Microfluidics size fractionation of paclitaxel-loaded microspheres for breast cancer treatment
Drug-loaded microspheres are widely used in cancer treatment through targeted and sustained drug delivery. In this study, poly(lactic-co-glycolic acid) (PLGA) microspheres loaded with paclitaxel (PTX) are synthesized and size-fractionated using a novel inertial microfluidics strategy (Dean Flow Frac...
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| Format: | Final Year Project (FYP) |
| Language: | English |
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2016
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| Online Access: | http://hdl.handle.net/10356/66444 |
| _version_ | 1811687012242030592 |
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| author | Tan, Kah Kee |
| author2 | Loo Say Chye Joachim |
| author_facet | Loo Say Chye Joachim Tan, Kah Kee |
| author_sort | Tan, Kah Kee |
| collection | NTU |
| description | Drug-loaded microspheres are widely used in cancer treatment through targeted and sustained drug delivery. In this study, poly(lactic-co-glycolic acid) (PLGA) microspheres loaded with paclitaxel (PTX) are synthesized and size-fractionated using a novel inertial microfluidics strategy (Dean Flow Fractionation) for enhanced inhibition of breast cancer cell proliferation. The polydimethylsiloxane (PDMS) microdevice was characterized by optical imaging methods (high speed and fluorescence) and flow cytometry using fluorescent microbeads. After device optimization, PTX-loaded microparticles were fractionated into 3 different size groups (>5µm, 3-5µm and <3µm) and co-incubated with MCF-7 breast cancer cells. CCK Proliferation (CCK) assay and imaging showed that smaller microparticles (<3µm) were more effective at inhibiting cell proliferation. The developed technology provides a novel high throughput sample preparation method for nanoparticle synthesis and drug delivery systems. |
| first_indexed | 2024-10-01T05:09:32Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/66444 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T05:09:32Z |
| publishDate | 2016 |
| record_format | dspace |
| spelling | ntu-10356/664442023-03-04T15:41:13Z Microfluidics size fractionation of paclitaxel-loaded microspheres for breast cancer treatment Tan, Kah Kee Loo Say Chye Joachim School of Materials Science and Engineering DRNTU::Engineering Drug-loaded microspheres are widely used in cancer treatment through targeted and sustained drug delivery. In this study, poly(lactic-co-glycolic acid) (PLGA) microspheres loaded with paclitaxel (PTX) are synthesized and size-fractionated using a novel inertial microfluidics strategy (Dean Flow Fractionation) for enhanced inhibition of breast cancer cell proliferation. The polydimethylsiloxane (PDMS) microdevice was characterized by optical imaging methods (high speed and fluorescence) and flow cytometry using fluorescent microbeads. After device optimization, PTX-loaded microparticles were fractionated into 3 different size groups (>5µm, 3-5µm and <3µm) and co-incubated with MCF-7 breast cancer cells. CCK Proliferation (CCK) assay and imaging showed that smaller microparticles (<3µm) were more effective at inhibiting cell proliferation. The developed technology provides a novel high throughput sample preparation method for nanoparticle synthesis and drug delivery systems. Bachelor of Engineering (Materials Engineering) 2016-04-07T04:45:04Z 2016-04-07T04:45:04Z 2016 Final Year Project (FYP) http://hdl.handle.net/10356/66444 en Nanyang Technological University 36 p. application/pdf |
| spellingShingle | DRNTU::Engineering Tan, Kah Kee Microfluidics size fractionation of paclitaxel-loaded microspheres for breast cancer treatment |
| title | Microfluidics size fractionation of paclitaxel-loaded microspheres for breast cancer treatment |
| title_full | Microfluidics size fractionation of paclitaxel-loaded microspheres for breast cancer treatment |
| title_fullStr | Microfluidics size fractionation of paclitaxel-loaded microspheres for breast cancer treatment |
| title_full_unstemmed | Microfluidics size fractionation of paclitaxel-loaded microspheres for breast cancer treatment |
| title_short | Microfluidics size fractionation of paclitaxel-loaded microspheres for breast cancer treatment |
| title_sort | microfluidics size fractionation of paclitaxel loaded microspheres for breast cancer treatment |
| topic | DRNTU::Engineering |
| url | http://hdl.handle.net/10356/66444 |
| work_keys_str_mv | AT tankahkee microfluidicssizefractionationofpaclitaxelloadedmicrospheresforbreastcancertreatment |