Development of antifungal biopharmaceutical dectin1-FC fusion
Invasive fungal infections are a neglected category of disease that has a high mortality and implicated in immunocompromised patients. Dectin-1 (CLEC7A) is a type II transmembrane C-type lectin pattern recognition receptor expressed on innate immune cells such as macrophages, monocytes and neutrophi...
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| Format: | Final Year Project (FYP) |
| Language: | English |
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2018
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| Online Access: | http://hdl.handle.net/10356/75181 |
| _version_ | 1826115852888440832 |
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| author | Goh, Melody |
| author2 | Chen Wei Ning, William |
| author_facet | Chen Wei Ning, William Goh, Melody |
| author_sort | Goh, Melody |
| collection | NTU |
| description | Invasive fungal infections are a neglected category of disease that has a high mortality and implicated in immunocompromised patients. Dectin-1 (CLEC7A) is a type II transmembrane C-type lectin pattern recognition receptor expressed on innate immune cells such as macrophages, monocytes and neutrophils. These cells identify β-glucans via its single C-type lectin-like domain on its extracellular region. It activates by detecting and binding to cell walls of different types of fungal; triggering intracellular responses like phagocytosis and cytokine production. The absence of β-glucans in human cells presents the opportunity to exploit Dectin-1 as therapeutic vehicle to enhance immune response during a fungal infection. This project aims to develop a soluble Dectin-1 immunoglobulin Fc fragment fusion protein as a potential biopharmaceutical against fungal infections and investigate its ability to enhance phagocytic activity in immune cells. His-trap purification column will also be optimized in this project to obtain optimum purification conditions for this protein. |
| first_indexed | 2024-10-01T04:01:54Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/75181 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:01:54Z |
| publishDate | 2018 |
| record_format | dspace |
| spelling | ntu-10356/751812023-03-03T15:34:24Z Development of antifungal biopharmaceutical dectin1-FC fusion Goh, Melody Chen Wei Ning, William School of Chemical and Biomedical Engineering A*STAR Bioprocessing Technology Institute Ng Say Kong DRNTU::Engineering::Bioengineering Invasive fungal infections are a neglected category of disease that has a high mortality and implicated in immunocompromised patients. Dectin-1 (CLEC7A) is a type II transmembrane C-type lectin pattern recognition receptor expressed on innate immune cells such as macrophages, monocytes and neutrophils. These cells identify β-glucans via its single C-type lectin-like domain on its extracellular region. It activates by detecting and binding to cell walls of different types of fungal; triggering intracellular responses like phagocytosis and cytokine production. The absence of β-glucans in human cells presents the opportunity to exploit Dectin-1 as therapeutic vehicle to enhance immune response during a fungal infection. This project aims to develop a soluble Dectin-1 immunoglobulin Fc fragment fusion protein as a potential biopharmaceutical against fungal infections and investigate its ability to enhance phagocytic activity in immune cells. His-trap purification column will also be optimized in this project to obtain optimum purification conditions for this protein. Bachelor of Engineering (Chemical and Biomolecular Engineering) 2018-05-30T01:14:18Z 2018-05-30T01:14:18Z 2018 Final Year Project (FYP) http://hdl.handle.net/10356/75181 en Nanyang Technological University 42 p. application/pdf |
| spellingShingle | DRNTU::Engineering::Bioengineering Goh, Melody Development of antifungal biopharmaceutical dectin1-FC fusion |
| title | Development of antifungal biopharmaceutical dectin1-FC fusion |
| title_full | Development of antifungal biopharmaceutical dectin1-FC fusion |
| title_fullStr | Development of antifungal biopharmaceutical dectin1-FC fusion |
| title_full_unstemmed | Development of antifungal biopharmaceutical dectin1-FC fusion |
| title_short | Development of antifungal biopharmaceutical dectin1-FC fusion |
| title_sort | development of antifungal biopharmaceutical dectin1 fc fusion |
| topic | DRNTU::Engineering::Bioengineering |
| url | http://hdl.handle.net/10356/75181 |
| work_keys_str_mv | AT gohmelody developmentofantifungalbiopharmaceuticaldectin1fcfusion |