Metabolic regulator as ECM role for liver cells

ECM substrates have been recognized as the key biological materials that promote in vitro liver tissue formation. In nature tissue, ECM substrates present both in between cells and cells to epitheliums. They are not only providing biophysical support, but also biochemical cues that regulate cell maj...

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Main Author: Loh, Chun Keat
Other Authors: Cho Nam Joon
Format: Final Year Project (FYP)
Language:English
Published: 2015
Subjects:
Online Access:http://hdl.handle.net/10356/62425
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author Loh, Chun Keat
author2 Cho Nam Joon
author_facet Cho Nam Joon
Loh, Chun Keat
author_sort Loh, Chun Keat
collection NTU
description ECM substrates have been recognized as the key biological materials that promote in vitro liver tissue formation. In nature tissue, ECM substrates present both in between cells and cells to epitheliums. They are not only providing biophysical support, but also biochemical cues that regulate cell major activities such as proliferation, migration and differentiation. Integrins bind with specific ECM substrates initiate intracellular signals transduction to the nucleus for response. Several mediators and growth factors such as cytokines and hormones are in phase in facilitating this process. Our project aims to study the individual impact of three main ECM substrates, collagen, fibronectin and laminin to liver cell proliferation and metabolism. The literature review provides insights on the mechanism of liver regeneration, metabolism and signal transduction. The role of ECM matrix as a whole is also discussed. Huh 7.5, one type of hepatocellular carcinoma is used as the cell line in this project. Cell proliferation number directly indicates the effect of ECM substrates to cell growth. Protein and gene expression of hepatocytes specific biomarkers indicate the functions which the cells are undergoing. Cell proliferation cycle, antigen-antibody specificity of proteins, and reverse transcription mechanism of DNA are the important concepts that support our experiments. The results indicate that all three ECM substrates encourage cell growth. Albumin consistently shows positive expression in both qualitative and quantitative analysis under collagen and laminin substrates. Also, Huh 7.5 cells shows variation in cell size and shape under different ECM substrates. Details of results can be found in later portion of the report.
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spelling ntu-10356/624252023-03-04T15:43:10Z Metabolic regulator as ECM role for liver cells Loh, Chun Keat Cho Nam Joon School of Materials Science and Engineering Translational Science Group DRNTU::Engineering::Materials::Biomaterials ECM substrates have been recognized as the key biological materials that promote in vitro liver tissue formation. In nature tissue, ECM substrates present both in between cells and cells to epitheliums. They are not only providing biophysical support, but also biochemical cues that regulate cell major activities such as proliferation, migration and differentiation. Integrins bind with specific ECM substrates initiate intracellular signals transduction to the nucleus for response. Several mediators and growth factors such as cytokines and hormones are in phase in facilitating this process. Our project aims to study the individual impact of three main ECM substrates, collagen, fibronectin and laminin to liver cell proliferation and metabolism. The literature review provides insights on the mechanism of liver regeneration, metabolism and signal transduction. The role of ECM matrix as a whole is also discussed. Huh 7.5, one type of hepatocellular carcinoma is used as the cell line in this project. Cell proliferation number directly indicates the effect of ECM substrates to cell growth. Protein and gene expression of hepatocytes specific biomarkers indicate the functions which the cells are undergoing. Cell proliferation cycle, antigen-antibody specificity of proteins, and reverse transcription mechanism of DNA are the important concepts that support our experiments. The results indicate that all three ECM substrates encourage cell growth. Albumin consistently shows positive expression in both qualitative and quantitative analysis under collagen and laminin substrates. Also, Huh 7.5 cells shows variation in cell size and shape under different ECM substrates. Details of results can be found in later portion of the report. Bachelor of Engineering (Materials Engineering) 2015-04-04T02:35:43Z 2015-04-04T02:35:43Z 2015 2015 Final Year Project (FYP) http://hdl.handle.net/10356/62425 en Nanyang Technological University 52 p. application/pdf
spellingShingle DRNTU::Engineering::Materials::Biomaterials
Loh, Chun Keat
Metabolic regulator as ECM role for liver cells
title Metabolic regulator as ECM role for liver cells
title_full Metabolic regulator as ECM role for liver cells
title_fullStr Metabolic regulator as ECM role for liver cells
title_full_unstemmed Metabolic regulator as ECM role for liver cells
title_short Metabolic regulator as ECM role for liver cells
title_sort metabolic regulator as ecm role for liver cells
topic DRNTU::Engineering::Materials::Biomaterials
url http://hdl.handle.net/10356/62425
work_keys_str_mv AT lohchunkeat metabolicregulatorasecmroleforlivercells