Chemical mediation of coral larval settlement by crustose coralline algae
The majority of marine invertebrates produce dispersive larvae which, in order to complete their life cycles, must attach and metamorphose into benthic forms. This process, collectively referred to as settlement, is often guided by habitat-specific cues. While the sources of such cues are well known...
| Main Authors: | , , , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
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2015
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| Online Access: | https://hdl.handle.net/10356/79454 http://hdl.handle.net/10220/26061 |
| _version_ | 1824455479105945600 |
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| author | Tebben, J. Motti, C. A Siboni, Nachshon Negri, A. P. Harder, T. Steinberg, Peter David Hatta, Masayuki Tapiolas, D. M. Schupp, P. J. Kitamura, Makoto |
| author2 | Advanced Environmental Biotechnology Centre (AEBC) |
| author_facet | Advanced Environmental Biotechnology Centre (AEBC) Tebben, J. Motti, C. A Siboni, Nachshon Negri, A. P. Harder, T. Steinberg, Peter David Hatta, Masayuki Tapiolas, D. M. Schupp, P. J. Kitamura, Makoto |
| author_sort | Tebben, J. |
| collection | NTU |
| description | The majority of marine invertebrates produce dispersive larvae which, in order to complete their life cycles, must attach and metamorphose into benthic forms. This process, collectively referred to as settlement, is often guided by habitat-specific cues. While the sources of such cues are well known, the links between their biological activity, chemical identity, presence and quantification in situ are largely missing. Previous work on coral larval settlement in vitro has shown widespread induction by crustose coralline algae (CCA) and in particular their associated bacteria. However, we found that bacterial biofilms on CCA did not initiate ecologically realistic settlement responses in larvae of 11 hard coral species from Australia, Guam, Singapore and Japan. We instead found that algal chemical cues induce identical behavioral responses of larvae as per live CCA. We identified two classes of CCA cell wall-associated compounds – glycoglycerolipids and polysaccharides – as the main constituents of settlement inducing fractions. These algae-derived fractions induce settlement and metamorphosis at equivalent concentrations as present in CCA, both in small scale laboratory assays and under flow-through conditions, suggesting their ability to act in an ecologically relevant fashion to steer larval settlement of corals. Both compound classes were readily detected in natural samples. |
| first_indexed | 2025-02-19T03:38:51Z |
| format | Journal Article |
| id | ntu-10356/79454 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2025-02-19T03:38:51Z |
| publishDate | 2015 |
| record_format | dspace |
| spelling | ntu-10356/794542022-02-16T16:30:57Z Chemical mediation of coral larval settlement by crustose coralline algae Tebben, J. Motti, C. A Siboni, Nachshon Negri, A. P. Harder, T. Steinberg, Peter David Hatta, Masayuki Tapiolas, D. M. Schupp, P. J. Kitamura, Makoto Advanced Environmental Biotechnology Centre (AEBC) Nanyang Environment and Water Research Institute DRNTU::Science::Biological sciences The majority of marine invertebrates produce dispersive larvae which, in order to complete their life cycles, must attach and metamorphose into benthic forms. This process, collectively referred to as settlement, is often guided by habitat-specific cues. While the sources of such cues are well known, the links between their biological activity, chemical identity, presence and quantification in situ are largely missing. Previous work on coral larval settlement in vitro has shown widespread induction by crustose coralline algae (CCA) and in particular their associated bacteria. However, we found that bacterial biofilms on CCA did not initiate ecologically realistic settlement responses in larvae of 11 hard coral species from Australia, Guam, Singapore and Japan. We instead found that algal chemical cues induce identical behavioral responses of larvae as per live CCA. We identified two classes of CCA cell wall-associated compounds – glycoglycerolipids and polysaccharides – as the main constituents of settlement inducing fractions. These algae-derived fractions induce settlement and metamorphosis at equivalent concentrations as present in CCA, both in small scale laboratory assays and under flow-through conditions, suggesting their ability to act in an ecologically relevant fashion to steer larval settlement of corals. Both compound classes were readily detected in natural samples. Published version 2015-06-25T02:00:43Z 2019-12-06T13:25:45Z 2015-06-25T02:00:43Z 2019-12-06T13:25:45Z 2015 2015 Journal Article Tebben, J., Motti, C. A., Siboni, N., Tapiolas, D. M., Negri, A. P., Schupp, P. J., et al. (2015). Chemical mediation of coral larval settlement by crustose coralline algae. Scientific Reports, 5, 10803-. 2045-2322 https://hdl.handle.net/10356/79454 http://hdl.handle.net/10220/26061 10.1038/srep10803 26042834 en Scientific Reports This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ application/pdf |
| spellingShingle | DRNTU::Science::Biological sciences Tebben, J. Motti, C. A Siboni, Nachshon Negri, A. P. Harder, T. Steinberg, Peter David Hatta, Masayuki Tapiolas, D. M. Schupp, P. J. Kitamura, Makoto Chemical mediation of coral larval settlement by crustose coralline algae |
| title | Chemical mediation of coral larval settlement by crustose coralline algae |
| title_full | Chemical mediation of coral larval settlement by crustose coralline algae |
| title_fullStr | Chemical mediation of coral larval settlement by crustose coralline algae |
| title_full_unstemmed | Chemical mediation of coral larval settlement by crustose coralline algae |
| title_short | Chemical mediation of coral larval settlement by crustose coralline algae |
| title_sort | chemical mediation of coral larval settlement by crustose coralline algae |
| topic | DRNTU::Science::Biological sciences |
| url | https://hdl.handle.net/10356/79454 http://hdl.handle.net/10220/26061 |
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