Osmogen mediated hollow microparticles fabrication for peptide encapsulation and delivery
Bioactive molecules such as peptides and proteins have significant therapeutic potential. However, their therapeutic ability is limited by their low systemic stability, short half-life, and subsequent loss of bioactivity. In order to increase their stability, drug efficacy, and to achieve a sustaine...
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| Other Authors: | |
| Format: | Final Year Project (FYP) |
| Language: | English |
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2016
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| Online Access: | http://hdl.handle.net/10356/66345 |
| _version_ | 1811687174400114688 |
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| author | Lee, Xuan Yi |
| author2 | Loo Say Chye Joachim |
| author_facet | Loo Say Chye Joachim Lee, Xuan Yi |
| author_sort | Lee, Xuan Yi |
| collection | NTU |
| description | Bioactive molecules such as peptides and proteins have significant therapeutic potential. However, their therapeutic ability is limited by their low systemic stability, short half-life, and subsequent loss of bioactivity. In order to increase their stability, drug efficacy, and to achieve a sustained release profile, encapsulation with poly(lactic-co-glycolic acid) (PLGA), is carried out.
PLGA hollow microparticles were fabricated by a novel osmogen mediated solvent emulsion evaporation technique with the aim of minimizing the creation of a localized acidic microenvironment, which causes peptide denaturation. The effects of various process parameters on the morphology of the hollow microparticles were studied and characterized by scanning electron microscope (SEM). Thereafter, these hollow microparticles were loaded with the peptide, Glucagon-like peptide-1 (GLP-1), and its subsequent encapsulation efficiency and in vitro release behaviour were analyzed. |
| first_indexed | 2024-10-01T05:12:07Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/66345 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T05:12:07Z |
| publishDate | 2016 |
| record_format | dspace |
| spelling | ntu-10356/663452023-03-04T15:42:10Z Osmogen mediated hollow microparticles fabrication for peptide encapsulation and delivery Lee, Xuan Yi Loo Say Chye Joachim School of Materials Science and Engineering DRNTU::Engineering Bioactive molecules such as peptides and proteins have significant therapeutic potential. However, their therapeutic ability is limited by their low systemic stability, short half-life, and subsequent loss of bioactivity. In order to increase their stability, drug efficacy, and to achieve a sustained release profile, encapsulation with poly(lactic-co-glycolic acid) (PLGA), is carried out. PLGA hollow microparticles were fabricated by a novel osmogen mediated solvent emulsion evaporation technique with the aim of minimizing the creation of a localized acidic microenvironment, which causes peptide denaturation. The effects of various process parameters on the morphology of the hollow microparticles were studied and characterized by scanning electron microscope (SEM). Thereafter, these hollow microparticles were loaded with the peptide, Glucagon-like peptide-1 (GLP-1), and its subsequent encapsulation efficiency and in vitro release behaviour were analyzed. Bachelor of Engineering (Materials Engineering) 2016-03-30T01:45:32Z 2016-03-30T01:45:32Z 2016 Final Year Project (FYP) http://hdl.handle.net/10356/66345 en Nanyang Technological University 33 p. application/pdf |
| spellingShingle | DRNTU::Engineering Lee, Xuan Yi Osmogen mediated hollow microparticles fabrication for peptide encapsulation and delivery |
| title | Osmogen mediated hollow microparticles fabrication for peptide encapsulation and delivery |
| title_full | Osmogen mediated hollow microparticles fabrication for peptide encapsulation and delivery |
| title_fullStr | Osmogen mediated hollow microparticles fabrication for peptide encapsulation and delivery |
| title_full_unstemmed | Osmogen mediated hollow microparticles fabrication for peptide encapsulation and delivery |
| title_short | Osmogen mediated hollow microparticles fabrication for peptide encapsulation and delivery |
| title_sort | osmogen mediated hollow microparticles fabrication for peptide encapsulation and delivery |
| topic | DRNTU::Engineering |
| url | http://hdl.handle.net/10356/66345 |
| work_keys_str_mv | AT leexuanyi osmogenmediatedhollowmicroparticlesfabricationforpeptideencapsulationanddelivery |