A molecular dissection of the Rubisco activation system from rice
Ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) forms inhibited complexes with its own substrate RuBP and other sugar phosphates. The AAA+ protein Rubisco activase (Rca) counteracts this issue by removing these inhibitors and thus maintaining Rubisco in its functional state. Inactivation o...
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| Other Authors: | |
| Format: | Thesis |
| Language: | English |
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2017
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| Online Access: | http://hdl.handle.net/10356/72402 |
| _version_ | 1826128800939769856 |
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| author | Shivhare, Devendra |
| author2 | Oliver Martin Mueller-Cajar |
| author_facet | Oliver Martin Mueller-Cajar Shivhare, Devendra |
| author_sort | Shivhare, Devendra |
| collection | NTU |
| description | Ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) forms inhibited complexes with its own substrate RuBP and other sugar phosphates. The AAA+ protein Rubisco activase (Rca) counteracts this issue by removing these inhibitors and thus maintaining Rubisco in its functional state. Inactivation of Rca at moderately high temperatures is associated with the loss of the activation state of Rubisco. Here we present a detailed characterization of the Rubisco activase from rice (Oryza sativa). We then compare its properties to a highly functional and thermostable Rca system from the desert plant, Agave tequilana. An extensive mutational study identified a number of residues that are likely involved in the Rubisco-Rca interaction. Finally we present a modified model for Rubisco activation in plants. Our results indicate that bioprospecting and careful biochemical characterization of Rca homologues will lead to both mechanistic insights and identification of candidate proteins for engineering enhanced thermotolerance in rice. |
| first_indexed | 2024-10-01T07:30:50Z |
| format | Thesis |
| id | ntu-10356/72402 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T07:30:50Z |
| publishDate | 2017 |
| record_format | dspace |
| spelling | ntu-10356/724022023-02-28T18:46:52Z A molecular dissection of the Rubisco activation system from rice Shivhare, Devendra Oliver Martin Mueller-Cajar School of Biological Sciences DRNTU::Science::Biological sciences::Biochemistry Ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) forms inhibited complexes with its own substrate RuBP and other sugar phosphates. The AAA+ protein Rubisco activase (Rca) counteracts this issue by removing these inhibitors and thus maintaining Rubisco in its functional state. Inactivation of Rca at moderately high temperatures is associated with the loss of the activation state of Rubisco. Here we present a detailed characterization of the Rubisco activase from rice (Oryza sativa). We then compare its properties to a highly functional and thermostable Rca system from the desert plant, Agave tequilana. An extensive mutational study identified a number of residues that are likely involved in the Rubisco-Rca interaction. Finally we present a modified model for Rubisco activation in plants. Our results indicate that bioprospecting and careful biochemical characterization of Rca homologues will lead to both mechanistic insights and identification of candidate proteins for engineering enhanced thermotolerance in rice. Doctor of Philosophy (SBS) 2017-07-10T01:34:55Z 2017-07-10T01:34:55Z 2017 Thesis Shivhare, D. (2017). A molecular dissection of the Rubisco activation system from rice. Doctoral thesis, Nanyang Technological University, Singapore. http://hdl.handle.net/10356/72402 10.32657/10356/72402 en 190 p. application/pdf |
| spellingShingle | DRNTU::Science::Biological sciences::Biochemistry Shivhare, Devendra A molecular dissection of the Rubisco activation system from rice |
| title | A molecular dissection of the Rubisco activation system from rice |
| title_full | A molecular dissection of the Rubisco activation system from rice |
| title_fullStr | A molecular dissection of the Rubisco activation system from rice |
| title_full_unstemmed | A molecular dissection of the Rubisco activation system from rice |
| title_short | A molecular dissection of the Rubisco activation system from rice |
| title_sort | molecular dissection of the rubisco activation system from rice |
| topic | DRNTU::Science::Biological sciences::Biochemistry |
| url | http://hdl.handle.net/10356/72402 |
| work_keys_str_mv | AT shivharedevendra amoleculardissectionoftherubiscoactivationsystemfromrice AT shivharedevendra moleculardissectionoftherubiscoactivationsystemfromrice |