An essential role for maternal control of Nodal signaling
Growth factor signaling is essential for pattern formation, growth, differentiation, and maintenance of stem cell pluripotency. Nodal-related signaling factors are required for axis formation and germ layer specification from sea urchins to mammals. Maternal transcripts of the zebrafish Nodal factor...
| Main Authors: | , , , , , , |
|---|---|
| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2013
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/79319 http://hdl.handle.net/10220/17445 |
| _version_ | 1824453558645293056 |
|---|---|
| author | Tran, Long Duc Kumari, Pooja Gilligan, Patrick C. Lim, Shimin Winkler, Sylke Philp, Robin Sampath, Karuna |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Tran, Long Duc Kumari, Pooja Gilligan, Patrick C. Lim, Shimin Winkler, Sylke Philp, Robin Sampath, Karuna |
| author_sort | Tran, Long Duc |
| collection | NTU |
| description | Growth factor signaling is essential for pattern formation, growth, differentiation, and maintenance of stem cell pluripotency. Nodal-related signaling factors are required for axis formation and germ layer specification from sea urchins to mammals. Maternal transcripts of the zebrafish Nodal factor, Squint (Sqt), are localized to future embryonic dorsal. The mechanisms by which maternal sqt/nodal RNA is localized and regulated have been unclear. Here, we show that maternal control of Nodal signaling via the conserved Y box-binding protein 1 (Ybx1) is essential. We identified Ybx1 via a proteomic screen. Ybx1 recognizes the 3’ untranslated region (UTR) of sqt RNA and prevents premature translation and Sqt/Nodal signaling. Maternal-effect mutations in zebrafish ybx1 lead to deregulated Nodal signaling, gastrulation failure, and embryonic lethality. Implanted Nodal-coated beads phenocopy ybx1 mutant defects. Thus, Ybx1 prevents ectopic Nodal activity, revealing a new paradigm in the regulation of Nodal signaling, which is likely to be conserved. |
| first_indexed | 2025-02-19T03:08:20Z |
| format | Journal Article |
| id | ntu-10356/79319 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2025-02-19T03:08:20Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | ntu-10356/793192023-02-28T16:58:46Z An essential role for maternal control of Nodal signaling Tran, Long Duc Kumari, Pooja Gilligan, Patrick C. Lim, Shimin Winkler, Sylke Philp, Robin Sampath, Karuna School of Biological Sciences DRNTU::Science::Biological sciences Growth factor signaling is essential for pattern formation, growth, differentiation, and maintenance of stem cell pluripotency. Nodal-related signaling factors are required for axis formation and germ layer specification from sea urchins to mammals. Maternal transcripts of the zebrafish Nodal factor, Squint (Sqt), are localized to future embryonic dorsal. The mechanisms by which maternal sqt/nodal RNA is localized and regulated have been unclear. Here, we show that maternal control of Nodal signaling via the conserved Y box-binding protein 1 (Ybx1) is essential. We identified Ybx1 via a proteomic screen. Ybx1 recognizes the 3’ untranslated region (UTR) of sqt RNA and prevents premature translation and Sqt/Nodal signaling. Maternal-effect mutations in zebrafish ybx1 lead to deregulated Nodal signaling, gastrulation failure, and embryonic lethality. Implanted Nodal-coated beads phenocopy ybx1 mutant defects. Thus, Ybx1 prevents ectopic Nodal activity, revealing a new paradigm in the regulation of Nodal signaling, which is likely to be conserved. ASTAR (Agency for Sci., Tech. and Research, S’pore) Published Version 2013-11-08T04:26:20Z 2019-12-06T13:22:27Z 2013-11-08T04:26:20Z 2019-12-06T13:22:27Z 2013 2013 Journal Article Kumari, P., Gilligan, P. C., Lim, S., Tran, L. D., Winkler, S., Philp, R., et al. (2013). An essential role for maternal control of Nodal signaling. eLife, 2, e00683-. 2050-084X https://hdl.handle.net/10356/79319 http://hdl.handle.net/10220/17445 10.7554/eLife.00683 24040511 en eLife © 2013 Kumari et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited. application/pdf |
| spellingShingle | DRNTU::Science::Biological sciences Tran, Long Duc Kumari, Pooja Gilligan, Patrick C. Lim, Shimin Winkler, Sylke Philp, Robin Sampath, Karuna An essential role for maternal control of Nodal signaling |
| title | An essential role for maternal control of Nodal signaling |
| title_full | An essential role for maternal control of Nodal signaling |
| title_fullStr | An essential role for maternal control of Nodal signaling |
| title_full_unstemmed | An essential role for maternal control of Nodal signaling |
| title_short | An essential role for maternal control of Nodal signaling |
| title_sort | essential role for maternal control of nodal signaling |
| topic | DRNTU::Science::Biological sciences |
| url | https://hdl.handle.net/10356/79319 http://hdl.handle.net/10220/17445 |
| work_keys_str_mv | AT tranlongduc anessentialroleformaternalcontrolofnodalsignaling AT kumaripooja anessentialroleformaternalcontrolofnodalsignaling AT gilliganpatrickc anessentialroleformaternalcontrolofnodalsignaling AT limshimin anessentialroleformaternalcontrolofnodalsignaling AT winklersylke anessentialroleformaternalcontrolofnodalsignaling AT philprobin anessentialroleformaternalcontrolofnodalsignaling AT sampathkaruna anessentialroleformaternalcontrolofnodalsignaling AT tranlongduc essentialroleformaternalcontrolofnodalsignaling AT kumaripooja essentialroleformaternalcontrolofnodalsignaling AT gilliganpatrickc essentialroleformaternalcontrolofnodalsignaling AT limshimin essentialroleformaternalcontrolofnodalsignaling AT winklersylke essentialroleformaternalcontrolofnodalsignaling AT philprobin essentialroleformaternalcontrolofnodalsignaling AT sampathkaruna essentialroleformaternalcontrolofnodalsignaling |