Visual feedback for a semi-automatic micropipette aspiration of single biological cells
This project aims to provide visual feedback to a system to mechanically characterize single white blood cells by micropipette aspiration. In this project, image processing algorithms were developed to detect individual cells and the micropipette, to provide feedback to a system to control linear...
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| Other Authors: | |
| Format: | Thesis |
| Language: | English |
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2017
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| Online Access: | http://hdl.handle.net/10356/72567 |
| _version_ | 1826121453111607296 |
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| author | Wang, Zili |
| author2 | Justin Dauwels |
| author_facet | Justin Dauwels Wang, Zili |
| author_sort | Wang, Zili |
| collection | NTU |
| description | This project aims to provide visual feedback to a system to mechanically characterize
single white blood cells by micropipette aspiration. In this project, image processing
algorithms were developed to detect individual cells and the micropipette, to provide
feedback to a system to control linear actuators and position a micropipette to approach
a cell, form a seal and partially aspirate the cell. Circular Hough Transform was used
to detect cells, Normalized Cross-Correlation was used to do template-match and
detect the micropipette and Kalman Fiter was used to track the movements. Under a
bright field microscope, monocytes (a type of white blood cell) are irregularly shaped,
furthermore, these cells may cluster together. Therefore, the circular Hough transform
failed to accurately detect the cells. An improved algorithm which can find clusters
and segment cells was developed by using Mathematical Morphology and K-means
clustering. At the end of this project, the visual feedback algorithms perform well, and
can potentially be used as a fundament to support the vision-based control of
micropipette aspiration as well as the study of cell structures and relevant disease
progressions. |
| first_indexed | 2024-10-01T05:32:53Z |
| format | Thesis |
| id | ntu-10356/72567 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T05:32:53Z |
| publishDate | 2017 |
| record_format | dspace |
| spelling | ntu-10356/725672023-07-04T15:53:31Z Visual feedback for a semi-automatic micropipette aspiration of single biological cells Wang, Zili Justin Dauwels School of Electrical and Electronic Engineering DRNTU::Engineering::Electrical and electronic engineering This project aims to provide visual feedback to a system to mechanically characterize single white blood cells by micropipette aspiration. In this project, image processing algorithms were developed to detect individual cells and the micropipette, to provide feedback to a system to control linear actuators and position a micropipette to approach a cell, form a seal and partially aspirate the cell. Circular Hough Transform was used to detect cells, Normalized Cross-Correlation was used to do template-match and detect the micropipette and Kalman Fiter was used to track the movements. Under a bright field microscope, monocytes (a type of white blood cell) are irregularly shaped, furthermore, these cells may cluster together. Therefore, the circular Hough transform failed to accurately detect the cells. An improved algorithm which can find clusters and segment cells was developed by using Mathematical Morphology and K-means clustering. At the end of this project, the visual feedback algorithms perform well, and can potentially be used as a fundament to support the vision-based control of micropipette aspiration as well as the study of cell structures and relevant disease progressions. Master of Science (Computer Control and Automation) 2017-08-28T12:43:12Z 2017-08-28T12:43:12Z 2017 Thesis http://hdl.handle.net/10356/72567 en 52 p. application/pdf |
| spellingShingle | DRNTU::Engineering::Electrical and electronic engineering Wang, Zili Visual feedback for a semi-automatic micropipette aspiration of single biological cells |
| title | Visual feedback for a semi-automatic micropipette aspiration of single biological cells |
| title_full | Visual feedback for a semi-automatic micropipette aspiration of single biological cells |
| title_fullStr | Visual feedback for a semi-automatic micropipette aspiration of single biological cells |
| title_full_unstemmed | Visual feedback for a semi-automatic micropipette aspiration of single biological cells |
| title_short | Visual feedback for a semi-automatic micropipette aspiration of single biological cells |
| title_sort | visual feedback for a semi automatic micropipette aspiration of single biological cells |
| topic | DRNTU::Engineering::Electrical and electronic engineering |
| url | http://hdl.handle.net/10356/72567 |
| work_keys_str_mv | AT wangzili visualfeedbackforasemiautomaticmicropipetteaspirationofsinglebiologicalcells |