The role of Importin-7 (IPO7) during activity-dependent nucleocytoplasmic shuttling of transcription modulators in hippocampal neurons
Importin-7, a member of the importin-beta superfamily of nuclear adaptor proteins, is thought to shuttle activated MAPK into the nucleus to induce transcription-dependent encoding of long-term memories in Drosophila. However, little is known about the function of importin-7, its cellular distributio...
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| Format: | Final Year Project (FYP) |
| Language: | English |
| Published: |
2017
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| Online Access: | http://hdl.handle.net/10356/72466 |
| _version_ | 1826109570592800768 |
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| author | Chin, Pei Wern |
| author2 | Ch'ng Toh Hean |
| author_facet | Ch'ng Toh Hean Chin, Pei Wern |
| author_sort | Chin, Pei Wern |
| collection | NTU |
| description | Importin-7, a member of the importin-beta superfamily of nuclear adaptor proteins, is thought to shuttle activated MAPK into the nucleus to induce transcription-dependent encoding of long-term memories in Drosophila. However, little is known about the function of importin-7, its cellular distribution, mechanisms of action and the additional cargo proteins it binds to. This project aims to characterize importin-7 in rodent hippocampal neurons with the ultimate goal of deciphering its function during learning and memory. Our initial findings indicate that importin-7 is localized at the cytosol, dendrites and spines in mature hippocampal neurons. We found that cytosolic importin- 7 undergoes activity-dependent translocation into the nucleus upon chemical long-term potentiation in acute whole brain slices. To identify cargo proteins that bind to importin-7 in the brain, we performed co-immunoprecipitation studies for importin-7 and analyzed affinity-purified proteins via mass spectrometry, but results are currently pending. Overall, our initial characterization studies hint that importin-7 could be involved in the activity-dependent transport of synaptic proteins into the nucleus during stimuli associated with transcription-dependent long-term plasticity. |
| first_indexed | 2024-10-01T02:20:24Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/72466 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T02:20:24Z |
| publishDate | 2017 |
| record_format | dspace |
| spelling | ntu-10356/724662023-02-28T17:59:55Z The role of Importin-7 (IPO7) during activity-dependent nucleocytoplasmic shuttling of transcription modulators in hippocampal neurons Chin, Pei Wern Ch'ng Toh Hean School of Biological Sciences DRNTU::Science::Biological sciences::Molecular biology Importin-7, a member of the importin-beta superfamily of nuclear adaptor proteins, is thought to shuttle activated MAPK into the nucleus to induce transcription-dependent encoding of long-term memories in Drosophila. However, little is known about the function of importin-7, its cellular distribution, mechanisms of action and the additional cargo proteins it binds to. This project aims to characterize importin-7 in rodent hippocampal neurons with the ultimate goal of deciphering its function during learning and memory. Our initial findings indicate that importin-7 is localized at the cytosol, dendrites and spines in mature hippocampal neurons. We found that cytosolic importin- 7 undergoes activity-dependent translocation into the nucleus upon chemical long-term potentiation in acute whole brain slices. To identify cargo proteins that bind to importin-7 in the brain, we performed co-immunoprecipitation studies for importin-7 and analyzed affinity-purified proteins via mass spectrometry, but results are currently pending. Overall, our initial characterization studies hint that importin-7 could be involved in the activity-dependent transport of synaptic proteins into the nucleus during stimuli associated with transcription-dependent long-term plasticity. Bachelor of Science in Biological Sciences 2017-07-31T01:06:13Z 2017-07-31T01:06:13Z 2017 Final Year Project (FYP) http://hdl.handle.net/10356/72466 en Nanyang Technological University 28 p. application/pdf |
| spellingShingle | DRNTU::Science::Biological sciences::Molecular biology Chin, Pei Wern The role of Importin-7 (IPO7) during activity-dependent nucleocytoplasmic shuttling of transcription modulators in hippocampal neurons |
| title | The role of Importin-7 (IPO7) during activity-dependent nucleocytoplasmic shuttling of transcription modulators in hippocampal neurons |
| title_full | The role of Importin-7 (IPO7) during activity-dependent nucleocytoplasmic shuttling of transcription modulators in hippocampal neurons |
| title_fullStr | The role of Importin-7 (IPO7) during activity-dependent nucleocytoplasmic shuttling of transcription modulators in hippocampal neurons |
| title_full_unstemmed | The role of Importin-7 (IPO7) during activity-dependent nucleocytoplasmic shuttling of transcription modulators in hippocampal neurons |
| title_short | The role of Importin-7 (IPO7) during activity-dependent nucleocytoplasmic shuttling of transcription modulators in hippocampal neurons |
| title_sort | role of importin 7 ipo7 during activity dependent nucleocytoplasmic shuttling of transcription modulators in hippocampal neurons |
| topic | DRNTU::Science::Biological sciences::Molecular biology |
| url | http://hdl.handle.net/10356/72466 |
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