Incorporation of modified bases into triplex-forming peptide nucleic acids for the recognition of a C-G pyrimidine-purine inversion site of an RNA duplex
Peptide nucleic acid (PNA), an analogue of DNA, can enhance the recognition of specific sequences in RNA duplexes and may be useful therapeutic reagents. In this study, a PNA monomer (Q) containing a N4-(2-guanidoethyl)-5-methylcytosine base was synthesized and incorporated into 8-mer PNAs. Togethe...
| Main Author: | |
|---|---|
| Other Authors: | |
| Format: | Thesis |
| Language: | English |
| Published: |
2015
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/10356/65663 |
| _version_ | 1811691912056274944 |
|---|---|
| author | Toh, Desiree-Faye Kaixin |
| author2 | Chen Gang |
| author_facet | Chen Gang Toh, Desiree-Faye Kaixin |
| author_sort | Toh, Desiree-Faye Kaixin |
| collection | NTU |
| description | Peptide nucleic acid (PNA), an analogue of DNA, can enhance the recognition of specific sequences in RNA duplexes and may be useful therapeutic reagents. In this study, a PNA monomer (Q) containing a N4-(2-guanidoethyl)-5-methylcytosine base was synthesized and incorporated into 8-mer PNAs. Together with previously reported thio-pseudoisocytosine (L) monomer, the non-denaturing polyacrylamide gel electrophoresis results suggest that the PNA is able to selectively target RNA hairpins containing a pyrimidine-purine (C-G) inversion with high binding affinity. The PNA does not bind to DNA hairpins. Thermal melting experiments indicate that PNA containing Q monomer is not able to bind to single-stranded RNA. |
| first_indexed | 2024-10-01T06:27:25Z |
| format | Thesis |
| id | ntu-10356/65663 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:27:25Z |
| publishDate | 2015 |
| record_format | dspace |
| spelling | ntu-10356/656632023-02-28T23:50:16Z Incorporation of modified bases into triplex-forming peptide nucleic acids for the recognition of a C-G pyrimidine-purine inversion site of an RNA duplex Toh, Desiree-Faye Kaixin Chen Gang School of Physical and Mathematical Sciences DRNTU::Science::Chemistry::Biochemistry Peptide nucleic acid (PNA), an analogue of DNA, can enhance the recognition of specific sequences in RNA duplexes and may be useful therapeutic reagents. In this study, a PNA monomer (Q) containing a N4-(2-guanidoethyl)-5-methylcytosine base was synthesized and incorporated into 8-mer PNAs. Together with previously reported thio-pseudoisocytosine (L) monomer, the non-denaturing polyacrylamide gel electrophoresis results suggest that the PNA is able to selectively target RNA hairpins containing a pyrimidine-purine (C-G) inversion with high binding affinity. The PNA does not bind to DNA hairpins. Thermal melting experiments indicate that PNA containing Q monomer is not able to bind to single-stranded RNA. Master of Science 2015-12-03T08:39:06Z 2015-12-03T08:39:06Z 2015 2015 Thesis Toh, D.-F. K. (2015). Incorporation of modified bases into triplex-forming peptide nucleic acids for the recognition of a C-G pyrimidine-purine inversion site of an RNA duplex. Master's thesis, Nanyang Technological University, Singapore. http://hdl.handle.net/10356/65663 en 52 p. application/pdf |
| spellingShingle | DRNTU::Science::Chemistry::Biochemistry Toh, Desiree-Faye Kaixin Incorporation of modified bases into triplex-forming peptide nucleic acids for the recognition of a C-G pyrimidine-purine inversion site of an RNA duplex |
| title | Incorporation of modified bases into triplex-forming peptide nucleic acids for the recognition of a C-G pyrimidine-purine inversion site of an RNA duplex |
| title_full | Incorporation of modified bases into triplex-forming peptide nucleic acids for the recognition of a C-G pyrimidine-purine inversion site of an RNA duplex |
| title_fullStr | Incorporation of modified bases into triplex-forming peptide nucleic acids for the recognition of a C-G pyrimidine-purine inversion site of an RNA duplex |
| title_full_unstemmed | Incorporation of modified bases into triplex-forming peptide nucleic acids for the recognition of a C-G pyrimidine-purine inversion site of an RNA duplex |
| title_short | Incorporation of modified bases into triplex-forming peptide nucleic acids for the recognition of a C-G pyrimidine-purine inversion site of an RNA duplex |
| title_sort | incorporation of modified bases into triplex forming peptide nucleic acids for the recognition of a c g pyrimidine purine inversion site of an rna duplex |
| topic | DRNTU::Science::Chemistry::Biochemistry |
| url | http://hdl.handle.net/10356/65663 |
| work_keys_str_mv | AT tohdesireefayekaixin incorporationofmodifiedbasesintotriplexformingpeptidenucleicacidsfortherecognitionofacgpyrimidinepurineinversionsiteofanrnaduplex |