Label-free, electrochemical detection of methicillin-resistant staphylococcus aureus DNA with reduced graphene oxide-modified electrodes

Label-free, electrochemical detection of methicillin-resistant staphylococcus aureus DNA with reduced graphene oxide-modified electrodes

Reduced graphene oxide (rGO)-modified glassy carbon electrode is used to detect the methicillinresistant Staphylococcus aureus (MRSA) DNA by using electrochemical impedance spectroscopy. Our experiments confirm that ssDNA, before and after hybridization with target DNA, are successfully anchored on...

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Bibliografiska uppgifter
Huvudupphovsmän: Wang, Zhijuan, Zhang, Juan, Chen, Peng, Zhou, Xiaozhu, Yang, Yanli, Wu, Shixin, Han, Yu, Wang, Lianhui, Boey, Freddy Yin Chiang, Niu, Li, Zhang, Qichun, Bo, Liedberg, Zhang, Hua
Övriga upphovsmän: School of Materials Science & Engineering
Materialtyp: Journal Article
Språk:English
Publicerad: 2012
Ämnen:
DRNTU::Science::Medicine::Biosensors
Länkar:https://hdl.handle.net/10356/79512
http://hdl.handle.net/10220/8333
Beskrivning
Sammanfattning:Reduced graphene oxide (rGO)-modified glassy carbon electrode is used to detect the methicillinresistant Staphylococcus aureus (MRSA) DNA by using electrochemical impedance spectroscopy. Our experiments confirm that ssDNA, before and after hybridization with target DNA, are successfully anchored on the rGO surface. After the probe DNA, pre-adsorbed on rGO electrode, hybridizes with target DNA, the measured impedance increases dramatically. It provides a new method to detect DNA with high sensitivity (10−13 M, i.e., 100 fM) and selectivity.

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