Molecular studies of the replication mechanisms of coronavirus
Coronavirus M protein is an essential component of virions and plays pivotal roles in virion assembly, budding and maturation. The M protein is integrated into the viral envelope with three transmembrane domains flanked by a short amino-terminal ectodomain and a large carboxy-terminal endodomain. To...
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| Format: | Thesis |
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2008
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| Online Access: | https://hdl.handle.net/10356/6574 |
| _version_ | 1826130289470996480 |
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| author | Wang, Jibin |
| author2 | James P. Tam |
| author_facet | James P. Tam Wang, Jibin |
| author_sort | Wang, Jibin |
| collection | NTU |
| description | Coronavirus M protein is an essential component of virions and plays pivotal roles in virion assembly, budding and maturation. The M protein is integrated into the viral envelope with three transmembrane domains flanked by a short amino-terminal ectodomain and a large carboxy-terminal endodomain. To understand the cellular factors that may be involved in virion assembly, budding and maturation processes, the M protein from coronavirus infectious bronchitis virus (IBV) was used as a bait in a yeast two-hybrid screen, resulting in the identification of -Actin as a potentially interacting partner. This interaction was subsequent confirmed by coimmunoprecipitation and immunofluorescence in mammalian cells, and mutation of amino acids A159 and K160 in the M protein abolished the interaction. |
| first_indexed | 2024-10-01T07:54:02Z |
| format | Thesis |
| id | ntu-10356/6574 |
| institution | Nanyang Technological University |
| last_indexed | 2024-10-01T07:54:02Z |
| publishDate | 2008 |
| record_format | dspace |
| spelling | ntu-10356/65742023-02-28T18:48:11Z Molecular studies of the replication mechanisms of coronavirus Wang, Jibin James P. Tam Liu Ding Xiang School of Biological Sciences DRNTU::Science::Biological sciences Coronavirus M protein is an essential component of virions and plays pivotal roles in virion assembly, budding and maturation. The M protein is integrated into the viral envelope with three transmembrane domains flanked by a short amino-terminal ectodomain and a large carboxy-terminal endodomain. To understand the cellular factors that may be involved in virion assembly, budding and maturation processes, the M protein from coronavirus infectious bronchitis virus (IBV) was used as a bait in a yeast two-hybrid screen, resulting in the identification of -Actin as a potentially interacting partner. This interaction was subsequent confirmed by coimmunoprecipitation and immunofluorescence in mammalian cells, and mutation of amino acids A159 and K160 in the M protein abolished the interaction. DOCTOR OF PHILOSOPHY (SBS) 2008-09-17T11:42:04Z 2008-09-17T11:42:04Z 2007 2007 Thesis Wang, J. B. (2007). Molecular studies of the replication mechanisms of coronavirus. Doctoral thesis, Nanyang Technological University, Singapore. https://hdl.handle.net/10356/6574 10.32657/10356/6574 Nanyang Technological University application/pdf |
| spellingShingle | DRNTU::Science::Biological sciences Wang, Jibin Molecular studies of the replication mechanisms of coronavirus |
| title | Molecular studies of the replication mechanisms of coronavirus |
| title_full | Molecular studies of the replication mechanisms of coronavirus |
| title_fullStr | Molecular studies of the replication mechanisms of coronavirus |
| title_full_unstemmed | Molecular studies of the replication mechanisms of coronavirus |
| title_short | Molecular studies of the replication mechanisms of coronavirus |
| title_sort | molecular studies of the replication mechanisms of coronavirus |
| topic | DRNTU::Science::Biological sciences |
| url | https://hdl.handle.net/10356/6574 |
| work_keys_str_mv | AT wangjibin molecularstudiesofthereplicationmechanismsofcoronavirus |