The influence of different matrix proteins on cell traction forces and differentiation
Extracellular matrix (ECM) is the extracellular component of animal tissue which plays a significant role not only to provide structural support but also stimulates various biological functions. Extracellular matrix proteins enable the dictation of cellular behavior in terms of proliferation, adhesi...
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| Format: | Final Year Project (FYP) |
| Language: | English |
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2014
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| Online Access: | http://hdl.handle.net/10356/61579 |
| _version_ | 1811695487105892352 |
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| author | Ng, Shao Ting |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Ng, Shao Ting |
| author_sort | Ng, Shao Ting |
| collection | NTU |
| description | Extracellular matrix (ECM) is the extracellular component of animal tissue which plays a significant role not only to provide structural support but also stimulates various biological functions. Extracellular matrix proteins enable the dictation of cellular behavior in terms of proliferation, adhesion, migration or differentiation. Hence, it is an important factor in the reproduction of cells biological niches in vitro, which guides stem cells to differentiate into different tissue lineage. This study was done with the aim of determining their efficacy in encouraging cell growth and differentiation towards fibrochondrogenic lineage. Four ECM proteins namely fibronectin, collagen type I, gelatin and chondroitin sulfate were coated onto TCP plates where the hMSCs were seeded and subjected to complete DMEM supplemented with ascorbic acid for 2 weeks, followed by another 4 weeks of chondrogenic differentiation. The cells adhesion, spreading, proliferation, traction forces and their fibrochondrogenic markers were evaluated at different time-points. hMSCs cultured on collagen type I, fibronectin and gelatin coated-surfaces resulted in significant higher cell proliferation rate compared to the control (tissue culture polystyrene) at the end of 2 weeks. Besides that, the regulation of fibrochondrogenic markers varies differently between hMSCs grown on different matrix protein. For cell traction forces, Col1 surface was found to provide a more favorable environment for cell-cell and cell-matrix interactions throughout the 7 days of analysis. This study shows the influence of different ECM proteins on stem cells’ fate, and provides a platform for application of these matrix proteins in optimizing fibrochondrogenic tissues development within a scaffold. |
| first_indexed | 2024-10-01T07:24:15Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/61579 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T07:24:15Z |
| publishDate | 2014 |
| record_format | dspace |
| spelling | ntu-10356/615792023-03-03T15:39:05Z The influence of different matrix proteins on cell traction forces and differentiation Ng, Shao Ting School of Chemical and Biomedical Engineering Kang YueJun DRNTU::Engineering::Bioengineering Extracellular matrix (ECM) is the extracellular component of animal tissue which plays a significant role not only to provide structural support but also stimulates various biological functions. Extracellular matrix proteins enable the dictation of cellular behavior in terms of proliferation, adhesion, migration or differentiation. Hence, it is an important factor in the reproduction of cells biological niches in vitro, which guides stem cells to differentiate into different tissue lineage. This study was done with the aim of determining their efficacy in encouraging cell growth and differentiation towards fibrochondrogenic lineage. Four ECM proteins namely fibronectin, collagen type I, gelatin and chondroitin sulfate were coated onto TCP plates where the hMSCs were seeded and subjected to complete DMEM supplemented with ascorbic acid for 2 weeks, followed by another 4 weeks of chondrogenic differentiation. The cells adhesion, spreading, proliferation, traction forces and their fibrochondrogenic markers were evaluated at different time-points. hMSCs cultured on collagen type I, fibronectin and gelatin coated-surfaces resulted in significant higher cell proliferation rate compared to the control (tissue culture polystyrene) at the end of 2 weeks. Besides that, the regulation of fibrochondrogenic markers varies differently between hMSCs grown on different matrix protein. For cell traction forces, Col1 surface was found to provide a more favorable environment for cell-cell and cell-matrix interactions throughout the 7 days of analysis. This study shows the influence of different ECM proteins on stem cells’ fate, and provides a platform for application of these matrix proteins in optimizing fibrochondrogenic tissues development within a scaffold. Bachelor of Engineering (Chemical and Biomolecular Engineering) 2014-06-16T03:39:39Z 2014-06-16T03:39:39Z 2014 2014 Final Year Project (FYP) http://hdl.handle.net/10356/61579 en Nanyang Technological University 61 p. application/pdf |
| spellingShingle | DRNTU::Engineering::Bioengineering Ng, Shao Ting The influence of different matrix proteins on cell traction forces and differentiation |
| title | The influence of different matrix proteins on cell traction forces and differentiation |
| title_full | The influence of different matrix proteins on cell traction forces and differentiation |
| title_fullStr | The influence of different matrix proteins on cell traction forces and differentiation |
| title_full_unstemmed | The influence of different matrix proteins on cell traction forces and differentiation |
| title_short | The influence of different matrix proteins on cell traction forces and differentiation |
| title_sort | influence of different matrix proteins on cell traction forces and differentiation |
| topic | DRNTU::Engineering::Bioengineering |
| url | http://hdl.handle.net/10356/61579 |
| work_keys_str_mv | AT ngshaoting theinfluenceofdifferentmatrixproteinsoncelltractionforcesanddifferentiation AT ngshaoting influenceofdifferentmatrixproteinsoncelltractionforcesanddifferentiation |