Raman spectroscopy for multiplexed protein detection in tissue bioreactors
This dissertation presents three major studies utilizing Raman spectroscopy (RS) for the detection and quantification of proteins. All studies were aimed at achieving fast, minimally invasive and cost effective protein detection to potentially complement and eventually replace current methods, such...
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| Format: | Thesis |
| Language: | English |
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2017
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| Online Access: | http://hdl.handle.net/10356/72388 |
| _version_ | 1826113581836402688 |
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| author | Perlaki, Clint Michael |
| author2 | Sierin Lim |
| author_facet | Sierin Lim Perlaki, Clint Michael |
| author_sort | Perlaki, Clint Michael |
| collection | NTU |
| description | This dissertation presents three major studies utilizing Raman spectroscopy (RS) for the detection and quantification of proteins. All studies were aimed at achieving fast, minimally invasive and cost effective protein detection to potentially complement and eventually replace current methods, such as Western blotting and ELISA, which are labor intensive and slow. Our techniques may have significant effects on biomedical research, particularly with tissue bioreactors, where there is urgent demand for new and fast protein detection technology. In this work, polarized Raman spectroscopy, Raman Optical Activity, and modulated Surface Enhanced Raman Scattering (SERS) were explored. |
| first_indexed | 2024-10-01T03:25:34Z |
| format | Thesis |
| id | ntu-10356/72388 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:25:34Z |
| publishDate | 2017 |
| record_format | dspace |
| spelling | ntu-10356/723882023-03-03T15:58:54Z Raman spectroscopy for multiplexed protein detection in tissue bioreactors Perlaki, Clint Michael Sierin Lim Quan Liu School of Chemical and Biomedical Engineering DRNTU::Science::Chemistry::Biochemistry::Spectroscopy DRNTU::Engineering::Electrical and electronic engineering::Optics, optoelectronics, photonics DRNTU::Science::Chemistry::Organic chemistry::Polymers This dissertation presents three major studies utilizing Raman spectroscopy (RS) for the detection and quantification of proteins. All studies were aimed at achieving fast, minimally invasive and cost effective protein detection to potentially complement and eventually replace current methods, such as Western blotting and ELISA, which are labor intensive and slow. Our techniques may have significant effects on biomedical research, particularly with tissue bioreactors, where there is urgent demand for new and fast protein detection technology. In this work, polarized Raman spectroscopy, Raman Optical Activity, and modulated Surface Enhanced Raman Scattering (SERS) were explored. Doctor of Philosophy (SCBE) 2017-07-03T01:57:31Z 2017-07-03T01:57:31Z 2017 Thesis Perlaki, C. M. (2017). Raman spectroscopy for multiplexed protein detection in tissue bioreactors. Doctoral thesis, Nanyang Technological University, Singapore. http://hdl.handle.net/10356/72388 10.32657/10356/72388 en 156 p. application/pdf |
| spellingShingle | DRNTU::Science::Chemistry::Biochemistry::Spectroscopy DRNTU::Engineering::Electrical and electronic engineering::Optics, optoelectronics, photonics DRNTU::Science::Chemistry::Organic chemistry::Polymers Perlaki, Clint Michael Raman spectroscopy for multiplexed protein detection in tissue bioreactors |
| title | Raman spectroscopy for multiplexed protein detection in tissue bioreactors |
| title_full | Raman spectroscopy for multiplexed protein detection in tissue bioreactors |
| title_fullStr | Raman spectroscopy for multiplexed protein detection in tissue bioreactors |
| title_full_unstemmed | Raman spectroscopy for multiplexed protein detection in tissue bioreactors |
| title_short | Raman spectroscopy for multiplexed protein detection in tissue bioreactors |
| title_sort | raman spectroscopy for multiplexed protein detection in tissue bioreactors |
| topic | DRNTU::Science::Chemistry::Biochemistry::Spectroscopy DRNTU::Engineering::Electrical and electronic engineering::Optics, optoelectronics, photonics DRNTU::Science::Chemistry::Organic chemistry::Polymers |
| url | http://hdl.handle.net/10356/72388 |
| work_keys_str_mv | AT perlakiclintmichael ramanspectroscopyformultiplexedproteindetectionintissuebioreactors |