Identification and validation of immune correlates of protection against malaria infection
Current intervening approaches against malaria are insufficient to eradicate malaria. Hence, a new vaccine which can induce long-lasting sterile protection against malaria needs to be developed. Immunisation with live sporozoites under mefloquine or chloroquine drug cover can induce protection in hu...
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| Format: | Final Year Project (FYP) |
| Language: | English |
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2019
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| Online Access: | http://hdl.handle.net/10356/78850 |
| _version_ | 1824456401716510720 |
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| author | Lee, Qian Qi |
| author2 | Laurent Rénia |
| author_facet | Laurent Rénia Lee, Qian Qi |
| author_sort | Lee, Qian Qi |
| collection | NTU |
| description | Current intervening approaches against malaria are insufficient to eradicate malaria. Hence, a new vaccine which can induce long-lasting sterile protection against malaria needs to be developed. Immunisation with live sporozoites under mefloquine or chloroquine drug cover can induce protection in human volunteers who were challenged with sporozoites experimentally. We compared between the two differential sets of serum, taken from protected and non-protected individuals who had undergone the immunisation regime, to identify antigens which could be used for vaccine development. We screened 7 antigens and 5 of the antigens screened were not associated with protection. In the chloroquine chemoprophylaxis group, serum response was observed in 1/3 protected volunteers and none in the non-protected volunteers for blood stage antigens MSP2 and RHOPH3. Further works need to be done to validate the correlation between MSP2 and RHOPH3 with protection due to the small sample size. |
| first_indexed | 2025-02-19T03:53:31Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/78850 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2025-02-19T03:53:31Z |
| publishDate | 2019 |
| record_format | dspace |
| spelling | ntu-10356/788502023-02-28T18:06:08Z Identification and validation of immune correlates of protection against malaria infection Lee, Qian Qi Laurent Rénia School of Biological Sciences Goh Yun Shan Science::Biological sciences Current intervening approaches against malaria are insufficient to eradicate malaria. Hence, a new vaccine which can induce long-lasting sterile protection against malaria needs to be developed. Immunisation with live sporozoites under mefloquine or chloroquine drug cover can induce protection in human volunteers who were challenged with sporozoites experimentally. We compared between the two differential sets of serum, taken from protected and non-protected individuals who had undergone the immunisation regime, to identify antigens which could be used for vaccine development. We screened 7 antigens and 5 of the antigens screened were not associated with protection. In the chloroquine chemoprophylaxis group, serum response was observed in 1/3 protected volunteers and none in the non-protected volunteers for blood stage antigens MSP2 and RHOPH3. Further works need to be done to validate the correlation between MSP2 and RHOPH3 with protection due to the small sample size. Bachelor of Science in Biological Sciences 2019-08-05T01:04:48Z 2019-08-05T01:04:48Z 2019 Final Year Project (FYP) http://hdl.handle.net/10356/78850 en Nanyang Technological University 29 p. application/pdf |
| spellingShingle | Science::Biological sciences Lee, Qian Qi Identification and validation of immune correlates of protection against malaria infection |
| title | Identification and validation of immune correlates of protection against malaria infection |
| title_full | Identification and validation of immune correlates of protection against malaria infection |
| title_fullStr | Identification and validation of immune correlates of protection against malaria infection |
| title_full_unstemmed | Identification and validation of immune correlates of protection against malaria infection |
| title_short | Identification and validation of immune correlates of protection against malaria infection |
| title_sort | identification and validation of immune correlates of protection against malaria infection |
| topic | Science::Biological sciences |
| url | http://hdl.handle.net/10356/78850 |
| work_keys_str_mv | AT leeqianqi identificationandvalidationofimmunecorrelatesofprotectionagainstmalariainfection |