Novel imaging approaches to track the movement of proteins and small molecules within bacterial cells and biofilms
Bacterial cells often exist in the form of sessile aggregates known as biofilms. Such biofilms are capable of producing adhesive Extracellular Polymeric Substances (EPS) or matrix. The biofilm matrix is often composed of biopolymers such as polysaccharide, proteins, and extracellular DNA (eDNA). Add...
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| Format: | Thesis |
| Language: | English |
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2017
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| Online Access: | http://hdl.handle.net/10356/72495 |
| _version_ | 1811694239307792384 |
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| author | Kumaravel Kandaswamy |
| author2 | Kimberly Kline |
| author_facet | Kimberly Kline Kumaravel Kandaswamy |
| author_sort | Kumaravel Kandaswamy |
| collection | NTU |
| description | Bacterial cells often exist in the form of sessile aggregates known as biofilms. Such biofilms are capable of producing adhesive Extracellular Polymeric Substances (EPS) or matrix. The biofilm matrix is often composed of biopolymers such as polysaccharide, proteins, and extracellular DNA (eDNA). Additionally, biofilms also contains phage particles that play a protective role during early stages of biofilm cycle. The matrix of the biofilm is also associated with establishment of chemical gradients, which contributes significantly to the physiological heterogeneity of the biofilm. The aim of this research was to demonstrate the use of fluorescence microscopy techniques to better understand and quantify the spatial organization of proteins and polysaccharides in single cell (Enterococcus faecalis) and biofilm (Pseudomonas aeruginosa) model systems, respectively. In doing so, high-throughput immunofluorescence assay was used to quantify the phenotypic variation in protein localization patterns in hundreds of E. faecalis cells. Furthermore, using Fluorescence Correlation Spectroscopy (FCS) the absolute diffusion coefficients of dextran molecules and phage particles entering P. aeruginosa biofilms was estimated. In essence, this research will provide technical insights on applicability of imaging tools to quantify the process occurring in single cell and biofilm model systems. |
| first_indexed | 2024-10-01T07:04:25Z |
| format | Thesis |
| id | ntu-10356/72495 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T07:04:25Z |
| publishDate | 2017 |
| record_format | dspace |
| spelling | ntu-10356/724952021-03-20T13:58:41Z Novel imaging approaches to track the movement of proteins and small molecules within bacterial cells and biofilms Kumaravel Kandaswamy Kimberly Kline Cohen Yehuda Interdisciplinary Graduate School (IGS) Environmental Engineering Research Centre DRNTU::Engineering::Chemical engineering Bacterial cells often exist in the form of sessile aggregates known as biofilms. Such biofilms are capable of producing adhesive Extracellular Polymeric Substances (EPS) or matrix. The biofilm matrix is often composed of biopolymers such as polysaccharide, proteins, and extracellular DNA (eDNA). Additionally, biofilms also contains phage particles that play a protective role during early stages of biofilm cycle. The matrix of the biofilm is also associated with establishment of chemical gradients, which contributes significantly to the physiological heterogeneity of the biofilm. The aim of this research was to demonstrate the use of fluorescence microscopy techniques to better understand and quantify the spatial organization of proteins and polysaccharides in single cell (Enterococcus faecalis) and biofilm (Pseudomonas aeruginosa) model systems, respectively. In doing so, high-throughput immunofluorescence assay was used to quantify the phenotypic variation in protein localization patterns in hundreds of E. faecalis cells. Furthermore, using Fluorescence Correlation Spectroscopy (FCS) the absolute diffusion coefficients of dextran molecules and phage particles entering P. aeruginosa biofilms was estimated. In essence, this research will provide technical insights on applicability of imaging tools to quantify the process occurring in single cell and biofilm model systems. Doctor of Philosophy (IGS) 2017-08-16T07:39:13Z 2017-08-16T07:39:13Z 2017 Thesis Kumaravel Kandaswamy. (2017). Novel imaging approaches to track the movement of proteins and small molecules within bacterial cells and biofilms. Doctoral thesis, Nanyang Technological University, Singapore. http://hdl.handle.net/10356/72495 10.32657/10356/72495 en 136 p. application/pdf |
| spellingShingle | DRNTU::Engineering::Chemical engineering Kumaravel Kandaswamy Novel imaging approaches to track the movement of proteins and small molecules within bacterial cells and biofilms |
| title | Novel imaging approaches to track the movement of proteins and small molecules within bacterial cells and biofilms |
| title_full | Novel imaging approaches to track the movement of proteins and small molecules within bacterial cells and biofilms |
| title_fullStr | Novel imaging approaches to track the movement of proteins and small molecules within bacterial cells and biofilms |
| title_full_unstemmed | Novel imaging approaches to track the movement of proteins and small molecules within bacterial cells and biofilms |
| title_short | Novel imaging approaches to track the movement of proteins and small molecules within bacterial cells and biofilms |
| title_sort | novel imaging approaches to track the movement of proteins and small molecules within bacterial cells and biofilms |
| topic | DRNTU::Engineering::Chemical engineering |
| url | http://hdl.handle.net/10356/72495 |
| work_keys_str_mv | AT kumaravelkandaswamy novelimagingapproachestotrackthemovementofproteinsandsmallmoleculeswithinbacterialcellsandbiofilms |