Mimicking nanofibrous hybrid substitute for bone tissue engineering
Bone grafts are increasingly used for correcting bone fractures defects. Massive implants usually result in slow healing. The aim of this study is to fabricate nanofibrous scaffolds using Polycaprolactone/silk fibroin/Zinc coated silver nanoparticles (PCL/SF/Ag(Zn)) for bone tissue engineering. Hu...
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| Other Authors: | |
| Format: | Final Year Project (FYP) |
| Language: | English |
| Published: |
2017
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| Online Access: | http://hdl.handle.net/10356/72624 |
| _version_ | 1811677859807232000 |
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| author | Wong, Gabriel Liang Jie |
| author2 | Dinesh Srinivasan |
| author_facet | Dinesh Srinivasan Wong, Gabriel Liang Jie |
| author_sort | Wong, Gabriel Liang Jie |
| collection | NTU |
| description | Bone grafts are increasingly used for correcting bone fractures defects. Massive implants usually result
in slow healing. The aim of this study is to fabricate nanofibrous scaffolds using Polycaprolactone/silk
fibroin/Zinc coated silver nanoparticles (PCL/SF/Ag(Zn)) for bone tissue engineering. Human
Mesenchymal Stem cells (hMSCs) were seeded onto the composite scaffolds and allowed to grow for 5,
10 and 15 days. They were then tested for cell proliferation via MTS assay, mineralization qualitatively
via Alizarin red-S dye and live cell morphology via CMFDA dye exclusion methods. The results were that
we found cell proliferation increased with addition of Silk fibroin and even more with addition of Zinc
coated silver nanoparticles. Cell morphology was normal and hence suitable with PCL, but morphology
was more of a more differentiated form with zinc coated silver nanoparticles and silk fibroin samples.
Also, Mineralization was seen to be increased in the samples with silk fibroin, zinc coated silver
nanoparticles. Hence, this suggested that fabricated PCL/SF/Ag (Zn) nanofibrous scaffolds have potential
for use in bone tissue engineering for differentiation of MSCs. |
| first_indexed | 2024-10-01T02:44:04Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/72624 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T02:44:04Z |
| publishDate | 2017 |
| record_format | dspace |
| spelling | ntu-10356/726242020-11-01T05:32:42Z Mimicking nanofibrous hybrid substitute for bone tissue engineering Wong, Gabriel Liang Jie Dinesh Srinivasan Lee Kong Chian School of Medicine (LKCMedicine) DRNTU::Science::Medicine Bone grafts are increasingly used for correcting bone fractures defects. Massive implants usually result in slow healing. The aim of this study is to fabricate nanofibrous scaffolds using Polycaprolactone/silk fibroin/Zinc coated silver nanoparticles (PCL/SF/Ag(Zn)) for bone tissue engineering. Human Mesenchymal Stem cells (hMSCs) were seeded onto the composite scaffolds and allowed to grow for 5, 10 and 15 days. They were then tested for cell proliferation via MTS assay, mineralization qualitatively via Alizarin red-S dye and live cell morphology via CMFDA dye exclusion methods. The results were that we found cell proliferation increased with addition of Silk fibroin and even more with addition of Zinc coated silver nanoparticles. Cell morphology was normal and hence suitable with PCL, but morphology was more of a more differentiated form with zinc coated silver nanoparticles and silk fibroin samples. Also, Mineralization was seen to be increased in the samples with silk fibroin, zinc coated silver nanoparticles. Hence, this suggested that fabricated PCL/SF/Ag (Zn) nanofibrous scaffolds have potential for use in bone tissue engineering for differentiation of MSCs. Bachelor of Medicine and Bachelor of Surgery 2017-08-30T08:33:49Z 2017-08-30T08:33:49Z 2017 Final Year Project (FYP) http://hdl.handle.net/10356/72624 en 9 p. application/pdf |
| spellingShingle | DRNTU::Science::Medicine Wong, Gabriel Liang Jie Mimicking nanofibrous hybrid substitute for bone tissue engineering |
| title | Mimicking nanofibrous hybrid substitute for bone tissue engineering |
| title_full | Mimicking nanofibrous hybrid substitute for bone tissue engineering |
| title_fullStr | Mimicking nanofibrous hybrid substitute for bone tissue engineering |
| title_full_unstemmed | Mimicking nanofibrous hybrid substitute for bone tissue engineering |
| title_short | Mimicking nanofibrous hybrid substitute for bone tissue engineering |
| title_sort | mimicking nanofibrous hybrid substitute for bone tissue engineering |
| topic | DRNTU::Science::Medicine |
| url | http://hdl.handle.net/10356/72624 |
| work_keys_str_mv | AT wonggabrielliangjie mimickingnanofibroushybridsubstituteforbonetissueengineering |