Algae salinity tolerance enhancement mediated by interaction with bacteria
This study investigates the mutualistic relationship between Chlamydomonas reinhardtii and Comamonas testosteroni I2 when cultured in 0.7% salinity TP media. When subjected to abiotic stresses, algal-bacterial co-culture yielded better growth compared to algal monoculture, a result which was observe...
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| Other Authors: | |
| Format: | Final Year Project (FYP) |
| Language: | English |
| Published: |
2017
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| Online Access: | http://hdl.handle.net/10356/70855 |
| _version_ | 1826127011286876160 |
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| author | Ng, Cheng Yee |
| author2 | Cao Bin |
| author_facet | Cao Bin Ng, Cheng Yee |
| author_sort | Ng, Cheng Yee |
| collection | NTU |
| description | This study investigates the mutualistic relationship between Chlamydomonas reinhardtii and Comamonas testosteroni I2 when cultured in 0.7% salinity TP media. When subjected to abiotic stresses, algal-bacterial co-culture yielded better growth compared to algal monoculture, a result which was observed across three bacterial strains. Specimens in saline media displayed higher chlorophyll concentrations in co-culture than in monoculture, and when cultivated in an environment of high surface area to volume ratio than in liquid suspension. Analysis of supernatant samples extracted and filtered from 96-well microplate samples indicated the production of organic carbon by alga, implicating cross-feeding between algae and vitamin B12-producing I2. Cell imaging provided visual evidence of bacterial biofilm formation surrounding algae cells. |
| first_indexed | 2024-10-01T07:02:05Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/70855 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T07:02:05Z |
| publishDate | 2017 |
| record_format | dspace |
| spelling | ntu-10356/708552023-03-03T16:52:25Z Algae salinity tolerance enhancement mediated by interaction with bacteria Ng, Cheng Yee Cao Bin School of Civil and Environmental Engineering Singapore Centre for Environmental Life Sciences Engineering DRNTU::Engineering::Environmental engineering DRNTU::Science::Biological sciences::Microbiology::Microorganisms This study investigates the mutualistic relationship between Chlamydomonas reinhardtii and Comamonas testosteroni I2 when cultured in 0.7% salinity TP media. When subjected to abiotic stresses, algal-bacterial co-culture yielded better growth compared to algal monoculture, a result which was observed across three bacterial strains. Specimens in saline media displayed higher chlorophyll concentrations in co-culture than in monoculture, and when cultivated in an environment of high surface area to volume ratio than in liquid suspension. Analysis of supernatant samples extracted and filtered from 96-well microplate samples indicated the production of organic carbon by alga, implicating cross-feeding between algae and vitamin B12-producing I2. Cell imaging provided visual evidence of bacterial biofilm formation surrounding algae cells. Bachelor of Engineering (Environmental Engineering) 2017-05-11T08:55:15Z 2017-05-11T08:55:15Z 2017 Final Year Project (FYP) http://hdl.handle.net/10356/70855 en Nanyang Technological University 40 p. application/pdf |
| spellingShingle | DRNTU::Engineering::Environmental engineering DRNTU::Science::Biological sciences::Microbiology::Microorganisms Ng, Cheng Yee Algae salinity tolerance enhancement mediated by interaction with bacteria |
| title | Algae salinity tolerance enhancement mediated by interaction with bacteria |
| title_full | Algae salinity tolerance enhancement mediated by interaction with bacteria |
| title_fullStr | Algae salinity tolerance enhancement mediated by interaction with bacteria |
| title_full_unstemmed | Algae salinity tolerance enhancement mediated by interaction with bacteria |
| title_short | Algae salinity tolerance enhancement mediated by interaction with bacteria |
| title_sort | algae salinity tolerance enhancement mediated by interaction with bacteria |
| topic | DRNTU::Engineering::Environmental engineering DRNTU::Science::Biological sciences::Microbiology::Microorganisms |
| url | http://hdl.handle.net/10356/70855 |
| work_keys_str_mv | AT ngchengyee algaesalinitytoleranceenhancementmediatedbyinteractionwithbacteria |