Identification and development of novel antimicrobial therapies
My PhD thesis focuses on the identification and development of novel antimicrobial therapies against bacteria, with particular emphasis on addressing the biofilm mode of life and quorum sensing. In the first part of my thesis, I describe the use of whole genome sequencing to understand the developme...
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| Other Authors: | |
| Format: | Thesis |
| Language: | English |
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2015
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| Online Access: | https://hdl.handle.net/10356/65831 |
| _version_ | 1826111582896128000 |
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| author | Tan, Sean Yang-Yi |
| author2 | Michael Givskov |
| author_facet | Michael Givskov Tan, Sean Yang-Yi |
| author_sort | Tan, Sean Yang-Yi |
| collection | NTU |
| description | My PhD thesis focuses on the identification and development of novel antimicrobial therapies against bacteria, with particular emphasis on addressing the biofilm mode of life and quorum sensing. In the first part of my thesis, I describe the use of whole genome sequencing to understand the development of drug resistance in two Acinetobacter baumannii strains that were isolated from burn trauma victims hospitalized in an Intensive Care Unit in Rigshospitalet, Denmark.
In the second part of my thesis, I describe how I developed a fast, cost-effective and accurate in silico platform for the identification of novel quorum sensing inhibitors (QSIs) against the las QS system in Pseudomonas aeruginosa. Lastly, I describe how I used comparative systems biology tools (RNA-sequencing and proteomics) to shed light on a novel QS inhibition mechanism, the inhibition of small regulatory RNAs, by iberin, a natural compound isolated from horseradish. |
| first_indexed | 2024-10-01T02:53:08Z |
| format | Thesis |
| id | ntu-10356/65831 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T02:53:08Z |
| publishDate | 2015 |
| record_format | dspace |
| spelling | ntu-10356/658312023-02-28T18:32:43Z Identification and development of novel antimicrobial therapies Tan, Sean Yang-Yi Michael Givskov School of Biological Sciences Singapore Centre for Environmental Life Sciences Engineering DRNTU::Science::Biological sciences::Microbiology::Drug Resistance My PhD thesis focuses on the identification and development of novel antimicrobial therapies against bacteria, with particular emphasis on addressing the biofilm mode of life and quorum sensing. In the first part of my thesis, I describe the use of whole genome sequencing to understand the development of drug resistance in two Acinetobacter baumannii strains that were isolated from burn trauma victims hospitalized in an Intensive Care Unit in Rigshospitalet, Denmark. In the second part of my thesis, I describe how I developed a fast, cost-effective and accurate in silico platform for the identification of novel quorum sensing inhibitors (QSIs) against the las QS system in Pseudomonas aeruginosa. Lastly, I describe how I used comparative systems biology tools (RNA-sequencing and proteomics) to shed light on a novel QS inhibition mechanism, the inhibition of small regulatory RNAs, by iberin, a natural compound isolated from horseradish. DOCTOR OF PHILOSOPHY (SBS) 2015-12-22T01:14:09Z 2015-12-22T01:14:09Z 2015 2015 Thesis Tan, S. Y.-Y. (2015). Identification and development of novel antimicrobial therapies. Doctoral thesis, Nanyang Technological University, Singapore. https://hdl.handle.net/10356/65831 10.32657/10356/65831 en 159 p. application/pdf |
| spellingShingle | DRNTU::Science::Biological sciences::Microbiology::Drug Resistance Tan, Sean Yang-Yi Identification and development of novel antimicrobial therapies |
| title | Identification and development of novel antimicrobial therapies |
| title_full | Identification and development of novel antimicrobial therapies |
| title_fullStr | Identification and development of novel antimicrobial therapies |
| title_full_unstemmed | Identification and development of novel antimicrobial therapies |
| title_short | Identification and development of novel antimicrobial therapies |
| title_sort | identification and development of novel antimicrobial therapies |
| topic | DRNTU::Science::Biological sciences::Microbiology::Drug Resistance |
| url | https://hdl.handle.net/10356/65831 |
| work_keys_str_mv | AT tanseanyangyi identificationanddevelopmentofnovelantimicrobialtherapies |