Structural biology of proteins involved in the replication of herpesviruses
Production of new infectious herpesvirus particles is dependent on the disruptions of host immune responses, herpesvirus DNA replication, assembly and reactivation from latency. To better understand herpesvirus replication, crystallographic and interaction studies were performed on several key prote...
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| Other Authors: | |
| Format: | Thesis |
| Language: | English |
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2015
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| Online Access: | http://hdl.handle.net/10356/65239 |
| _version_ | 1811679590762938368 |
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| author | Hew, Kelly Kai Li |
| author2 | Nordlund, Pär |
| author_facet | Nordlund, Pär Hew, Kelly Kai Li |
| author_sort | Hew, Kelly Kai Li |
| collection | NTU |
| description | Production of new infectious herpesvirus particles is dependent on the disruptions of host immune responses, herpesvirus DNA replication, assembly and reactivation from latency. To better understand herpesvirus replication, crystallographic and interaction studies were performed on several key proteins. This includes Kaposi’s sarcoma associated herpesvirus (KSHV) viral interferon regulatory factor 1 (vIRF-1), varicella zoster virus encoded thymidylate synthase (TS), tegument protein VP22 from herpes simplex virus 1 and KSHV-encoded ORF49. The structural and biophysical analyzes of the proteins provided structural and mechanistic insights and revealed multiple novel interactions with previously unknown partners. The VP22 structure established a previously unknown structural homology with another tegument protein ORF52. The complex structures of TS and vIRF-1 illustrated similar molecular details in substrate binding as their human counterparts. The work therefore contributes to a more comprehensive understanding of herpesvirus replication, reinforcing the importance of structural studies to understand molecular aspects of herpesvirus function. |
| first_indexed | 2024-10-01T03:11:35Z |
| format | Thesis |
| id | ntu-10356/65239 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:11:35Z |
| publishDate | 2015 |
| record_format | dspace |
| spelling | ntu-10356/652392023-02-28T18:33:45Z Structural biology of proteins involved in the replication of herpesviruses Hew, Kelly Kai Li Nordlund, Pär School of Biological Sciences DRNTU::Science::Biological sciences::Microbiology::Virology DRNTU::Science::Biological sciences::Biophysics DRNTU::Science::Biological sciences::Biochemistry Production of new infectious herpesvirus particles is dependent on the disruptions of host immune responses, herpesvirus DNA replication, assembly and reactivation from latency. To better understand herpesvirus replication, crystallographic and interaction studies were performed on several key proteins. This includes Kaposi’s sarcoma associated herpesvirus (KSHV) viral interferon regulatory factor 1 (vIRF-1), varicella zoster virus encoded thymidylate synthase (TS), tegument protein VP22 from herpes simplex virus 1 and KSHV-encoded ORF49. The structural and biophysical analyzes of the proteins provided structural and mechanistic insights and revealed multiple novel interactions with previously unknown partners. The VP22 structure established a previously unknown structural homology with another tegument protein ORF52. The complex structures of TS and vIRF-1 illustrated similar molecular details in substrate binding as their human counterparts. The work therefore contributes to a more comprehensive understanding of herpesvirus replication, reinforcing the importance of structural studies to understand molecular aspects of herpesvirus function. Doctor of Philosophy (SBS) 2015-06-17T03:37:47Z 2015-06-17T03:37:47Z 2015 2015 Thesis Hew, K. K. L. (2015). Structural biology of proteins involved in the replication of herpesviruses. Doctoral thesis, Nanyang Technological University, Singapore. http://hdl.handle.net/10356/65239 en 133 p. application/pdf |
| spellingShingle | DRNTU::Science::Biological sciences::Microbiology::Virology DRNTU::Science::Biological sciences::Biophysics DRNTU::Science::Biological sciences::Biochemistry Hew, Kelly Kai Li Structural biology of proteins involved in the replication of herpesviruses |
| title | Structural biology of proteins involved in the replication of herpesviruses |
| title_full | Structural biology of proteins involved in the replication of herpesviruses |
| title_fullStr | Structural biology of proteins involved in the replication of herpesviruses |
| title_full_unstemmed | Structural biology of proteins involved in the replication of herpesviruses |
| title_short | Structural biology of proteins involved in the replication of herpesviruses |
| title_sort | structural biology of proteins involved in the replication of herpesviruses |
| topic | DRNTU::Science::Biological sciences::Microbiology::Virology DRNTU::Science::Biological sciences::Biophysics DRNTU::Science::Biological sciences::Biochemistry |
| url | http://hdl.handle.net/10356/65239 |
| work_keys_str_mv | AT hewkellykaili structuralbiologyofproteinsinvolvedinthereplicationofherpesviruses |