Microbial source tracking and dynamics of bacterial communities and pathogens in the urban tropical environment
Research aims were (1) to examine general bacterial community composition and specific sequences characteristic of sewage associated taxa and pathogen-like sequences as a function of water quality, (2) to validate host-associated Bacteroidales genetic markers for microbial sources tracking in Singap...
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| Other Authors: | |
| Format: | Thesis |
| Language: | English |
| Published: |
2017
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| Online Access: | http://hdl.handle.net/10356/72263 |
| _version_ | 1811697403554693120 |
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| author | Nshimyimana, Jean Pierre |
| author2 | Janelle Thompson |
| author_facet | Janelle Thompson Nshimyimana, Jean Pierre |
| author_sort | Nshimyimana, Jean Pierre |
| collection | NTU |
| description | Research aims were (1) to examine general bacterial community composition and specific sequences characteristic of sewage associated taxa and pathogen-like sequences as a function of water quality, (2) to validate host-associated Bacteroidales genetic markers for microbial sources tracking in Singapore, (3) to compare the performance of digital droplet PCR and quantitative PCR in detecting and quantifying markers, and (4) to evaluate sediment-associated decay rates of the best-performing human-associated markers B. thetaiotaomicron α-1-6, mannanase (B. theta) and BacHum and those of fecal indicator bacteria in the presence and absence of grazing protozoa. Four Bacteroidales markers were validated for microbial source tracking in Singapore and Southeast Asia (Human: B. theta and BacHum; Dog: BacCan, and total Bacteroidales: BacUni). Bacteroidales viable cells
and DNA had similar decay rate constants in sediments, with two-log reduction times between five and six days, and rates were not affected by the presence of protozoa. |
| first_indexed | 2024-10-01T07:54:42Z |
| format | Thesis |
| id | ntu-10356/72263 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T07:54:42Z |
| publishDate | 2017 |
| record_format | dspace |
| spelling | ntu-10356/722632023-03-03T19:35:34Z Microbial source tracking and dynamics of bacterial communities and pathogens in the urban tropical environment Nshimyimana, Jean Pierre Janelle Thompson Stefan Wuertz School of Civil and Environmental Engineering Singapore-MIT Alliance for Research and Technology, Singapore Center for Environmental Life Sciences Engineering Singapore-MIT Alliance Programme DRNTU::Engineering::Civil engineering Research aims were (1) to examine general bacterial community composition and specific sequences characteristic of sewage associated taxa and pathogen-like sequences as a function of water quality, (2) to validate host-associated Bacteroidales genetic markers for microbial sources tracking in Singapore, (3) to compare the performance of digital droplet PCR and quantitative PCR in detecting and quantifying markers, and (4) to evaluate sediment-associated decay rates of the best-performing human-associated markers B. thetaiotaomicron α-1-6, mannanase (B. theta) and BacHum and those of fecal indicator bacteria in the presence and absence of grazing protozoa. Four Bacteroidales markers were validated for microbial source tracking in Singapore and Southeast Asia (Human: B. theta and BacHum; Dog: BacCan, and total Bacteroidales: BacUni). Bacteroidales viable cells and DNA had similar decay rate constants in sediments, with two-log reduction times between five and six days, and rates were not affected by the presence of protozoa. Doctor of Philosophy (CEE) 2017-05-31T06:25:50Z 2017-05-31T06:25:50Z 2017 Thesis Nshimyimana, J. P. (2017). Microbial source tracking and dynamics of bacterial communities and pathogens in the urban tropical environment. Doctoral thesis, Nanyang Technological University, Singapore. http://hdl.handle.net/10356/72263 10.32657/10356/72263 en 251 p. application/pdf |
| spellingShingle | DRNTU::Engineering::Civil engineering Nshimyimana, Jean Pierre Microbial source tracking and dynamics of bacterial communities and pathogens in the urban tropical environment |
| title | Microbial source tracking and dynamics of bacterial communities and pathogens in the urban tropical environment |
| title_full | Microbial source tracking and dynamics of bacterial communities and pathogens in the urban tropical environment |
| title_fullStr | Microbial source tracking and dynamics of bacterial communities and pathogens in the urban tropical environment |
| title_full_unstemmed | Microbial source tracking and dynamics of bacterial communities and pathogens in the urban tropical environment |
| title_short | Microbial source tracking and dynamics of bacterial communities and pathogens in the urban tropical environment |
| title_sort | microbial source tracking and dynamics of bacterial communities and pathogens in the urban tropical environment |
| topic | DRNTU::Engineering::Civil engineering |
| url | http://hdl.handle.net/10356/72263 |
| work_keys_str_mv | AT nshimyimanajeanpierre microbialsourcetrackinganddynamicsofbacterialcommunitiesandpathogensintheurbantropicalenvironment |