Studies of the molecules form plasmodium falciparum that mediate pathogenesis
Adhesion of erythrocytes infected by Plasmodium falciparum to receptors of the microvasculature is a major contributor of parasite pathology and morbidity. It is mediated by the P. falciparum erythrocyte membrane protein 1 (PfEMP-1) which is expressed at the surface of infected erythocytes and is li...
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| Format: | Thesis |
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2008
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| Online Access: | https://hdl.handle.net/10356/6566 |
| _version_ | 1826120187926020096 |
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| author | Mo, Min |
| author2 | Julien Lescar |
| author_facet | Julien Lescar Mo, Min |
| author_sort | Mo, Min |
| collection | NTU |
| description | Adhesion of erythrocytes infected by Plasmodium falciparum to receptors of the microvasculature is a major contributor of parasite pathology and morbidity. It is mediated by the P. falciparum erythrocyte membrane protein 1 (PfEMP-1) which is expressed at the surface of infected erythocytes and is linked to both antigenic variation and cytoadherence. The PfEMP-1 protein contains multiple adhesive modules, including the cysteine-rich interdomain region (CIDR). The interaction between CIDRa and CD36 promotes stable adherence of parasitized erythrocytes to endothelial cells. Here we show that a segment within the C-terminal region of CIDRa determines CD36 binding specificity. Antibodies raised against this segment can specifically block the adhesion of various parasite stains to CD36. Thus, small regions of PfEMP-1 that determine binding specificity could form suitable components of an anti-sequestration malaria vaccine effective against different parasite strains. |
| first_indexed | 2024-10-01T05:12:26Z |
| format | Thesis |
| id | ntu-10356/6566 |
| institution | Nanyang Technological University |
| last_indexed | 2024-10-01T05:12:26Z |
| publishDate | 2008 |
| record_format | dspace |
| spelling | ntu-10356/65662023-02-28T18:39:52Z Studies of the molecules form plasmodium falciparum that mediate pathogenesis Mo, Min Julien Lescar School of Biological Sciences DRNTU::Science::Biological sciences Adhesion of erythrocytes infected by Plasmodium falciparum to receptors of the microvasculature is a major contributor of parasite pathology and morbidity. It is mediated by the P. falciparum erythrocyte membrane protein 1 (PfEMP-1) which is expressed at the surface of infected erythocytes and is linked to both antigenic variation and cytoadherence. The PfEMP-1 protein contains multiple adhesive modules, including the cysteine-rich interdomain region (CIDR). The interaction between CIDRa and CD36 promotes stable adherence of parasitized erythrocytes to endothelial cells. Here we show that a segment within the C-terminal region of CIDRa determines CD36 binding specificity. Antibodies raised against this segment can specifically block the adhesion of various parasite stains to CD36. Thus, small regions of PfEMP-1 that determine binding specificity could form suitable components of an anti-sequestration malaria vaccine effective against different parasite strains. DOCTOR OF PHILOSOPHY (SBS) 2008-09-17T11:41:55Z 2008-09-17T11:41:55Z 2007 2007 Thesis Mo, M. (2007). Studies of the molecules form plasmodium falciparum that mediate pathogenesis. Doctoral thesis, Nanyang Technological University, Singapore. https://hdl.handle.net/10356/6566 10.32657/10356/6566 Nanyang Technological University application/pdf |
| spellingShingle | DRNTU::Science::Biological sciences Mo, Min Studies of the molecules form plasmodium falciparum that mediate pathogenesis |
| title | Studies of the molecules form plasmodium falciparum that mediate pathogenesis |
| title_full | Studies of the molecules form plasmodium falciparum that mediate pathogenesis |
| title_fullStr | Studies of the molecules form plasmodium falciparum that mediate pathogenesis |
| title_full_unstemmed | Studies of the molecules form plasmodium falciparum that mediate pathogenesis |
| title_short | Studies of the molecules form plasmodium falciparum that mediate pathogenesis |
| title_sort | studies of the molecules form plasmodium falciparum that mediate pathogenesis |
| topic | DRNTU::Science::Biological sciences |
| url | https://hdl.handle.net/10356/6566 |
| work_keys_str_mv | AT momin studiesofthemoleculesformplasmodiumfalciparumthatmediatepathogenesis |