The role of transportin-1 in the ciliary targeting of membrane proteins
The sensory functions of cilia are dependent on the enrichment of ciliary resident proteins. While it is known that ciliary targeting signals (CTSs) specifically target ciliary proteins to cilia, it is less clear how CTSs facilitate the entry and retention of ciliary residents at molecular level. We...
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| Other Authors: | |
| Format: | Thesis |
| Language: | English |
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2016
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| Online Access: | https://hdl.handle.net/10356/67319 |
| _version_ | 1824454538430513152 |
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| author | Madugula, Viswanadh |
| author2 | Lu Lei |
| author_facet | Lu Lei Madugula, Viswanadh |
| author_sort | Madugula, Viswanadh |
| collection | NTU |
| description | The sensory functions of cilia are dependent on the enrichment of ciliary resident proteins. While it is known that ciliary targeting signals (CTSs) specifically target ciliary proteins to cilia, it is less clear how CTSs facilitate the entry and retention of ciliary residents at molecular level. We found that non-ciliary membrane reporters could passively diffuse to cilia via the lateral transport pathway and the translocation of membrane reporters through ciliary diffusion barrier could be facilitated by importin binding motifs/domains. We further discovered that a novel ternary complex, comprising Rab8, TNPO1 and CTS, could be assembled or disassembled under the guanine nucleotide exchange of Rab8. Our study suggests a novel mechanism in which Rab8/TNPO1/CTS complex mediates selective entry and retention of cargos within cilia. |
| first_indexed | 2025-02-19T03:23:54Z |
| format | Thesis |
| id | ntu-10356/67319 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2025-02-19T03:23:54Z |
| publishDate | 2016 |
| record_format | dspace |
| spelling | ntu-10356/673192023-02-28T18:49:29Z The role of transportin-1 in the ciliary targeting of membrane proteins Madugula, Viswanadh Lu Lei School of Biological Sciences DRNTU::Science::Biological sciences The sensory functions of cilia are dependent on the enrichment of ciliary resident proteins. While it is known that ciliary targeting signals (CTSs) specifically target ciliary proteins to cilia, it is less clear how CTSs facilitate the entry and retention of ciliary residents at molecular level. We found that non-ciliary membrane reporters could passively diffuse to cilia via the lateral transport pathway and the translocation of membrane reporters through ciliary diffusion barrier could be facilitated by importin binding motifs/domains. We further discovered that a novel ternary complex, comprising Rab8, TNPO1 and CTS, could be assembled or disassembled under the guanine nucleotide exchange of Rab8. Our study suggests a novel mechanism in which Rab8/TNPO1/CTS complex mediates selective entry and retention of cargos within cilia. DOCTOR OF PHILOSOPHY (SBS) 2016-05-15T08:24:59Z 2016-05-15T08:24:59Z 2016 Thesis Madugula, V. (2016). The role of transportin-1 in the ciliary targeting of membrane proteins. Doctoral thesis, Nanyang Technological University, Singapore. https://hdl.handle.net/10356/67319 10.32657/10356/67319 en 129 p. application/pdf |
| spellingShingle | DRNTU::Science::Biological sciences Madugula, Viswanadh The role of transportin-1 in the ciliary targeting of membrane proteins |
| title | The role of transportin-1 in the ciliary targeting of membrane proteins |
| title_full | The role of transportin-1 in the ciliary targeting of membrane proteins |
| title_fullStr | The role of transportin-1 in the ciliary targeting of membrane proteins |
| title_full_unstemmed | The role of transportin-1 in the ciliary targeting of membrane proteins |
| title_short | The role of transportin-1 in the ciliary targeting of membrane proteins |
| title_sort | role of transportin 1 in the ciliary targeting of membrane proteins |
| topic | DRNTU::Science::Biological sciences |
| url | https://hdl.handle.net/10356/67319 |
| work_keys_str_mv | AT madugulaviswanadh theroleoftransportin1intheciliarytargetingofmembraneproteins AT madugulaviswanadh roleoftransportin1intheciliarytargetingofmembraneproteins |