Enhancement of mesenchymal stem cells (MSCs) adhesion, proliferation and long-term cell growth on PDMS surfaces : the interplay between different substratum physical properties
Polydimethylsiloxane (PDMS) is a popular biomaterial used for microscale-cell analysis in microfluidic chips as a result of its numerous advantages such as low toxicity level, ease of modification and biocompatibility. However, the innate hydrophobicity of PDMS poses a problem for cell binding on...
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| Format: | Final Year Project (FYP) |
| Language: | English |
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2016
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| Online Access: | http://hdl.handle.net/10356/68099 |
| _version_ | 1811686157702922240 |
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| author | Heng, Zhi Ting |
| author2 | Wang Dongan |
| author_facet | Wang Dongan Heng, Zhi Ting |
| author_sort | Heng, Zhi Ting |
| collection | NTU |
| description | Polydimethylsiloxane (PDMS) is a popular biomaterial used for microscale-cell
analysis in microfluidic chips as a result of its numerous advantages such as low toxicity
level, ease of modification and biocompatibility. However, the innate hydrophobicity of
PDMS poses a problem for cell binding onto its substrates and surface modification on
PDMS would be necessary to enhance the attachment of cells and subsequent cell
growth. This study explores the effect of plasma treatment on PDMS in the application of
BMSC culture as plasma treatment is a relatively simple and cost-effective surface
modification technique. In addition, PDMS substrates of different prepolymer base to
curing agent ratio were fabricated to examine the influence of physical substratum
properties on BMSC behavior. Results from this study have shown that plasma treatment
on PDMS surface can significantly promote BMSC adhesion and cell proliferation rate,
thereby, enabling a stable and confluent cell sheet formation. Surface characterization
analysis has also revealed a variation in the physical substratum properties of plasma
treated PDMS substrates, where a higher surface roughness and a low water contact angle
were observed. This suggests that physical substratum properties can influence cell
behavior. PDMS substrates of various base to curing agent ratio also exhibit different
substratum properties, however, there are no significant improvement in the long-term
cell growth. Findings in this study may be useful for future research in the fabrication of
biomaterials and application of BMSC cell growth. |
| first_indexed | 2024-10-01T04:55:57Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/68099 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:55:57Z |
| publishDate | 2016 |
| record_format | dspace |
| spelling | ntu-10356/680992023-03-03T15:35:35Z Enhancement of mesenchymal stem cells (MSCs) adhesion, proliferation and long-term cell growth on PDMS surfaces : the interplay between different substratum physical properties Heng, Zhi Ting Wang Dongan School of Chemical and Biomedical Engineering Kang YueJun DRNTU::Engineering Polydimethylsiloxane (PDMS) is a popular biomaterial used for microscale-cell analysis in microfluidic chips as a result of its numerous advantages such as low toxicity level, ease of modification and biocompatibility. However, the innate hydrophobicity of PDMS poses a problem for cell binding onto its substrates and surface modification on PDMS would be necessary to enhance the attachment of cells and subsequent cell growth. This study explores the effect of plasma treatment on PDMS in the application of BMSC culture as plasma treatment is a relatively simple and cost-effective surface modification technique. In addition, PDMS substrates of different prepolymer base to curing agent ratio were fabricated to examine the influence of physical substratum properties on BMSC behavior. Results from this study have shown that plasma treatment on PDMS surface can significantly promote BMSC adhesion and cell proliferation rate, thereby, enabling a stable and confluent cell sheet formation. Surface characterization analysis has also revealed a variation in the physical substratum properties of plasma treated PDMS substrates, where a higher surface roughness and a low water contact angle were observed. This suggests that physical substratum properties can influence cell behavior. PDMS substrates of various base to curing agent ratio also exhibit different substratum properties, however, there are no significant improvement in the long-term cell growth. Findings in this study may be useful for future research in the fabrication of biomaterials and application of BMSC cell growth. Bachelor of Engineering (Chemical and Biomolecular Engineering) 2016-05-24T05:37:43Z 2016-05-24T05:37:43Z 2016 Final Year Project (FYP) http://hdl.handle.net/10356/68099 en Nanyang Technological University 41 p. application/pdf |
| spellingShingle | DRNTU::Engineering Heng, Zhi Ting Enhancement of mesenchymal stem cells (MSCs) adhesion, proliferation and long-term cell growth on PDMS surfaces : the interplay between different substratum physical properties |
| title | Enhancement of mesenchymal stem cells (MSCs) adhesion, proliferation and long-term cell growth on PDMS surfaces : the interplay between different substratum physical properties |
| title_full | Enhancement of mesenchymal stem cells (MSCs) adhesion, proliferation and long-term cell growth on PDMS surfaces : the interplay between different substratum physical properties |
| title_fullStr | Enhancement of mesenchymal stem cells (MSCs) adhesion, proliferation and long-term cell growth on PDMS surfaces : the interplay between different substratum physical properties |
| title_full_unstemmed | Enhancement of mesenchymal stem cells (MSCs) adhesion, proliferation and long-term cell growth on PDMS surfaces : the interplay between different substratum physical properties |
| title_short | Enhancement of mesenchymal stem cells (MSCs) adhesion, proliferation and long-term cell growth on PDMS surfaces : the interplay between different substratum physical properties |
| title_sort | enhancement of mesenchymal stem cells mscs adhesion proliferation and long term cell growth on pdms surfaces the interplay between different substratum physical properties |
| topic | DRNTU::Engineering |
| url | http://hdl.handle.net/10356/68099 |
| work_keys_str_mv | AT hengzhiting enhancementofmesenchymalstemcellsmscsadhesionproliferationandlongtermcellgrowthonpdmssurfacestheinterplaybetweendifferentsubstratumphysicalproperties |