Detection of Listeria monocytogenes by using the polymerase chain reaction
A method was developed for detection of Listeria monocytogenes by polymerase chain reaction amplification followed by agarose gel electrophoresis or dot blot analysis with a 32P-labeled internal probe. The technique identified 95 of 95 L. monocytogenes strains, 0 of 12 Listeria strains of other spec...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2014
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/79472 http://hdl.handle.net/10220/18733 http://aem.asm.org/content/56/9/2930.full.pdf+html |
| _version_ | 1826112616951447552 |
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| author | Rotbart, Harley A. Blaser, Martin J. Ellison III, Richard T. Bessesen, Mary T. Luo, Qian |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Rotbart, Harley A. Blaser, Martin J. Ellison III, Richard T. Bessesen, Mary T. Luo, Qian |
| author_sort | Rotbart, Harley A. |
| collection | NTU |
| description | A method was developed for detection of Listeria monocytogenes by polymerase chain reaction amplification followed by agarose gel electrophoresis or dot blot analysis with a 32P-labeled internal probe. The technique identified 95 of 95 L. monocytogenes strains, 0 of 12 Listeria strains of other species, and 0 of 12 non-Listeria strains. |
| first_indexed | 2024-10-01T03:10:00Z |
| format | Journal Article |
| id | ntu-10356/79472 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:10:00Z |
| publishDate | 2014 |
| record_format | dspace |
| spelling | ntu-10356/794722023-12-29T06:46:13Z Detection of Listeria monocytogenes by using the polymerase chain reaction Rotbart, Harley A. Blaser, Martin J. Ellison III, Richard T. Bessesen, Mary T. Luo, Qian School of Chemical and Biomedical Engineering DRNTU::Engineering::Chemical engineering::Chemical processes A method was developed for detection of Listeria monocytogenes by polymerase chain reaction amplification followed by agarose gel electrophoresis or dot blot analysis with a 32P-labeled internal probe. The technique identified 95 of 95 L. monocytogenes strains, 0 of 12 Listeria strains of other species, and 0 of 12 non-Listeria strains. Accepted version 2014-01-29T01:54:50Z 2019-12-06T13:26:09Z 2014-01-29T01:54:50Z 2019-12-06T13:26:09Z 1990 1990 Journal Article Bessesen, M. T., Luo, Q., Rotbart, H. A., Blaser, M. J., & Ellison III, R. T. (1990). Detection of Listeria monocytogenes by using the polymerase chain reaction. Applied and environmental microbiology, 56(9), 2930-2932. https://hdl.handle.net/10356/79472 http://hdl.handle.net/10220/18733 http://aem.asm.org/content/56/9/2930.full.pdf+html en Applied and environmental microbiology © 1990 American Society for Microbiology. This is the author created version of a work that has been peer reviewed and accepted for publication by Applied And Environmental Microbiology, American Society for Microbiology. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://aem.asm.org/content/56/9/2930.full.pdf+html]. 3 p. application/pdf |
| spellingShingle | DRNTU::Engineering::Chemical engineering::Chemical processes Rotbart, Harley A. Blaser, Martin J. Ellison III, Richard T. Bessesen, Mary T. Luo, Qian Detection of Listeria monocytogenes by using the polymerase chain reaction |
| title | Detection of Listeria monocytogenes by using the polymerase chain reaction |
| title_full | Detection of Listeria monocytogenes by using the polymerase chain reaction |
| title_fullStr | Detection of Listeria monocytogenes by using the polymerase chain reaction |
| title_full_unstemmed | Detection of Listeria monocytogenes by using the polymerase chain reaction |
| title_short | Detection of Listeria monocytogenes by using the polymerase chain reaction |
| title_sort | detection of listeria monocytogenes by using the polymerase chain reaction |
| topic | DRNTU::Engineering::Chemical engineering::Chemical processes |
| url | https://hdl.handle.net/10356/79472 http://hdl.handle.net/10220/18733 http://aem.asm.org/content/56/9/2930.full.pdf+html |
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