate | 2014 |
| record_format | dspace |
| spelling | ntu-10356/608052023-03-03T15:40:51Z PCR amplification of single cell isolation via droplet-based microfluidics Hong, Fion Li Ying Kang Yue Jun School of Chemical and Biomedical Engineering DRNTU::Engineering Conventional DNA profiling work involved using a large amount of white blood cells obtained from the crime scene blood sample without quantitation. Therefore, introducing the concept of droplet-based microfluidics to perform single cell encapsulation serves as a potential methodology for DNA quantitation in forensic science applications. The project involved fabricating a lab-on-a-chip device for single cell encapsulation through complete soft lithography process. The cells were stained and visualized under the fluorescence microscope to observe and detect single cell encapsulated within individual droplet. The key factors to achieving the objectives of this study were optimization of oil phase composition, flow rates of both oil and water phases, channel dimensions, and cell densities. These techniques investigated above will lay grounds in developing a droplet-based microfluidic lab chip that is capable of performing single cell isolation for DNA quantitation. By developing a single cell analytical instrument for DNA profiling with the integration of cell lysis and PCR, this would establish a promising foothold for future work in the forensic science field. Bachelor of Engineering (Chemical and Biomolecular Engineering) 2014-05-30T07:38:37Z 2014-05-30T07:38:37Z 2014 2014 Final Year Project (FYP) http://hdl.handle.net/10356/60805 en Nanyang Technological University 58 p. application/pdf |
| spellingShingle | DRNTU::Engineering Hong, Fion Li Ying PCR amplification of single cell isolation via droplet-based microfluidics |
| title | PCR amplification of single cell isolation via droplet-based microfluidics |
| title_full | PCR amplification of single cell isolation via droplet-based microfluidics |
| title_fullStr | PCR amplification of single cell isolation via droplet-based microfluidics |
| title_full_unstemmed | PCR amplification of single cell isolation via droplet-based microfluidics |
| title_short | PCR amplification of single cell isolation via droplet-based microfluidics |
| title_sort | pcr amplification of single cell isolation via droplet based microfluidics |
| topic | DRNTU::Engineering |
| url | http://hdl.handle.net/10356/60805 |
| work_keys_str_mv | AT hongfionliying pcramplificationofsinglecellisolationviadropletbasedmicrofluidics |