Engineering artificial small RNAs for conditional gene silencing in escherichia coli
Conditional gene silencing is a promising technology within the microbiology field. Silencing a gene from a bacteria or cell will greatly change the cell function and morphology. This can be achieved using RNA interference technologies such as small regulatory RNAs (sRNA). However, the specificity o...
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| Format: | Final Year Project (FYP) |
| Language: | English |
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2014
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| Online Access: | http://hdl.handle.net/10356/60856 |
| _version_ | 1826128453354651648 |
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| author | Tan, Tong Wei |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Tan, Tong Wei |
| author_sort | Tan, Tong Wei |
| collection | NTU |
| description | Conditional gene silencing is a promising technology within the microbiology field. Silencing a gene from a bacteria or cell will greatly change the cell function and morphology. This can be achieved using RNA interference technologies such as small regulatory RNAs (sRNA). However, the specificity of sRNA remains a huge challenge in effective adoption of sRNA in conditional gene silencing as it may have huge amounts of off target activity. Here I discuss the potential application of conditional gene silencing in modulating the death pathways of Escherichia coli. By attenuating the death pathways within E. coli, there could potentially improve cell viability. |
| first_indexed | 2024-10-01T07:25:13Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/60856 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T07:25:13Z |
| publishDate | 2014 |
| record_format | dspace |
| spelling | ntu-10356/608562023-03-03T15:39:06Z Engineering artificial small RNAs for conditional gene silencing in escherichia coli Tan, Tong Wei School of Chemical and Biomedical Engineering Song Hao DRNTU::Engineering Conditional gene silencing is a promising technology within the microbiology field. Silencing a gene from a bacteria or cell will greatly change the cell function and morphology. This can be achieved using RNA interference technologies such as small regulatory RNAs (sRNA). However, the specificity of sRNA remains a huge challenge in effective adoption of sRNA in conditional gene silencing as it may have huge amounts of off target activity. Here I discuss the potential application of conditional gene silencing in modulating the death pathways of Escherichia coli. By attenuating the death pathways within E. coli, there could potentially improve cell viability. Bachelor of Engineering (Chemical and Biomolecular Engineering) 2014-06-02T03:44:28Z 2014-06-02T03:44:28Z 2014 2014 Final Year Project (FYP) http://hdl.handle.net/10356/60856 en Nanyang Technological University 28 p. application/pdf |
| spellingShingle | DRNTU::Engineering Tan, Tong Wei Engineering artificial small RNAs for conditional gene silencing in escherichia coli |
| title | Engineering artificial small RNAs for conditional gene silencing in escherichia coli |
| title_full | Engineering artificial small RNAs for conditional gene silencing in escherichia coli |
| title_fullStr | Engineering artificial small RNAs for conditional gene silencing in escherichia coli |
| title_full_unstemmed | Engineering artificial small RNAs for conditional gene silencing in escherichia coli |
| title_short | Engineering artificial small RNAs for conditional gene silencing in escherichia coli |
| title_sort | engineering artificial small rnas for conditional gene silencing in escherichia coli |
| topic | DRNTU::Engineering |
| url | http://hdl.handle.net/10356/60856 |
| work_keys_str_mv | AT tantongwei engineeringartificialsmallrnasforconditionalgenesilencinginescherichiacoli |