Effect of micro-RNA 150 and 885 on liver fibrosis
This paper analyzes the effects of MiRNA 150 and 885 on liver fibrosis. To establish if these MiRNAs are pro-fibrotic or anti-fibrotic, fibrotic expression of HSC Activation and Quiescence Markers are quantified after over-expressing the MiRNAs in LX2 cells. Our results suggest that MiRNA 150 is pro...
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| Format: | Final Year Project (FYP) |
| Language: | English |
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2019
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| Online Access: | http://hdl.handle.net/10356/78035 |
| _version_ | 1826110573757071360 |
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| author | Ali, Ashik |
| author2 | Dang Thuy Tram |
| author_facet | Dang Thuy Tram Ali, Ashik |
| author_sort | Ali, Ashik |
| collection | NTU |
| description | This paper analyzes the effects of MiRNA 150 and 885 on liver fibrosis. To establish if these MiRNAs are pro-fibrotic or anti-fibrotic, fibrotic expression of HSC Activation and Quiescence Markers are quantified after over-expressing the MiRNAs in LX2 cells. Our results suggest that MiRNA 150 is pro-fibrotic due to the relative over-expression of classical HSC activation markers while MiRNA 885 tends to be inconclusive with inconsistent results. This paper also serves as a guide for cloning and testing MiRNAs in cells for any gene expression in-vitro. MiRNA 150 can be further studied on a protein level for potential therapeutic value to those suffering from Liver fibrosis. |
| first_indexed | 2024-10-01T02:36:40Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/78035 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T02:36:40Z |
| publishDate | 2019 |
| record_format | dspace |
| spelling | ntu-10356/780352023-03-03T15:32:10Z Effect of micro-RNA 150 and 885 on liver fibrosis Ali, Ashik Dang Thuy Tram School of Chemical and Biomedical Engineering A*STAR Raymond Ng DRNTU::Science::Biological sciences DRNTU::Engineering::Bioengineering This paper analyzes the effects of MiRNA 150 and 885 on liver fibrosis. To establish if these MiRNAs are pro-fibrotic or anti-fibrotic, fibrotic expression of HSC Activation and Quiescence Markers are quantified after over-expressing the MiRNAs in LX2 cells. Our results suggest that MiRNA 150 is pro-fibrotic due to the relative over-expression of classical HSC activation markers while MiRNA 885 tends to be inconclusive with inconsistent results. This paper also serves as a guide for cloning and testing MiRNAs in cells for any gene expression in-vitro. MiRNA 150 can be further studied on a protein level for potential therapeutic value to those suffering from Liver fibrosis. Bachelor of Engineering (Bioengineering) 2019-06-11T05:33:45Z 2019-06-11T05:33:45Z 2019 Final Year Project (FYP) http://hdl.handle.net/10356/78035 en Nanyang Technological University 46 p. application/pdf |
| spellingShingle | DRNTU::Science::Biological sciences DRNTU::Engineering::Bioengineering Ali, Ashik Effect of micro-RNA 150 and 885 on liver fibrosis |
| title | Effect of micro-RNA 150 and 885 on liver fibrosis |
| title_full | Effect of micro-RNA 150 and 885 on liver fibrosis |
| title_fullStr | Effect of micro-RNA 150 and 885 on liver fibrosis |
| title_full_unstemmed | Effect of micro-RNA 150 and 885 on liver fibrosis |
| title_short | Effect of micro-RNA 150 and 885 on liver fibrosis |
| title_sort | effect of micro rna 150 and 885 on liver fibrosis |
| topic | DRNTU::Science::Biological sciences DRNTU::Engineering::Bioengineering |
| url | http://hdl.handle.net/10356/78035 |
| work_keys_str_mv | AT aliashik effectofmicrorna150and885onliverfibrosis |