Designer substrates to engineer stem cells secretome production
Angiogenesis holds an important role in wound healing by constructing new blood vessels from the preexisting vessels through the invasion of injury clot. This process is largely regulated by signals from both the surrounding extracellular matrix (ECM) environment and serum. In hopes to have deeper u...
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| Format: | Final Year Project (FYP) |
| Language: | English |
| Published: |
2019
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| Online Access: | http://hdl.handle.net/10356/77270 |
| _version_ | 1811695406883536896 |
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| author | Tay, Sharon Wee Sin |
| author2 | Dalton Tay Chor Yong |
| author_facet | Dalton Tay Chor Yong Tay, Sharon Wee Sin |
| author_sort | Tay, Sharon Wee Sin |
| collection | NTU |
| description | Angiogenesis holds an important role in wound healing by constructing new blood vessels from the preexisting vessels through the invasion of injury clot. This process is largely regulated by signals from both the surrounding extracellular matrix (ECM) environment and serum. In hopes to have deeper understanding towards stem cells activity, hydrogels is often used in studies to biomimick ECM. In this study, fibronectin-conjugated polyacrylamide (PAA) hydrogel with adjustable stiffness is exploited to regulate the intracellular oxidative state of adipose derived stem cells (ADSCs). Our results have shown hydrogels of varied kPa stiffness increase the reactive oxidative species(ROS) levels in ADSCs as compared to ADSCs cultured on fibronectin coated glass slip control in an almost non-cytoxic way. The concoction
of secreted proteins by ADSCs can be modulated by intracellular ROS levels via the adjustable hydrogel substrate stiffness. The secretome or conditioned medium(CM) retrieved from the ADSCs cultured on varied stiffness substrate, were thereafter cultured with human umbilical vein endothelial cells (HUVECs) and results have shown that the CM from ADSCs on the softest substrate promotes angiogenesis. Findings from this study shows that soft materials can be a novel approach to induce oxidative stress in MSCs to stimulate them to secrete pro-healing factors. |
| first_indexed | 2024-10-01T07:22:58Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/77270 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T07:22:58Z |
| publishDate | 2019 |
| record_format | dspace |
| spelling | ntu-10356/772702023-03-04T15:37:35Z Designer substrates to engineer stem cells secretome production Tay, Sharon Wee Sin Dalton Tay Chor Yong School of Materials Science and Engineering DRNTU::Engineering::Materials Angiogenesis holds an important role in wound healing by constructing new blood vessels from the preexisting vessels through the invasion of injury clot. This process is largely regulated by signals from both the surrounding extracellular matrix (ECM) environment and serum. In hopes to have deeper understanding towards stem cells activity, hydrogels is often used in studies to biomimick ECM. In this study, fibronectin-conjugated polyacrylamide (PAA) hydrogel with adjustable stiffness is exploited to regulate the intracellular oxidative state of adipose derived stem cells (ADSCs). Our results have shown hydrogels of varied kPa stiffness increase the reactive oxidative species(ROS) levels in ADSCs as compared to ADSCs cultured on fibronectin coated glass slip control in an almost non-cytoxic way. The concoction of secreted proteins by ADSCs can be modulated by intracellular ROS levels via the adjustable hydrogel substrate stiffness. The secretome or conditioned medium(CM) retrieved from the ADSCs cultured on varied stiffness substrate, were thereafter cultured with human umbilical vein endothelial cells (HUVECs) and results have shown that the CM from ADSCs on the softest substrate promotes angiogenesis. Findings from this study shows that soft materials can be a novel approach to induce oxidative stress in MSCs to stimulate them to secrete pro-healing factors. Bachelor of Engineering (Materials Engineering) 2019-05-23T09:14:05Z 2019-05-23T09:14:05Z 2019 Final Year Project (FYP) http://hdl.handle.net/10356/77270 en Nanyang Technological University 50 p. application/pdf |
| spellingShingle | DRNTU::Engineering::Materials Tay, Sharon Wee Sin Designer substrates to engineer stem cells secretome production |
| title | Designer substrates to engineer stem cells secretome production |
| title_full | Designer substrates to engineer stem cells secretome production |
| title_fullStr | Designer substrates to engineer stem cells secretome production |
| title_full_unstemmed | Designer substrates to engineer stem cells secretome production |
| title_short | Designer substrates to engineer stem cells secretome production |
| title_sort | designer substrates to engineer stem cells secretome production |
| topic | DRNTU::Engineering::Materials |
| url | http://hdl.handle.net/10356/77270 |
| work_keys_str_mv | AT taysharonweesin designersubstratestoengineerstemcellssecretomeproduction |