Melanoma-initiating cells exploit M2 macrophage TGFβ and arginase pathway for survival and proliferation
M2 macrophages promote tumor growth and metastasis, but their interactions with specific tumor cell populations are poorly characterized. Using a mouse model of spontaneous melanoma, we showed that CD34- but not CD34+ tumor-initiating cells (TICs) depend on M2 macrophages for survival and proliferat...
| Main Authors: | , , , , , , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2015
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| Online Access: | https://hdl.handle.net/10356/79494 http://hdl.handle.net/10220/24702 http://www.impactjournals.com/oncotarget/index.php?journal=oncotarget&page=article&op=view&path[]=2482 |
| _version_ | 1811687298520055808 |
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| author | Tan, Kar Wai Kato, Masashi Keeble, Jo Wang, Xiaojie Hubert, Sandra Barron, Luke Tan, Nguan Soon Angeli, Veronique Abastado, Jean-Pierre Tham, Muly Prevost-Blondel, Armelle |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Tan, Kar Wai Kato, Masashi Keeble, Jo Wang, Xiaojie Hubert, Sandra Barron, Luke Tan, Nguan Soon Angeli, Veronique Abastado, Jean-Pierre Tham, Muly Prevost-Blondel, Armelle |
| author_sort | Tan, Kar Wai |
| collection | NTU |
| description | M2 macrophages promote tumor growth and metastasis, but their interactions with specific tumor cell populations are poorly characterized. Using a mouse model of spontaneous melanoma, we showed that CD34- but not CD34+ tumor-initiating cells (TICs) depend on M2 macrophages for survival and proliferation. Tumor-associated macrophages (TAMs) and macrophage-conditioned media protected CD34- TICs from chemotherapy in vitro. In vivo, while inhibition of CD115 suppressed the macrophage-dependent CD34- TIC population, chemotherapy accelerated its development. The ability of TICs to respond to TAMs was acquired during melanoma progression and immediately preceded a surge in metastatic outgrowth. TAM-derived transforming growth factor-β (TGFβ) and polyamines produced via the Arginase pathway were critical for stimulation of TICs and synergized to promote their growth. |
| first_indexed | 2024-10-01T05:14:05Z |
| format | Journal Article |
| id | ntu-10356/79494 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T05:14:05Z |
| publishDate | 2015 |
| record_format | dspace |
| spelling | ntu-10356/794942023-02-28T16:58:31Z Melanoma-initiating cells exploit M2 macrophage TGFβ and arginase pathway for survival and proliferation Tan, Kar Wai Kato, Masashi Keeble, Jo Wang, Xiaojie Hubert, Sandra Barron, Luke Tan, Nguan Soon Angeli, Veronique Abastado, Jean-Pierre Tham, Muly Prevost-Blondel, Armelle School of Biological Sciences DRNTU::Science::Biological sciences M2 macrophages promote tumor growth and metastasis, but their interactions with specific tumor cell populations are poorly characterized. Using a mouse model of spontaneous melanoma, we showed that CD34- but not CD34+ tumor-initiating cells (TICs) depend on M2 macrophages for survival and proliferation. Tumor-associated macrophages (TAMs) and macrophage-conditioned media protected CD34- TICs from chemotherapy in vitro. In vivo, while inhibition of CD115 suppressed the macrophage-dependent CD34- TIC population, chemotherapy accelerated its development. The ability of TICs to respond to TAMs was acquired during melanoma progression and immediately preceded a surge in metastatic outgrowth. TAM-derived transforming growth factor-β (TGFβ) and polyamines produced via the Arginase pathway were critical for stimulation of TICs and synergized to promote their growth. Published version 2015-01-20T08:00:16Z 2019-12-06T13:26:42Z 2015-01-20T08:00:16Z 2019-12-06T13:26:42Z 2014 2014 Journal Article Tham, M., Tan, K. W., Keeble, Jo., Wang, X., Hubert, S., Barron, L., et al. (2014). Melanoma-initiating cells exploit M2 macrophage TGFβ and arginase pathway for survival and proliferation. Oncotarget, 5(23), 12027-12042. 1949-2553 https://hdl.handle.net/10356/79494 http://hdl.handle.net/10220/24702 http://www.impactjournals.com/oncotarget/index.php?journal=oncotarget&page=article&op=view&path[]=2482 en Oncotarget This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 16 p. application/pdf |
| spellingShingle | DRNTU::Science::Biological sciences Tan, Kar Wai Kato, Masashi Keeble, Jo Wang, Xiaojie Hubert, Sandra Barron, Luke Tan, Nguan Soon Angeli, Veronique Abastado, Jean-Pierre Tham, Muly Prevost-Blondel, Armelle Melanoma-initiating cells exploit M2 macrophage TGFβ and arginase pathway for survival and proliferation |
| title | Melanoma-initiating cells exploit M2 macrophage TGFβ and arginase pathway for survival and proliferation |
| title_full | Melanoma-initiating cells exploit M2 macrophage TGFβ and arginase pathway for survival and proliferation |
| title_fullStr | Melanoma-initiating cells exploit M2 macrophage TGFβ and arginase pathway for survival and proliferation |
| title_full_unstemmed | Melanoma-initiating cells exploit M2 macrophage TGFβ and arginase pathway for survival and proliferation |
| title_short | Melanoma-initiating cells exploit M2 macrophage TGFβ and arginase pathway for survival and proliferation |
| title_sort | melanoma initiating cells exploit m2 macrophage tgfβ and arginase pathway for survival and proliferation |
| topic | DRNTU::Science::Biological sciences |
| url | https://hdl.handle.net/10356/79494 http://hdl.handle.net/10220/24702 http://www.impactjournals.com/oncotarget/index.php?journal=oncotarget&page=article&op=view&path[]=2482 |
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