Extracellular unit recording study in characterizing activity of optogenetically identified parvalbumin expressing neurons in dentate gyrus of freely moving mice
Information processing in the DG relies on the interactions between activity of excitatory and inhibitory neurons. Inhibitory neurons are not a homogeneous population. It is interesting to know how each subtype of inhibitory neurons control the activity of excitatory neurons. Traditional extracellul...
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| Other Authors: | |
| Format: | Thesis |
| Language: | English |
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2017
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| Online Access: | http://hdl.handle.net/10356/72720 |
| _version_ | 1826117187486613504 |
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| author | Lit, Kwok Keung |
| author2 | Ayumu Tashiro |
| author_facet | Ayumu Tashiro Lit, Kwok Keung |
| author_sort | Lit, Kwok Keung |
| collection | NTU |
| description | Information processing in the DG relies on the interactions between activity of excitatory and inhibitory neurons. Inhibitory neurons are not a homogeneous population. It is interesting to know how each subtype of inhibitory neurons control the activity of excitatory neurons. Traditional extracellular unit recording lacks the ability to identify neuronal types, despite its advantage in simultaneously detecting activity of multiple neurons. Here we used a transgenic mouse line that express Channelrhopsin-2 (ChR2), a light-activated ion channel, in a subtype of inhibitory neurons that also express parvalbumin (PV). By identifying their firing response to optical stimulation and firing during exploration, we were able to characterize the firing pattern of these neurons during spatial exploration of the animal. The result contributes to understanding how processing of spatial information is achieved in the dentate gyrus. |
| first_indexed | 2024-10-01T04:23:31Z |
| format | Thesis |
| id | ntu-10356/72720 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:23:31Z |
| publishDate | 2017 |
| record_format | dspace |
| spelling | ntu-10356/727202023-02-28T18:37:29Z Extracellular unit recording study in characterizing activity of optogenetically identified parvalbumin expressing neurons in dentate gyrus of freely moving mice Lit, Kwok Keung Ayumu Tashiro School of Biological Sciences DRNTU::Science::Biological sciences::Human anatomy and physiology::Neurobiology Information processing in the DG relies on the interactions between activity of excitatory and inhibitory neurons. Inhibitory neurons are not a homogeneous population. It is interesting to know how each subtype of inhibitory neurons control the activity of excitatory neurons. Traditional extracellular unit recording lacks the ability to identify neuronal types, despite its advantage in simultaneously detecting activity of multiple neurons. Here we used a transgenic mouse line that express Channelrhopsin-2 (ChR2), a light-activated ion channel, in a subtype of inhibitory neurons that also express parvalbumin (PV). By identifying their firing response to optical stimulation and firing during exploration, we were able to characterize the firing pattern of these neurons during spatial exploration of the animal. The result contributes to understanding how processing of spatial information is achieved in the dentate gyrus. Master of Science 2017-10-26T09:10:38Z 2017-10-26T09:10:38Z 2017 Thesis Lit, K. K. (2017). Extracellular unit recording study in characterizing activity of optogenetically identified parvalbumin expressing neurons in dentate gyrus of freely moving mice. Master's thesis, Nanyang Technological University, Singapore. http://hdl.handle.net/10356/72720 10.32657/10356/72720 en 104 p. application/pdf |
| spellingShingle | DRNTU::Science::Biological sciences::Human anatomy and physiology::Neurobiology Lit, Kwok Keung Extracellular unit recording study in characterizing activity of optogenetically identified parvalbumin expressing neurons in dentate gyrus of freely moving mice |
| title | Extracellular unit recording study in characterizing activity of optogenetically identified parvalbumin expressing neurons in dentate gyrus of freely moving mice |
| title_full | Extracellular unit recording study in characterizing activity of optogenetically identified parvalbumin expressing neurons in dentate gyrus of freely moving mice |
| title_fullStr | Extracellular unit recording study in characterizing activity of optogenetically identified parvalbumin expressing neurons in dentate gyrus of freely moving mice |
| title_full_unstemmed | Extracellular unit recording study in characterizing activity of optogenetically identified parvalbumin expressing neurons in dentate gyrus of freely moving mice |
| title_short | Extracellular unit recording study in characterizing activity of optogenetically identified parvalbumin expressing neurons in dentate gyrus of freely moving mice |
| title_sort | extracellular unit recording study in characterizing activity of optogenetically identified parvalbumin expressing neurons in dentate gyrus of freely moving mice |
| topic | DRNTU::Science::Biological sciences::Human anatomy and physiology::Neurobiology |
| url | http://hdl.handle.net/10356/72720 |
| work_keys_str_mv | AT litkwokkeung extracellularunitrecordingstudyincharacterizingactivityofoptogeneticallyidentifiedparvalbuminexpressingneuronsindentategyrusoffreelymovingmice |