Attacking stressed Pseudomonas protegen biofilms with Wi-Fi
Pseudomonas protegens (Pf-5) is a plant-based, gram-negative bacteria that can be readily found in various surrounding environments; from the natural-occurring environments to the man-made industrial environments. Inoculums were diluted at an absorbance of 0.05 to obtain measurements of 1×108 CFU/ml...
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| Format: | Final Year Project (FYP) |
| Language: | English |
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2017
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| Online Access: | http://hdl.handle.net/10356/72089 |
| _version_ | 1826129071845670912 |
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| author | Najmah Abdul Razak |
| author2 | Christopher Shearwood |
| author_facet | Christopher Shearwood Najmah Abdul Razak |
| author_sort | Najmah Abdul Razak |
| collection | NTU |
| description | Pseudomonas protegens (Pf-5) is a plant-based, gram-negative bacteria that can be readily found in various surrounding environments; from the natural-occurring environments to the man-made industrial environments. Inoculums were diluted at an absorbance of 0.05 to obtain measurements of 1×108 CFU/ml at a wavelength of 670 nm. The biofilms of the Pseudomonas protegens were cultured in 96-polystyrene microplates at 25±0.1°C with an agitation of 100 rpm in M9 minimal media at 10 varying concentrations of MgCl2 (0 Mm/L, 0.0145 Mm/L, 0.0291 Mm/L, 0.058 Mm/L, 0.116 Mm/L, 0.23 Mm/L, 0.465 Mm/L, 0.93 Mm/L, 2 Mm/L and 3.72 mM/L).
There were two 20-hour cycles of incubation. The first cycle is an incubation period to grow the biofilms under different concentrations of magnesium ions (Mg2+) without the influence of Wi-Fi. The second 20- hour cycle sees the biofilms being subjected to Wi-Fi signals. Control experiments were conducted as well to compare differences between the effects of biofilm formation and behaviour when subjected to Wi-Fi and when not subjected to Wi-Fi. Findings have shown that differences in nutrient concentrations are significant. Higher Mg2+ nutrient availability resulted in higher absorbance measured of the growth of biofilms as compared to lower Mg2+ nutrient availability. There were no significant effects of Wi-Fi on biofilm growth. However, Wi-Fi may have enhanced the formation of biofilms, and results were supported by correlation coefficients obtained. Correlation coefficients were higher when biofilms were subjected to Wi-Fi as compared to those that were not subjected to Wi-Fi. |
| first_indexed | 2024-10-01T07:34:44Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/72089 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T07:34:44Z |
| publishDate | 2017 |
| record_format | dspace |
| spelling | ntu-10356/720892023-03-04T19:19:03Z Attacking stressed Pseudomonas protegen biofilms with Wi-Fi Najmah Abdul Razak Christopher Shearwood School of Mechanical and Aerospace Engineering DRNTU::Engineering::Mechanical engineering Pseudomonas protegens (Pf-5) is a plant-based, gram-negative bacteria that can be readily found in various surrounding environments; from the natural-occurring environments to the man-made industrial environments. Inoculums were diluted at an absorbance of 0.05 to obtain measurements of 1×108 CFU/ml at a wavelength of 670 nm. The biofilms of the Pseudomonas protegens were cultured in 96-polystyrene microplates at 25±0.1°C with an agitation of 100 rpm in M9 minimal media at 10 varying concentrations of MgCl2 (0 Mm/L, 0.0145 Mm/L, 0.0291 Mm/L, 0.058 Mm/L, 0.116 Mm/L, 0.23 Mm/L, 0.465 Mm/L, 0.93 Mm/L, 2 Mm/L and 3.72 mM/L). There were two 20-hour cycles of incubation. The first cycle is an incubation period to grow the biofilms under different concentrations of magnesium ions (Mg2+) without the influence of Wi-Fi. The second 20- hour cycle sees the biofilms being subjected to Wi-Fi signals. Control experiments were conducted as well to compare differences between the effects of biofilm formation and behaviour when subjected to Wi-Fi and when not subjected to Wi-Fi. Findings have shown that differences in nutrient concentrations are significant. Higher Mg2+ nutrient availability resulted in higher absorbance measured of the growth of biofilms as compared to lower Mg2+ nutrient availability. There were no significant effects of Wi-Fi on biofilm growth. However, Wi-Fi may have enhanced the formation of biofilms, and results were supported by correlation coefficients obtained. Correlation coefficients were higher when biofilms were subjected to Wi-Fi as compared to those that were not subjected to Wi-Fi. Bachelor of Engineering (Mechanical Engineering) 2017-05-25T03:17:02Z 2017-05-25T03:17:02Z 2017 Final Year Project (FYP) http://hdl.handle.net/10356/72089 en Nanyang Technological University 72 p. application/pdf |
| spellingShingle | DRNTU::Engineering::Mechanical engineering Najmah Abdul Razak Attacking stressed Pseudomonas protegen biofilms with Wi-Fi |
| title | Attacking stressed Pseudomonas protegen biofilms with Wi-Fi |
| title_full | Attacking stressed Pseudomonas protegen biofilms with Wi-Fi |
| title_fullStr | Attacking stressed Pseudomonas protegen biofilms with Wi-Fi |
| title_full_unstemmed | Attacking stressed Pseudomonas protegen biofilms with Wi-Fi |
| title_short | Attacking stressed Pseudomonas protegen biofilms with Wi-Fi |
| title_sort | attacking stressed pseudomonas protegen biofilms with wi fi |
| topic | DRNTU::Engineering::Mechanical engineering |
| url | http://hdl.handle.net/10356/72089 |
| work_keys_str_mv | AT najmahabdulrazak attackingstressedpseudomonasprotegenbiofilmswithwifi |