Efficiency enhancement of Raman spectroscopy at long working distance by parabolic reflector
Raman spectroscopy is well suited for readily revealing information about bio-samples. As such, this technique has been applied to a wide range of areas, particularly in bio-medical diagnostics. Raman scattering in bio-samples typically has a low signal level due to the nature of inelastic scatterin...
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| Other Authors: | |
| Format: | Thesis |
| Language: | English |
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2018
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| Online Access: | http://hdl.handle.net/10356/75263 |
| _version_ | 1826128678472384512 |
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| author | Su, Joshua Weiming |
| author2 | Liu Quan |
| author_facet | Liu Quan Su, Joshua Weiming |
| author_sort | Su, Joshua Weiming |
| collection | NTU |
| description | Raman spectroscopy is well suited for readily revealing information about bio-samples. As such, this technique has been applied to a wide range of areas, particularly in bio-medical diagnostics. Raman scattering in bio-samples typically has a low signal level due to the nature of inelastic scattering of photons. To achieve a high signal level, usually a high numerical aperture objective is employed. One drawback with these objectives is that their working distance is very short. However, in many cases of clinical diagnostics, a long working distance is preferable. We propose a practical solution to this problem by enhancing the Raman signal using a parabolic reflector. The high signal level is achieved through the large light collection solid angle of the parabolic reflector while the long working distance is ensured by the novel design of our microscope. The enhancement capability of the microscope was demonstrated on four types of samples. Among these samples, we find that this microscope design is most suitable for turbid samples. |
| first_indexed | 2024-10-01T07:28:52Z |
| format | Thesis |
| id | ntu-10356/75263 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T07:28:52Z |
| publishDate | 2018 |
| record_format | dspace |
| spelling | ntu-10356/752632023-03-03T16:06:25Z Efficiency enhancement of Raman spectroscopy at long working distance by parabolic reflector Su, Joshua Weiming Liu Quan School of Chemical and Biomedical Engineering DRNTU::Engineering::Bioengineering Raman spectroscopy is well suited for readily revealing information about bio-samples. As such, this technique has been applied to a wide range of areas, particularly in bio-medical diagnostics. Raman scattering in bio-samples typically has a low signal level due to the nature of inelastic scattering of photons. To achieve a high signal level, usually a high numerical aperture objective is employed. One drawback with these objectives is that their working distance is very short. However, in many cases of clinical diagnostics, a long working distance is preferable. We propose a practical solution to this problem by enhancing the Raman signal using a parabolic reflector. The high signal level is achieved through the large light collection solid angle of the parabolic reflector while the long working distance is ensured by the novel design of our microscope. The enhancement capability of the microscope was demonstrated on four types of samples. Among these samples, we find that this microscope design is most suitable for turbid samples. Master of Science (Biomedical Engineering) 2018-05-30T06:50:38Z 2018-05-30T06:50:38Z 2018 Thesis http://hdl.handle.net/10356/75263 en 37 p. application/pdf |
| spellingShingle | DRNTU::Engineering::Bioengineering Su, Joshua Weiming Efficiency enhancement of Raman spectroscopy at long working distance by parabolic reflector |
| title | Efficiency enhancement of Raman spectroscopy at long working distance by parabolic reflector |
| title_full | Efficiency enhancement of Raman spectroscopy at long working distance by parabolic reflector |
| title_fullStr | Efficiency enhancement of Raman spectroscopy at long working distance by parabolic reflector |
| title_full_unstemmed | Efficiency enhancement of Raman spectroscopy at long working distance by parabolic reflector |
| title_short | Efficiency enhancement of Raman spectroscopy at long working distance by parabolic reflector |
| title_sort | efficiency enhancement of raman spectroscopy at long working distance by parabolic reflector |
| topic | DRNTU::Engineering::Bioengineering |
| url | http://hdl.handle.net/10356/75263 |
| work_keys_str_mv | AT sujoshuaweiming efficiencyenhancementoframanspectroscopyatlongworkingdistancebyparabolicreflector |