Identification of a new export signal in Plasmodium yoelii : identification of a new exportome
Development of the erythrocytic malaria parasite requires targeting of parasite proteins into multiple compartments located within and beyond the parasite confine. Beyond the PEXEL/VTS pathway and its characterized players, increasing amount of evidence has highlighted the existence of proteins expo...
| Main Authors: | , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
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2015
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| Online Access: | https://hdl.handle.net/10356/79474 http://hdl.handle.net/10220/25110 |
| _version_ | 1811689182509137920 |
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| author | Siau, Anthony Huang, Ximei Yam, Xue Yan Bob, Ndeye Sakha Sun, Hequan Rajapakse, Jagath C. Renia, Laurent Preiser, Peter R. |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Siau, Anthony Huang, Ximei Yam, Xue Yan Bob, Ndeye Sakha Sun, Hequan Rajapakse, Jagath C. Renia, Laurent Preiser, Peter R. |
| author_sort | Siau, Anthony |
| collection | NTU |
| description | Development of the erythrocytic malaria parasite requires targeting of parasite proteins into multiple compartments located within and beyond the parasite confine. Beyond the PEXEL/VTS pathway and its characterized players, increasing amount of evidence has highlighted the existence of proteins exported using alternative export-signal(s)/pathway(s); hence, the exportomes currently predicted are incomplete. The nature of these exported proteins which could have a prominent role in most of the Plasmodium species remains elusive. Using P. yoelii variant proteins, we identified a signal associated to lipophilic region that mediates export of P. yoelii proteins. This non-PEXEL signal termed PLASMED is defined by semi-conserved residues and possibly a secondary structure. In vivo characterization of exported-proteins indicated that PLASMED is a bona fide export-signal that allowed us to identify an unseen P. yoelii exportome. The repertoire of the newly predicted exported proteins opens up perspectives for unravelling the remodelling of the host-cell by the parasite, against which new therapies could be elaborated. |
| first_indexed | 2024-10-01T05:44:02Z |
| format | Journal Article |
| id | ntu-10356/79474 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T05:44:02Z |
| publishDate | 2015 |
| record_format | dspace |
| spelling | ntu-10356/794742023-02-28T16:58:22Z Identification of a new export signal in Plasmodium yoelii : identification of a new exportome Siau, Anthony Huang, Ximei Yam, Xue Yan Bob, Ndeye Sakha Sun, Hequan Rajapakse, Jagath C. Renia, Laurent Preiser, Peter R. School of Biological Sciences DRNTU::Science::Biological sciences::Microbiology Development of the erythrocytic malaria parasite requires targeting of parasite proteins into multiple compartments located within and beyond the parasite confine. Beyond the PEXEL/VTS pathway and its characterized players, increasing amount of evidence has highlighted the existence of proteins exported using alternative export-signal(s)/pathway(s); hence, the exportomes currently predicted are incomplete. The nature of these exported proteins which could have a prominent role in most of the Plasmodium species remains elusive. Using P. yoelii variant proteins, we identified a signal associated to lipophilic region that mediates export of P. yoelii proteins. This non-PEXEL signal termed PLASMED is defined by semi-conserved residues and possibly a secondary structure. In vivo characterization of exported-proteins indicated that PLASMED is a bona fide export-signal that allowed us to identify an unseen P. yoelii exportome. The repertoire of the newly predicted exported proteins opens up perspectives for unravelling the remodelling of the host-cell by the parasite, against which new therapies could be elaborated. Accepted version 2015-02-26T03:56:27Z 2019-12-06T13:26:13Z 2015-02-26T03:56:27Z 2019-12-06T13:26:13Z 2014 2014 Journal Article Siau, A., Huang, X., Yam, X. Y., Bob, N. S., Sun, H., Rajapakse, J. C., et al. (2014). Identification of a new export signal in Plasmodium yoelii : identification of a new exportome. Cellular microbiology, 16(5), 673-686. 1462-5814 https://hdl.handle.net/10356/79474 http://hdl.handle.net/10220/25110 10.1111/cmi.12293 en Cellular microbiology © 2014 John Wiley & Sons Ltd. This is the author created version of a work that has been peer reviewed and accepted for publication by Cellular Microbiology, John Wiley & Sons Ltd. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [Article URL/DOI: http://dx.doi.org/10.1111/cmi.12293]. 33 p. application/pdf |
| spellingShingle | DRNTU::Science::Biological sciences::Microbiology Siau, Anthony Huang, Ximei Yam, Xue Yan Bob, Ndeye Sakha Sun, Hequan Rajapakse, Jagath C. Renia, Laurent Preiser, Peter R. Identification of a new export signal in Plasmodium yoelii : identification of a new exportome |
| title | Identification of a new export signal in Plasmodium yoelii : identification of a new exportome |
| title_full | Identification of a new export signal in Plasmodium yoelii : identification of a new exportome |
| title_fullStr | Identification of a new export signal in Plasmodium yoelii : identification of a new exportome |
| title_full_unstemmed | Identification of a new export signal in Plasmodium yoelii : identification of a new exportome |
| title_short | Identification of a new export signal in Plasmodium yoelii : identification of a new exportome |
| title_sort | identification of a new export signal in plasmodium yoelii identification of a new exportome |
| topic | DRNTU::Science::Biological sciences::Microbiology |
| url | https://hdl.handle.net/10356/79474 http://hdl.handle.net/10220/25110 |
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