Molecular characterization of cellular stress responses during coronavirus infection
Coronaviruses are important animal and human pathogens. In this study, two stress pathways – the unfolded protein response (UPR) and c-Jun N-terminal kinase (JNK) pathway are shown to be activated in cells infected with infectious bronchitis virus (IBV). The inositol requiring protein 1 (IRE1) branc...
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| Format: | Thesis |
| Language: | English |
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2015
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| Online Access: | http://hdl.handle.net/10356/63947 |
| _version_ | 1826109743272296448 |
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| author | Fung, To Sing |
| author2 | Liu Ding Xiang |
| author_facet | Liu Ding Xiang Fung, To Sing |
| author_sort | Fung, To Sing |
| collection | NTU |
| description | Coronaviruses are important animal and human pathogens. In this study, two stress pathways – the unfolded protein response (UPR) and c-Jun N-terminal kinase (JNK) pathway are shown to be activated in cells infected with infectious bronchitis virus (IBV). The inositol requiring protein 1 (IRE1) branch of UPR protects infected cells from apoptosis by splicing the mRNA of X-box protein 1 (XBP1) and differentially modulating the activation of two kinases. The PKR-like ER protein kinase (PERK) branch of UPR promotes IBV-induced apoptosis by up-regulating C/EBP homologous protein (CHOP). JNK is phosphorylated by MKK7 during IBV infection, and it promotes apoptosis by modulating the level of B cell lymphoma-2 (Bcl2) family proteins. Moreover, IRE1 mediates autophagy induction whereas XBP1 and JNK contribute to the induction of pro-inflammatory cytokines (interleukin-8) and type-I interferon in the IBV-infected cells. Therefore, these stress pathways modulate critical cellular events and contribute to pathogenesis during coronavirus infection. |
| first_indexed | 2024-10-01T02:23:19Z |
| format | Thesis |
| id | ntu-10356/63947 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T02:23:19Z |
| publishDate | 2015 |
| record_format | dspace |
| spelling | ntu-10356/639472023-02-28T18:31:02Z Molecular characterization of cellular stress responses during coronavirus infection Fung, To Sing Liu Ding Xiang School of Biological Sciences DRNTU::Science::Biological sciences::Microbiology::Virology Coronaviruses are important animal and human pathogens. In this study, two stress pathways – the unfolded protein response (UPR) and c-Jun N-terminal kinase (JNK) pathway are shown to be activated in cells infected with infectious bronchitis virus (IBV). The inositol requiring protein 1 (IRE1) branch of UPR protects infected cells from apoptosis by splicing the mRNA of X-box protein 1 (XBP1) and differentially modulating the activation of two kinases. The PKR-like ER protein kinase (PERK) branch of UPR promotes IBV-induced apoptosis by up-regulating C/EBP homologous protein (CHOP). JNK is phosphorylated by MKK7 during IBV infection, and it promotes apoptosis by modulating the level of B cell lymphoma-2 (Bcl2) family proteins. Moreover, IRE1 mediates autophagy induction whereas XBP1 and JNK contribute to the induction of pro-inflammatory cytokines (interleukin-8) and type-I interferon in the IBV-infected cells. Therefore, these stress pathways modulate critical cellular events and contribute to pathogenesis during coronavirus infection. Doctor of Philosophy (SBS) 2015-05-20T08:12:34Z 2015-05-20T08:12:34Z 2015 2015 Thesis Fung, T. S. (2015). Molecular characterization of cellular stress responses during coronavirus infection. Doctoral thesis, Nanyang Technological University, Singapore. http://hdl.handle.net/10356/63947 en 232 p. application/pdf |
| spellingShingle | DRNTU::Science::Biological sciences::Microbiology::Virology Fung, To Sing Molecular characterization of cellular stress responses during coronavirus infection |
| title | Molecular characterization of cellular stress responses during coronavirus infection |
| title_full | Molecular characterization of cellular stress responses during coronavirus infection |
| title_fullStr | Molecular characterization of cellular stress responses during coronavirus infection |
| title_full_unstemmed | Molecular characterization of cellular stress responses during coronavirus infection |
| title_short | Molecular characterization of cellular stress responses during coronavirus infection |
| title_sort | molecular characterization of cellular stress responses during coronavirus infection |
| topic | DRNTU::Science::Biological sciences::Microbiology::Virology |
| url | http://hdl.handle.net/10356/63947 |
| work_keys_str_mv | AT fungtosing molecularcharacterizationofcellularstressresponsesduringcoronavirusinfection |