Droplet-base microfluidics single cell isolation
Interpretation of DNA profiles is a critical step in the course of criminal investigation. The technical skills involved requires analyst to draw the skills acquired through rigorous training and experience. Arguably, the most challenging DNA profile interpretation are complex mixtures with unknown...
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| Other Authors: | |
| Format: | Thesis |
| Language: | English |
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2015
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| Online Access: | http://hdl.handle.net/10356/64399 |
| _version_ | 1826114415563374592 |
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| author | Choi, Kwok Keong |
| author2 | Kang Yuejun |
| author_facet | Kang Yuejun Choi, Kwok Keong |
| author_sort | Choi, Kwok Keong |
| collection | NTU |
| description | Interpretation of DNA profiles is a critical step in the course of criminal investigation. The technical skills involved requires analyst to draw the skills acquired through rigorous training and experience. Arguably, the most challenging DNA profile interpretation are complex mixtures with unknown number of contributors or without reference samples for elimination. Although guidelines were established in the forensics community, the protocols clearly lack robustness and could lead to potential wide range of interpretations. In this study, we seek to resolve samples consisting of cells contributed from more than a single source by individualizing of cells present through the application of droplet microfluidics. Two selected microchip designs were fabricated with soft lithography for isolation of single cells through encapsulation in oil/water emulsion. To achieve this objective, we study and optimized the flow rates and oil:water ratios for operation in the respective microchips. The intend of this study is to spearhead the concept of single cell isolation prior to standard downstream DNA profiling protocols for the purpose of complex mixture resolution. |
| first_indexed | 2024-10-01T03:38:47Z |
| format | Thesis |
| id | ntu-10356/64399 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:38:47Z |
| publishDate | 2015 |
| record_format | dspace |
| spelling | ntu-10356/643992023-03-03T15:59:21Z Droplet-base microfluidics single cell isolation Choi, Kwok Keong Kang Yuejun School of Chemical and Biomedical Engineering DRNTU::Engineering::Bioengineering Interpretation of DNA profiles is a critical step in the course of criminal investigation. The technical skills involved requires analyst to draw the skills acquired through rigorous training and experience. Arguably, the most challenging DNA profile interpretation are complex mixtures with unknown number of contributors or without reference samples for elimination. Although guidelines were established in the forensics community, the protocols clearly lack robustness and could lead to potential wide range of interpretations. In this study, we seek to resolve samples consisting of cells contributed from more than a single source by individualizing of cells present through the application of droplet microfluidics. Two selected microchip designs were fabricated with soft lithography for isolation of single cells through encapsulation in oil/water emulsion. To achieve this objective, we study and optimized the flow rates and oil:water ratios for operation in the respective microchips. The intend of this study is to spearhead the concept of single cell isolation prior to standard downstream DNA profiling protocols for the purpose of complex mixture resolution. Master of Science (Biomedical Engineering) 2015-05-26T06:45:22Z 2015-05-26T06:45:22Z 2015 2015 Thesis http://hdl.handle.net/10356/64399 en 45 p. application/pdf |
| spellingShingle | DRNTU::Engineering::Bioengineering Choi, Kwok Keong Droplet-base microfluidics single cell isolation |
| title | Droplet-base microfluidics single cell isolation |
| title_full | Droplet-base microfluidics single cell isolation |
| title_fullStr | Droplet-base microfluidics single cell isolation |
| title_full_unstemmed | Droplet-base microfluidics single cell isolation |
| title_short | Droplet-base microfluidics single cell isolation |
| title_sort | droplet base microfluidics single cell isolation |
| topic | DRNTU::Engineering::Bioengineering |
| url | http://hdl.handle.net/10356/64399 |
| work_keys_str_mv | AT choikwokkeong dropletbasemicrofluidicssinglecellisolation |