Titanium dioxide and zinc oxide nanoparticles induce autophagy associated with toxicity effects in primary human keratinocytes : a dose relevance study
The application of nanomaterials in cosmetic, sunscreens, and topical products is growing rapidly, even as health concerns arise about the potential hazards to their use. This research work is centered on the toxicological effects of titanium dioxide (TiO2) and zinc oxide (ZnO) nanoparticles, which...
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| Format: | Thesis |
| Language: | English |
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2015
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| Online Access: | https://hdl.handle.net/10356/65668 |
| _version_ | 1811677228409290752 |
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| author | Zhao, Yun |
| author2 | Ng Kee Woei |
| author_facet | Ng Kee Woei Zhao, Yun |
| author_sort | Zhao, Yun |
| collection | NTU |
| description | The application of nanomaterials in cosmetic, sunscreens, and topical products is growing rapidly, even as health concerns arise about the potential hazards to their use. This research work is centered on the toxicological effects of titanium dioxide (TiO2) and zinc oxide (ZnO) nanoparticles, which are commonly used in sunscreens, in the interaction with primary human keratinocytes. Both TiO2 and ZnO nanoparticles are found to cause toxicity in vitro, such as cytotoxicity, oxidative stress, genotoxicity, and inflammasome activation in a concentration-dependent manner through conducting a dose relevance study. Furthermore, TiO2 and ZnO nanoparticles are capable of inducing autophagy within a low subtoxic dose range. This is the first study of autophagy induced in primary human keratinocytes by nano TiO2 and ZnO without other stimuli. The low triggering dose range of autophagy found in keratinocytes would be useful impetus for the research community to look more into the safety limits of nanomaterials applied to skin products. |
| first_indexed | 2024-10-01T02:34:02Z |
| format | Thesis |
| id | ntu-10356/65668 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T02:34:02Z |
| publishDate | 2015 |
| record_format | dspace |
| spelling | ntu-10356/656682023-03-04T16:42:10Z Titanium dioxide and zinc oxide nanoparticles induce autophagy associated with toxicity effects in primary human keratinocytes : a dose relevance study Zhao, Yun Ng Kee Woei School of Materials Science & Engineering DRNTU::Engineering::Nanotechnology The application of nanomaterials in cosmetic, sunscreens, and topical products is growing rapidly, even as health concerns arise about the potential hazards to their use. This research work is centered on the toxicological effects of titanium dioxide (TiO2) and zinc oxide (ZnO) nanoparticles, which are commonly used in sunscreens, in the interaction with primary human keratinocytes. Both TiO2 and ZnO nanoparticles are found to cause toxicity in vitro, such as cytotoxicity, oxidative stress, genotoxicity, and inflammasome activation in a concentration-dependent manner through conducting a dose relevance study. Furthermore, TiO2 and ZnO nanoparticles are capable of inducing autophagy within a low subtoxic dose range. This is the first study of autophagy induced in primary human keratinocytes by nano TiO2 and ZnO without other stimuli. The low triggering dose range of autophagy found in keratinocytes would be useful impetus for the research community to look more into the safety limits of nanomaterials applied to skin products. DOCTOR OF PHILOSOPHY (MSE) 2015-12-05T03:07:21Z 2015-12-05T03:07:21Z 2015 2015 Thesis Zhao, Y. (2015). Titanium dioxide and zinc oxide nanoparticles induce autophagy associated with toxicity effects in primary human keratinocytes : a dose relevance study. Doctoral thesis, Nanyang Technological University, Singapore. https://hdl.handle.net/10356/65668 10.32657/10356/65668 en application/pdf |
| spellingShingle | DRNTU::Engineering::Nanotechnology Zhao, Yun Titanium dioxide and zinc oxide nanoparticles induce autophagy associated with toxicity effects in primary human keratinocytes : a dose relevance study |
| title | Titanium dioxide and zinc oxide nanoparticles induce autophagy associated with toxicity effects in primary human keratinocytes : a dose relevance study |
| title_full | Titanium dioxide and zinc oxide nanoparticles induce autophagy associated with toxicity effects in primary human keratinocytes : a dose relevance study |
| title_fullStr | Titanium dioxide and zinc oxide nanoparticles induce autophagy associated with toxicity effects in primary human keratinocytes : a dose relevance study |
| title_full_unstemmed | Titanium dioxide and zinc oxide nanoparticles induce autophagy associated with toxicity effects in primary human keratinocytes : a dose relevance study |
| title_short | Titanium dioxide and zinc oxide nanoparticles induce autophagy associated with toxicity effects in primary human keratinocytes : a dose relevance study |
| title_sort | titanium dioxide and zinc oxide nanoparticles induce autophagy associated with toxicity effects in primary human keratinocytes a dose relevance study |
| topic | DRNTU::Engineering::Nanotechnology |
| url | https://hdl.handle.net/10356/65668 |
| work_keys_str_mv | AT zhaoyun titaniumdioxideandzincoxidenanoparticlesinduceautophagyassociatedwithtoxicityeffectsinprimaryhumankeratinocytesadoserelevancestudy |