Label-free, electrochemical detection of methicillin-resistant staphylococcus aureus DNA with reduced graphene oxide-modified electrodes
Reduced graphene oxide (rGO)-modified glassy carbon electrode is used to detect the methicillinresistant Staphylococcus aureus (MRSA) DNA by using electrochemical impedance spectroscopy. Our experiments confirm that ssDNA, before and after hybridization with target DNA, are successfully anchored on...
| Main Authors: | , , , , , , , , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2012
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/79512 http://hdl.handle.net/10220/8333 |
| _version_ | 1811676825919684608 |
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| author | Wang, Zhijuan Zhang, Juan Chen, Peng Zhou, Xiaozhu Yang, Yanli Wu, Shixin Han, Yu Wang, Lianhui Chen, Peng Boey, Freddy Yin Chiang Niu, Li Zhang, Qichun Bo, Liedberg Zhang, Hua |
| author2 | School of Materials Science & Engineering |
| author_facet | School of Materials Science & Engineering Wang, Zhijuan Zhang, Juan Chen, Peng Zhou, Xiaozhu Yang, Yanli Wu, Shixin Han, Yu Wang, Lianhui Chen, Peng Boey, Freddy Yin Chiang Niu, Li Zhang, Qichun Bo, Liedberg Zhang, Hua |
| author_sort | Wang, Zhijuan |
| collection | NTU |
| description | Reduced graphene oxide (rGO)-modified glassy carbon electrode is used to detect the methicillinresistant Staphylococcus aureus (MRSA) DNA by using electrochemical impedance spectroscopy. Our experiments confirm that ssDNA, before and after hybridization with target DNA, are successfully anchored on the rGO surface. After the probe DNA, pre-adsorbed on rGO electrode, hybridizes with target DNA, the measured impedance increases dramatically. It provides a new method to detect DNA with high sensitivity (10−13 M, i.e., 100 fM) and selectivity. |
| first_indexed | 2024-10-01T02:27:38Z |
| format | Journal Article |
| id | ntu-10356/79512 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T02:27:38Z |
| publishDate | 2012 |
| record_format | dspace |
| spelling | ntu-10356/795122023-07-14T15:49:29Z Label-free, electrochemical detection of methicillin-resistant staphylococcus aureus DNA with reduced graphene oxide-modified electrodes Wang, Zhijuan Zhang, Juan Chen, Peng Zhou, Xiaozhu Yang, Yanli Wu, Shixin Han, Yu Wang, Lianhui Chen, Peng Boey, Freddy Yin Chiang Niu, Li Zhang, Qichun Bo, Liedberg Zhang, Hua School of Materials Science & Engineering DRNTU::Science::Medicine::Biosensors Reduced graphene oxide (rGO)-modified glassy carbon electrode is used to detect the methicillinresistant Staphylococcus aureus (MRSA) DNA by using electrochemical impedance spectroscopy. Our experiments confirm that ssDNA, before and after hybridization with target DNA, are successfully anchored on the rGO surface. After the probe DNA, pre-adsorbed on rGO electrode, hybridizes with target DNA, the measured impedance increases dramatically. It provides a new method to detect DNA with high sensitivity (10−13 M, i.e., 100 fM) and selectivity. Accepted version 2012-07-13T04:37:09Z 2019-12-06T13:27:09Z 2012-07-13T04:37:09Z 2019-12-06T13:27:09Z 2011 2011 Journal Article Wang, Z., Zhang, J., Chen, P., Zhou, X., Yang, Y., Wu, S., Niu, L., Han, Y., Wang, L., Chen, P., Boey, F., Zhang, Q., Liedberg, B., & Zhang, H. (2011). Label-free, Electrochemical Detection of Methicillin-Resistant Staphylococcus Aureus DNA with Reduced Graphene Oxide-Modified Electrodes. Biosensors and Bioelectronics, 26(9), 3881–3886. https://hdl.handle.net/10356/79512 http://hdl.handle.net/10220/8333 10.1016/j.bios.2011.03.002 en Biosensors and Bioelectronics © 2011 Elsevier B.V. This is the author created version of a work that has been peer reviewed and accepted for publication by Biosensors and Bioelectronics, Elsevier. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1016/j.bios.2011.03.002]. application/pdf |
| spellingShingle | DRNTU::Science::Medicine::Biosensors Wang, Zhijuan Zhang, Juan Chen, Peng Zhou, Xiaozhu Yang, Yanli Wu, Shixin Han, Yu Wang, Lianhui Chen, Peng Boey, Freddy Yin Chiang Niu, Li Zhang, Qichun Bo, Liedberg Zhang, Hua Label-free, electrochemical detection of methicillin-resistant staphylococcus aureus DNA with reduced graphene oxide-modified electrodes |
| title | Label-free, electrochemical detection of methicillin-resistant staphylococcus aureus DNA with reduced graphene oxide-modified electrodes |
| title_full | Label-free, electrochemical detection of methicillin-resistant staphylococcus aureus DNA with reduced graphene oxide-modified electrodes |
| title_fullStr | Label-free, electrochemical detection of methicillin-resistant staphylococcus aureus DNA with reduced graphene oxide-modified electrodes |
| title_full_unstemmed | Label-free, electrochemical detection of methicillin-resistant staphylococcus aureus DNA with reduced graphene oxide-modified electrodes |
| title_short | Label-free, electrochemical detection of methicillin-resistant staphylococcus aureus DNA with reduced graphene oxide-modified electrodes |
| title_sort | label free electrochemical detection of methicillin resistant staphylococcus aureus dna with reduced graphene oxide modified electrodes |
| topic | DRNTU::Science::Medicine::Biosensors |
| url | https://hdl.handle.net/10356/79512 http://hdl.handle.net/10220/8333 |
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