Preliminary study on the effect of high and low cyclic-di-GMP level on 3-chloroaniline biodegradation by Comamonas testosteroni
Several environmental pollutants, including biodegradable and non-biodegradable pollutants, under conditions of high enough concentrations can cause serious damage to the living organisms. Chloroanilines as nitrogen-containing aromatic compounds and one of xenobiotic pollutants are widely used chemi...
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| Other Authors: | |
| Format: | Final Year Project (FYP) |
| Language: | English |
| Published: |
2015
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| Online Access: | http://hdl.handle.net/10356/65768 |
| _version_ | 1824456503682138112 |
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| author | Qian, Duoduo |
| author2 | Cao Bin |
| author_facet | Cao Bin Qian, Duoduo |
| author_sort | Qian, Duoduo |
| collection | NTU |
| description | Several environmental pollutants, including biodegradable and non-biodegradable pollutants, under conditions of high enough concentrations can cause serious damage to the living organisms. Chloroanilines as nitrogen-containing aromatic compounds and one of xenobiotic pollutants are widely used chemicals in industry for the production of polyurethanes, rubber, azo dyes, drugs, and pesticides, in which 3-Chloroaniline is proved to have an inhibitive effect on bacterial growth. Comamonas testosteroni WDL7 were proved to have the ability of degrading 3-CA. C-di-GMP is a secondary messenger in bacteria determines bacterial life between the planktonic and biofilm life modes, which is synthesized by GGDEF domain proteins and degraded by EAL domain proteins. YedQ is a GGDEF domain protein and yhjH is of EAL domain, which can be mutated with pyedQ and pyhjH induction. Comamonas testosteroni WDL7 wild type, yedQ, and yhjH mutants were incubated with 3-cholroanilne as the sole carbon source in planktonic environment, in which mutant yedQ showed a better performance on growth and surviving, pyhjH mutant showed a delayed degradation profile. |
| first_indexed | 2025-02-19T03:55:08Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/65768 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2025-02-19T03:55:08Z |
| publishDate | 2015 |
| record_format | dspace |
| spelling | ntu-10356/657682023-03-03T17:10:55Z Preliminary study on the effect of high and low cyclic-di-GMP level on 3-chloroaniline biodegradation by Comamonas testosteroni Qian, Duoduo Cao Bin School of Civil and Environmental Engineering Singapore Centre for Environmental Life Sciences Engineering DRNTU::Engineering::Environmental engineering Several environmental pollutants, including biodegradable and non-biodegradable pollutants, under conditions of high enough concentrations can cause serious damage to the living organisms. Chloroanilines as nitrogen-containing aromatic compounds and one of xenobiotic pollutants are widely used chemicals in industry for the production of polyurethanes, rubber, azo dyes, drugs, and pesticides, in which 3-Chloroaniline is proved to have an inhibitive effect on bacterial growth. Comamonas testosteroni WDL7 were proved to have the ability of degrading 3-CA. C-di-GMP is a secondary messenger in bacteria determines bacterial life between the planktonic and biofilm life modes, which is synthesized by GGDEF domain proteins and degraded by EAL domain proteins. YedQ is a GGDEF domain protein and yhjH is of EAL domain, which can be mutated with pyedQ and pyhjH induction. Comamonas testosteroni WDL7 wild type, yedQ, and yhjH mutants were incubated with 3-cholroanilne as the sole carbon source in planktonic environment, in which mutant yedQ showed a better performance on growth and surviving, pyhjH mutant showed a delayed degradation profile. Bachelor of Engineering (Environmental Engineering) 2015-12-14T03:23:58Z 2015-12-14T03:23:58Z 2015 2015 Final Year Project (FYP) http://hdl.handle.net/10356/65768 en Nanyang Technological University 35 p. application/pdf |
| spellingShingle | DRNTU::Engineering::Environmental engineering Qian, Duoduo Preliminary study on the effect of high and low cyclic-di-GMP level on 3-chloroaniline biodegradation by Comamonas testosteroni |
| title | Preliminary study on the effect of high and low cyclic-di-GMP level on 3-chloroaniline biodegradation by Comamonas testosteroni |
| title_full | Preliminary study on the effect of high and low cyclic-di-GMP level on 3-chloroaniline biodegradation by Comamonas testosteroni |
| title_fullStr | Preliminary study on the effect of high and low cyclic-di-GMP level on 3-chloroaniline biodegradation by Comamonas testosteroni |
| title_full_unstemmed | Preliminary study on the effect of high and low cyclic-di-GMP level on 3-chloroaniline biodegradation by Comamonas testosteroni |
| title_short | Preliminary study on the effect of high and low cyclic-di-GMP level on 3-chloroaniline biodegradation by Comamonas testosteroni |
| title_sort | preliminary study on the effect of high and low cyclic di gmp level on 3 chloroaniline biodegradation by comamonas testosteroni |
| topic | DRNTU::Engineering::Environmental engineering |
| url | http://hdl.handle.net/10356/65768 |
| work_keys_str_mv | AT qianduoduo preliminarystudyontheeffectofhighandlowcyclicdigmplevelon3chloroanilinebiodegradationbycomamonastestosteroni |