LIMK1 regulates centrosome integrity and mitotic spindle positioning
Actin cytoskeleton dynamics and proteins associated with actin cytoskeleton have been reported to play a role in regulating the cell cycle. LIM motif containing protein kinase 1 (LIMK1), a kinase involved in regulating actin cytoskeleton, has been reported to regulate cell cycle progression and asso...
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| Format: | Thesis |
| Language: | English |
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2015
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| Online Access: | https://hdl.handle.net/10356/65350 |
| _version_ | 1826124518314213376 |
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| author | Ou, Sirong |
| author2 | Koh Cheng Gee |
| author_facet | Koh Cheng Gee Ou, Sirong |
| author_sort | Ou, Sirong |
| collection | NTU |
| description | Actin cytoskeleton dynamics and proteins associated with actin cytoskeleton have been reported to play a role in regulating the cell cycle. LIM motif containing protein kinase 1 (LIMK1), a kinase involved in regulating actin cytoskeleton, has been reported to regulate cell cycle progression and associate with the centrosome during M-phase. However, the role of LIMK1 at the centrosome is not fully understood. Here, we reported that LIMK1 is involved in regulating centrosome integrity during M-phase. Cytoplasmic dynein light intermediate chain 1 and 2 (DYNC1LI1 and DYNC1LI2) are potential substrates functioning downstream of LIMK1 in centrosome integrity maintenance. LIMK1 mediated phosphorylation of DYNC1LIs could potentially regulate the transportation of centrosomal proteins to the spindle poles. Besides regulating centrosome integrity, LIMK1-Cofilin could potentially regulate the cortical actin network for proper spindle orientation and positioning. |
| first_indexed | 2024-10-01T06:21:37Z |
| format | Thesis |
| id | ntu-10356/65350 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:21:37Z |
| publishDate | 2015 |
| record_format | dspace |
| spelling | ntu-10356/653502023-02-28T18:43:44Z LIMK1 regulates centrosome integrity and mitotic spindle positioning Ou, Sirong Koh Cheng Gee School of Biological Sciences DRNTU::Science::Biological sciences::Molecular biology Actin cytoskeleton dynamics and proteins associated with actin cytoskeleton have been reported to play a role in regulating the cell cycle. LIM motif containing protein kinase 1 (LIMK1), a kinase involved in regulating actin cytoskeleton, has been reported to regulate cell cycle progression and associate with the centrosome during M-phase. However, the role of LIMK1 at the centrosome is not fully understood. Here, we reported that LIMK1 is involved in regulating centrosome integrity during M-phase. Cytoplasmic dynein light intermediate chain 1 and 2 (DYNC1LI1 and DYNC1LI2) are potential substrates functioning downstream of LIMK1 in centrosome integrity maintenance. LIMK1 mediated phosphorylation of DYNC1LIs could potentially regulate the transportation of centrosomal proteins to the spindle poles. Besides regulating centrosome integrity, LIMK1-Cofilin could potentially regulate the cortical actin network for proper spindle orientation and positioning. Doctor of Philosophy 2015-08-06T03:43:51Z 2015-08-06T03:43:51Z 2015 2015 Thesis Ou, S. (2015). LIMK1 regulates centrosome integrity and mitotic spindle positioning. Doctoral thesis, Nanyang Technological University, Singapore. https://hdl.handle.net/10356/65350 10.32657/10356/65350 en 237 p. application/pdf |
| spellingShingle | DRNTU::Science::Biological sciences::Molecular biology Ou, Sirong LIMK1 regulates centrosome integrity and mitotic spindle positioning |
| title | LIMK1 regulates centrosome integrity and mitotic spindle positioning |
| title_full | LIMK1 regulates centrosome integrity and mitotic spindle positioning |
| title_fullStr | LIMK1 regulates centrosome integrity and mitotic spindle positioning |
| title_full_unstemmed | LIMK1 regulates centrosome integrity and mitotic spindle positioning |
| title_short | LIMK1 regulates centrosome integrity and mitotic spindle positioning |
| title_sort | limk1 regulates centrosome integrity and mitotic spindle positioning |
| topic | DRNTU::Science::Biological sciences::Molecular biology |
| url | https://hdl.handle.net/10356/65350 |
| work_keys_str_mv | AT ousirong limk1regulatescentrosomeintegrityandmitoticspindlepositioning |