Engineering synthetic promoters as dynamic controllers in saccharomyces cerevisiae
Metabolic engineering of yeast is an attractive way to produce advanced biofuels. However, engineering of yeast by introducing heterologous proteins and pathways can reduce growth rates and impact productivity. Towards optimizing yeast strains, sensor-regulators can assist the optimal usage of cellu...
| Main Author: | |
|---|---|
| Other Authors: | |
| Format: | Thesis |
| Language: | English |
| Published: |
2014
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/61754 |
| _version_ | 1811680957553442816 |
|---|---|
| author | Teo, Wei Suong |
| author2 | Chang Wook, Matthew |
| author_facet | Chang Wook, Matthew Teo, Wei Suong |
| author_sort | Teo, Wei Suong |
| collection | NTU |
| description | Metabolic engineering of yeast is an attractive way to produce advanced biofuels. However, engineering of yeast by introducing heterologous proteins and pathways can reduce growth rates and impact productivity. Towards optimizing yeast strains, sensor-regulators can assist the optimal usage of cellular resources, where protein expression can be regulated by the concentration of important metabolites. In this thesis, synthetic promoters were engineered and heterologous transcriptional repressors were expressed in order to create dynamic controllers in yeast. First, fatty acids/fatty acyl-CoA sensor-regulators were made as they are key intermediates in the production of fatty acid derived biofuels, which are suitable for direct use in current transportation infrastructure. This enables fatty acid dependant control of fatty acid derivative producing proteins. Second, AND-gate dynamic controllers that combine inducible promoter function, which enables cells to quickly accumulate biomass before triggering the production of biofuel producing proteins, and fatty acid sensing-regulation were constructed. Third, xylose sensor-regulators were created, where xylose is a major sugar component in the affordable lignocellulose biomass carbon source. This allows regulation of xylose utilizing proteins based on the amount of xylose sugars detected. The function and performance of these synthetic promoters to dynamically control yEGFP reporter protein were demonstrated. |
| first_indexed | 2024-10-01T03:33:18Z |
| format | Thesis |
| id | ntu-10356/61754 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:33:18Z |
| publishDate | 2014 |
| record_format | dspace |
| spelling | ntu-10356/617542023-03-03T15:59:11Z Engineering synthetic promoters as dynamic controllers in saccharomyces cerevisiae Teo, Wei Suong Chang Wook, Matthew Kunn Hadinoto Ong School of Chemical and Biomedical Engineering DRNTU::Engineering::Chemical engineering::Biotechnology DRNTU::Engineering::Chemical engineering::Biochemical engineering Metabolic engineering of yeast is an attractive way to produce advanced biofuels. However, engineering of yeast by introducing heterologous proteins and pathways can reduce growth rates and impact productivity. Towards optimizing yeast strains, sensor-regulators can assist the optimal usage of cellular resources, where protein expression can be regulated by the concentration of important metabolites. In this thesis, synthetic promoters were engineered and heterologous transcriptional repressors were expressed in order to create dynamic controllers in yeast. First, fatty acids/fatty acyl-CoA sensor-regulators were made as they are key intermediates in the production of fatty acid derived biofuels, which are suitable for direct use in current transportation infrastructure. This enables fatty acid dependant control of fatty acid derivative producing proteins. Second, AND-gate dynamic controllers that combine inducible promoter function, which enables cells to quickly accumulate biomass before triggering the production of biofuel producing proteins, and fatty acid sensing-regulation were constructed. Third, xylose sensor-regulators were created, where xylose is a major sugar component in the affordable lignocellulose biomass carbon source. This allows regulation of xylose utilizing proteins based on the amount of xylose sugars detected. The function and performance of these synthetic promoters to dynamically control yEGFP reporter protein were demonstrated. DOCTOR OF PHILOSOPHY (SCBE) 2014-09-12T04:04:15Z 2014-09-12T04:04:15Z 2014 2014 Thesis Teo, W. S. (2014). Engineering synthetic promoters as dynamic controllers in saccharomyces cerevisiae. Doctoral thesis, Nanyang Technological University, Singapore. https://hdl.handle.net/10356/61754 10.32657/10356/61754 en 157 p. application/pdf |
| spellingShingle | DRNTU::Engineering::Chemical engineering::Biotechnology DRNTU::Engineering::Chemical engineering::Biochemical engineering Teo, Wei Suong Engineering synthetic promoters as dynamic controllers in saccharomyces cerevisiae |
| title | Engineering synthetic promoters as dynamic controllers in saccharomyces cerevisiae |
| title_full | Engineering synthetic promoters as dynamic controllers in saccharomyces cerevisiae |
| title_fullStr | Engineering synthetic promoters as dynamic controllers in saccharomyces cerevisiae |
| title_full_unstemmed | Engineering synthetic promoters as dynamic controllers in saccharomyces cerevisiae |
| title_short | Engineering synthetic promoters as dynamic controllers in saccharomyces cerevisiae |
| title_sort | engineering synthetic promoters as dynamic controllers in saccharomyces cerevisiae |
| topic | DRNTU::Engineering::Chemical engineering::Biotechnology DRNTU::Engineering::Chemical engineering::Biochemical engineering |
| url | https://hdl.handle.net/10356/61754 |
| work_keys_str_mv | AT teoweisuong engineeringsyntheticpromotersasdynamiccontrollersinsaccharomycescerevisiae |