Potential downstream targets of the polyamine pathway and their roles in embryonic stem cell renewal
Embryonic stem cells hold great potential for regenerative medicine due to their ability to self-renew and differentiate into cells of all embryonic cell lineages. Polyamines are cationic molecules consisting of putrescine, spermidine and spermine A polyamine synthesis regulator, adenosylmethionine...
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| Format: | Final Year Project (FYP) |
| Language: | English |
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2015
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| Online Access: | http://hdl.handle.net/10356/63170 |
| _version_ | 1811690784182763520 |
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| author | Fong, Koon Kit |
| author2 | Leah Vardy |
| author_facet | Leah Vardy Fong, Koon Kit |
| author_sort | Fong, Koon Kit |
| collection | NTU |
| description | Embryonic stem cells hold great potential for regenerative medicine due to their ability to self-renew and differentiate into cells of all embryonic cell lineages. Polyamines are cationic molecules consisting of putrescine, spermidine and spermine A polyamine synthesis regulator, adenosylmethionine decarboxylase 1 (AMD1) was shown to be essential for embryonic stem cell self-renewal. A microarray was performed to identify genes that were downregulated on ESC differentiation while upregulated upon AMD1 overexpression. These genes are potential downstream targets of the polyamine pathway that could regulate embryonic stem cell self-renewal. Functional assays of overexpression and knockdown were performed for the candidate genes of Aqp3 and Zmat4. Aqp3 and Zmat4 isoforms were overexpressed and knocked down using siRNA. Core pluripotency genes and differentiation markers were examined by qRT-PCR while pluripotency was tested with alkaline phosphatase staining. Overexpression and knockdown results suggest that Aqp3 and Zmat4 did not have a significant impact on pluripotency. However, more experiments are required to have a definitive conclusion. |
| first_indexed | 2024-10-01T06:09:30Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/63170 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:09:30Z |
| publishDate | 2015 |
| record_format | dspace |
| spelling | ntu-10356/631702023-02-28T18:05:20Z Potential downstream targets of the polyamine pathway and their roles in embryonic stem cell renewal Fong, Koon Kit Leah Vardy School of Biological Sciences A*STAR Institute of Medical Biology DRNTU::Science::Biological sciences::Molecular biology Embryonic stem cells hold great potential for regenerative medicine due to their ability to self-renew and differentiate into cells of all embryonic cell lineages. Polyamines are cationic molecules consisting of putrescine, spermidine and spermine A polyamine synthesis regulator, adenosylmethionine decarboxylase 1 (AMD1) was shown to be essential for embryonic stem cell self-renewal. A microarray was performed to identify genes that were downregulated on ESC differentiation while upregulated upon AMD1 overexpression. These genes are potential downstream targets of the polyamine pathway that could regulate embryonic stem cell self-renewal. Functional assays of overexpression and knockdown were performed for the candidate genes of Aqp3 and Zmat4. Aqp3 and Zmat4 isoforms were overexpressed and knocked down using siRNA. Core pluripotency genes and differentiation markers were examined by qRT-PCR while pluripotency was tested with alkaline phosphatase staining. Overexpression and knockdown results suggest that Aqp3 and Zmat4 did not have a significant impact on pluripotency. However, more experiments are required to have a definitive conclusion. Bachelor of Science in Biological Sciences 2015-05-08T04:00:20Z 2015-05-08T04:00:20Z 2015 2015 Final Year Project (FYP) http://hdl.handle.net/10356/63170 en Nanyang Technological University 41 p. application/pdf |
| spellingShingle | DRNTU::Science::Biological sciences::Molecular biology Fong, Koon Kit Potential downstream targets of the polyamine pathway and their roles in embryonic stem cell renewal |
| title | Potential downstream targets of the polyamine pathway and their roles in embryonic stem cell renewal |
| title_full | Potential downstream targets of the polyamine pathway and their roles in embryonic stem cell renewal |
| title_fullStr | Potential downstream targets of the polyamine pathway and their roles in embryonic stem cell renewal |
| title_full_unstemmed | Potential downstream targets of the polyamine pathway and their roles in embryonic stem cell renewal |
| title_short | Potential downstream targets of the polyamine pathway and their roles in embryonic stem cell renewal |
| title_sort | potential downstream targets of the polyamine pathway and their roles in embryonic stem cell renewal |
| topic | DRNTU::Science::Biological sciences::Molecular biology |
| url | http://hdl.handle.net/10356/63170 |
| work_keys_str_mv | AT fongkoonkit potentialdownstreamtargetsofthepolyaminepathwayandtheirrolesinembryonicstemcellrenewal |