Modeling engineered microbes that uses renewable resource as feedstock to produce useful products
Computational tools can be used to accurately represent the behaviour of complex biological pathways. This report describes the computational model chosen, specific to Vanillin production pathways and describes how these pathways can be modelled using a “top-down” approach relating to its metabolite...
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| Format: | Final Year Project (FYP) |
| Language: | English |
| Published: |
2016
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| Online Access: | http://hdl.handle.net/10356/68455 |
| _version_ | 1811684136048394240 |
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| author | Lau, Andy Ying Cheng |
| author2 | Poh Chueh Loo |
| author_facet | Poh Chueh Loo Lau, Andy Ying Cheng |
| author_sort | Lau, Andy Ying Cheng |
| collection | NTU |
| description | Computational tools can be used to accurately represent the behaviour of complex biological pathways. This report describes the computational model chosen, specific to Vanillin production pathways and describes how these pathways can be modelled using a “top-down” approach relating to its metabolites. Furthermore, a simulation on the production of Vanillin is produced to predict the concentrations of each involving metabolites within the pathways. This is to done to accurately analysis the production pathway of Vanillin with ferulic acid being its source.
The simulation of this vanillin production pathway was further investigated. After running different simulations, the sensitivity of each enzyme, trans-feruloyl-CoA and synthase enoyl-CoA hydratase, in this pathway were analysed. Trans-feruloyl-CoA was determined to have a higher sensitivity than synthase enoyl-CoA hydratase. This analysis enables further researchers to alter the concentration of trans-feruloyl-CoA enzyme production to increase the overall vanillin production.
Overall, this computer model can be used as a tool to guide wet bench based experiments. This successfully accelerates the biological research and simplifies the biological pathways to be worked on. |
| first_indexed | 2024-10-01T04:23:49Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/68455 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:23:49Z |
| publishDate | 2016 |
| record_format | dspace |
| spelling | ntu-10356/684552023-03-03T15:34:54Z Modeling engineered microbes that uses renewable resource as feedstock to produce useful products Lau, Andy Ying Cheng Poh Chueh Loo School of Chemical and Biomedical Engineering DRNTU::Engineering Computational tools can be used to accurately represent the behaviour of complex biological pathways. This report describes the computational model chosen, specific to Vanillin production pathways and describes how these pathways can be modelled using a “top-down” approach relating to its metabolites. Furthermore, a simulation on the production of Vanillin is produced to predict the concentrations of each involving metabolites within the pathways. This is to done to accurately analysis the production pathway of Vanillin with ferulic acid being its source. The simulation of this vanillin production pathway was further investigated. After running different simulations, the sensitivity of each enzyme, trans-feruloyl-CoA and synthase enoyl-CoA hydratase, in this pathway were analysed. Trans-feruloyl-CoA was determined to have a higher sensitivity than synthase enoyl-CoA hydratase. This analysis enables further researchers to alter the concentration of trans-feruloyl-CoA enzyme production to increase the overall vanillin production. Overall, this computer model can be used as a tool to guide wet bench based experiments. This successfully accelerates the biological research and simplifies the biological pathways to be worked on. Bachelor of Engineering (Chemical and Biomolecular Engineering) 2016-05-26T03:06:04Z 2016-05-26T03:06:04Z 2016 Final Year Project (FYP) http://hdl.handle.net/10356/68455 en Nanyang Technological University 67 p. application/pdf |
| spellingShingle | DRNTU::Engineering Lau, Andy Ying Cheng Modeling engineered microbes that uses renewable resource as feedstock to produce useful products |
| title | Modeling engineered microbes that uses renewable resource as feedstock to produce useful products |
| title_full | Modeling engineered microbes that uses renewable resource as feedstock to produce useful products |
| title_fullStr | Modeling engineered microbes that uses renewable resource as feedstock to produce useful products |
| title_full_unstemmed | Modeling engineered microbes that uses renewable resource as feedstock to produce useful products |
| title_short | Modeling engineered microbes that uses renewable resource as feedstock to produce useful products |
| title_sort | modeling engineered microbes that uses renewable resource as feedstock to produce useful products |
| topic | DRNTU::Engineering |
| url | http://hdl.handle.net/10356/68455 |
| work_keys_str_mv | AT lauandyyingcheng modelingengineeredmicrobesthatusesrenewableresourceasfeedstocktoproduceusefulproducts |