The role of autophagy in maintenance of genomic integrity
Autophagy is an intracellular degradation pathway that is important for sustaining cellular metabolism. It is a catabolic process whereby cytoplasmic components such as damaged proteins and organelles are delivered to the lysosomes for degradation. Beyond autophagy’s housekeeping functions, recent s...
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| Format: | Thesis |
| Language: | English |
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2016
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| Online Access: | https://hdl.handle.net/10356/66007 |
| _version_ | 1826120860037021696 |
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| author | Zhang, Yu Cui |
| author2 | Wong Siew Peng Esther |
| author_facet | Wong Siew Peng Esther Zhang, Yu Cui |
| author_sort | Zhang, Yu Cui |
| collection | NTU |
| description | Autophagy is an intracellular degradation pathway that is important for sustaining cellular metabolism. It is a catabolic process whereby cytoplasmic components such as damaged proteins and organelles are delivered to the lysosomes for degradation. Beyond autophagy’s housekeeping functions, recent studies have uncovered a myriad of physiological and pathophysiological roles played by autophagy, such as the modulation of host defenses, ageing and tumorigenesis. In particular, my interest lies in autophagy’s role in maintenance of genomic integrity. However, the mechanisms underlying this role remain elusive. Here, autophagy inhibition resulted in reduced protein levels of condensin-II and Kif4a. This negatively affected the amount of condensin-II and Kif4a localized on the chromosomes and in turn, impeded proper chromosome condensation and kinetochore-microtubule attachments during mitosis. As a result, there was increased incidence of lagging chromosomes from aberrant chromosome segregation, thus compromising genomic integrity. These indicated a pathway where autophagy enables proper chromosome segregation through its regulation on the protein levels of condensin-II and Kif4a. |
| first_indexed | 2024-10-01T05:23:21Z |
| format | Thesis |
| id | ntu-10356/66007 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T05:23:21Z |
| publishDate | 2016 |
| record_format | dspace |
| spelling | ntu-10356/660072023-02-28T18:40:23Z The role of autophagy in maintenance of genomic integrity Zhang, Yu Cui Wong Siew Peng Esther Li Hoi Yeung School of Biological Sciences DRNTU::Science::Biological sciences Autophagy is an intracellular degradation pathway that is important for sustaining cellular metabolism. It is a catabolic process whereby cytoplasmic components such as damaged proteins and organelles are delivered to the lysosomes for degradation. Beyond autophagy’s housekeeping functions, recent studies have uncovered a myriad of physiological and pathophysiological roles played by autophagy, such as the modulation of host defenses, ageing and tumorigenesis. In particular, my interest lies in autophagy’s role in maintenance of genomic integrity. However, the mechanisms underlying this role remain elusive. Here, autophagy inhibition resulted in reduced protein levels of condensin-II and Kif4a. This negatively affected the amount of condensin-II and Kif4a localized on the chromosomes and in turn, impeded proper chromosome condensation and kinetochore-microtubule attachments during mitosis. As a result, there was increased incidence of lagging chromosomes from aberrant chromosome segregation, thus compromising genomic integrity. These indicated a pathway where autophagy enables proper chromosome segregation through its regulation on the protein levels of condensin-II and Kif4a. DOCTOR OF PHILOSOPHY (SBS) 2016-02-29T01:35:20Z 2016-02-29T01:35:20Z 2016 Thesis Zhang, Y. C. (2016). The role of autophagy in maintenance of genomic integrity. Doctoral thesis, Nanyang Technological University, Singapore. https://hdl.handle.net/10356/66007 10.32657/10356/66007 en 112 p. application/pdf |
| spellingShingle | DRNTU::Science::Biological sciences Zhang, Yu Cui The role of autophagy in maintenance of genomic integrity |
| title | The role of autophagy in maintenance of genomic integrity |
| title_full | The role of autophagy in maintenance of genomic integrity |
| title_fullStr | The role of autophagy in maintenance of genomic integrity |
| title_full_unstemmed | The role of autophagy in maintenance of genomic integrity |
| title_short | The role of autophagy in maintenance of genomic integrity |
| title_sort | role of autophagy in maintenance of genomic integrity |
| topic | DRNTU::Science::Biological sciences |
| url | https://hdl.handle.net/10356/66007 |
| work_keys_str_mv | AT zhangyucui theroleofautophagyinmaintenanceofgenomicintegrity AT zhangyucui roleofautophagyinmaintenanceofgenomicintegrity |