Biochip assessment of extended viability and functionality for tissue biopsies
Transepithelial electrical resistance (TEER) is a non-invasive method employed to determine the viability of cells and tissue quantitatively. At present, measuring TEER of explants and tissue continue to pose a challenge both in procedure and cost. To address this, a lower cost microfluidic chip wit...
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| Format: | Final Year Project (FYP) |
| Language: | English |
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2019
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| Online Access: | http://hdl.handle.net/10356/78164 |
| _version_ | 1811695962730528768 |
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| author | Ang, Russell Way Loon |
| author2 | Poenar Daniel Puiu |
| author_facet | Poenar Daniel Puiu Ang, Russell Way Loon |
| author_sort | Ang, Russell Way Loon |
| collection | NTU |
| description | Transepithelial electrical resistance (TEER) is a non-invasive method employed to determine the viability of cells and tissue quantitatively. At present, measuring TEER of explants and tissue continue to pose a challenge both in procedure and cost. To address this, a lower cost microfluidic chip with two perfusion channels for dynamic medium flow, that can continuously measure TEER, was developed using polydimethylsiloxane (PDMS) and Poly (methyl methacrylate) (PMMA). After which, the chips were tested using pig epithelial esophageal tissue and mice gastrointestinal track tissue. The experiments conducted showed that the chip was able to maintain the viability of the tissue, albeit not as effective as off-chip methods due to the lack of oxygen, and measure TEER as accurately as off-chip methods despite background resistance. With further research, the problem with the lack of oxygen can be resolved and the fabrication of the chip can be made more cost effective and commercially viable. |
| first_indexed | 2024-10-01T07:31:48Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/78164 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T07:31:48Z |
| publishDate | 2019 |
| record_format | dspace |
| spelling | ntu-10356/781642023-07-07T17:38:06Z Biochip assessment of extended viability and functionality for tissue biopsies Ang, Russell Way Loon Poenar Daniel Puiu School of Electrical and Electronic Engineering DRNTU::Engineering::Electrical and electronic engineering Transepithelial electrical resistance (TEER) is a non-invasive method employed to determine the viability of cells and tissue quantitatively. At present, measuring TEER of explants and tissue continue to pose a challenge both in procedure and cost. To address this, a lower cost microfluidic chip with two perfusion channels for dynamic medium flow, that can continuously measure TEER, was developed using polydimethylsiloxane (PDMS) and Poly (methyl methacrylate) (PMMA). After which, the chips were tested using pig epithelial esophageal tissue and mice gastrointestinal track tissue. The experiments conducted showed that the chip was able to maintain the viability of the tissue, albeit not as effective as off-chip methods due to the lack of oxygen, and measure TEER as accurately as off-chip methods despite background resistance. With further research, the problem with the lack of oxygen can be resolved and the fabrication of the chip can be made more cost effective and commercially viable. Bachelor of Engineering (Electrical and Electronic Engineering) 2019-06-13T01:39:16Z 2019-06-13T01:39:16Z 2019 Final Year Project (FYP) http://hdl.handle.net/10356/78164 en Nanyang Technological University 61 p. application/pdf |
| spellingShingle | DRNTU::Engineering::Electrical and electronic engineering Ang, Russell Way Loon Biochip assessment of extended viability and functionality for tissue biopsies |
| title | Biochip assessment of extended viability and functionality for tissue biopsies |
| title_full | Biochip assessment of extended viability and functionality for tissue biopsies |
| title_fullStr | Biochip assessment of extended viability and functionality for tissue biopsies |
| title_full_unstemmed | Biochip assessment of extended viability and functionality for tissue biopsies |
| title_short | Biochip assessment of extended viability and functionality for tissue biopsies |
| title_sort | biochip assessment of extended viability and functionality for tissue biopsies |
| topic | DRNTU::Engineering::Electrical and electronic engineering |
| url | http://hdl.handle.net/10356/78164 |
| work_keys_str_mv | AT angrussellwayloon biochipassessmentofextendedviabilityandfunctionalityfortissuebiopsies |