Pathways of energy transfer in LHCII revealed by room-temperature 2D electronic spectroscopy
We present here the first room-temperature 2D electronic spectroscopy study of energy transfer in the plant light-harvesting complex II, LHCII. Two-dimensional electronic spectroscopy has been used to study energy transfer dynamics in LHCII trimers from the chlorophyll b Qy band to the chlorophyll a...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
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2014
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| Online Access: | https://hdl.handle.net/10356/79517 http://hdl.handle.net/10220/24530 |
| _version_ | 1824453558842425344 |
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| author | Wells, Kym L. Lambrev, Petar H. Zhang, Zhengyang Garab, Gyözö Tan, Howe-Siang |
| author2 | School of Physical and Mathematical Sciences |
| author_facet | School of Physical and Mathematical Sciences Wells, Kym L. Lambrev, Petar H. Zhang, Zhengyang Garab, Gyözö Tan, Howe-Siang |
| author_sort | Wells, Kym L. |
| collection | NTU |
| description | We present here the first room-temperature 2D electronic spectroscopy study of energy transfer in the plant light-harvesting complex II, LHCII. Two-dimensional electronic spectroscopy has been used to study energy transfer dynamics in LHCII trimers from the chlorophyll b Qy band to the chlorophyll a Qy band. Observing cross-peak regions corresponding to couplings between different excitonic states reveals partially resolved fine structure at the exciton level that cannot be isolated by pump–probe or linear spectroscopy measurements alone. Global analysis of the data has been performed to identify the pathways and time constants of energy transfer. The measured waiting time (Tw) dependent 2D spectra are found to be composed of 2D decay-associated spectra with three timescales (0.3 ps, 2.3 ps and >20 ps). Direct and multistep cascading pathways from the high-energy chlorophyll b states to the lowest-energy chlorophyll a states have been resolved occurring on time scales of hundreds of femtoseconds to picoseconds. |
| first_indexed | 2025-02-19T03:08:20Z |
| format | Journal Article |
| id | ntu-10356/79517 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2025-02-19T03:08:20Z |
| publishDate | 2014 |
| record_format | dspace |
| spelling | ntu-10356/795172023-02-28T19:29:25Z Pathways of energy transfer in LHCII revealed by room-temperature 2D electronic spectroscopy Wells, Kym L. Lambrev, Petar H. Zhang, Zhengyang Garab, Gyözö Tan, Howe-Siang School of Physical and Mathematical Sciences DRNTU::Science::Physics We present here the first room-temperature 2D electronic spectroscopy study of energy transfer in the plant light-harvesting complex II, LHCII. Two-dimensional electronic spectroscopy has been used to study energy transfer dynamics in LHCII trimers from the chlorophyll b Qy band to the chlorophyll a Qy band. Observing cross-peak regions corresponding to couplings between different excitonic states reveals partially resolved fine structure at the exciton level that cannot be isolated by pump–probe or linear spectroscopy measurements alone. Global analysis of the data has been performed to identify the pathways and time constants of energy transfer. The measured waiting time (Tw) dependent 2D spectra are found to be composed of 2D decay-associated spectra with three timescales (0.3 ps, 2.3 ps and >20 ps). Direct and multistep cascading pathways from the high-energy chlorophyll b states to the lowest-energy chlorophyll a states have been resolved occurring on time scales of hundreds of femtoseconds to picoseconds. ASTAR (Agency for Sci., Tech. and Research, S’pore) Published version 2014-12-26T01:10:42Z 2019-12-06T13:27:16Z 2014-12-26T01:10:42Z 2019-12-06T13:27:16Z 2014 2014 Journal Article Wells, K. L., Lambrev, P. H., Zhang, Z., Garab, G., & Tan, H.-S. (2014). Pathways of energy transfer in LHCII revealed by room-temperature 2D electronic spectroscopy. Physical chemistry chemical physics, 16(23), 11640-11646. https://hdl.handle.net/10356/79517 http://hdl.handle.net/10220/24530 10.1039/C4CP00876F en Physical chemistry chemical physics This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. 7 p. application/pdf |
| spellingShingle | DRNTU::Science::Physics Wells, Kym L. Lambrev, Petar H. Zhang, Zhengyang Garab, Gyözö Tan, Howe-Siang Pathways of energy transfer in LHCII revealed by room-temperature 2D electronic spectroscopy |
| title | Pathways of energy transfer in LHCII revealed by room-temperature 2D electronic spectroscopy |
| title_full | Pathways of energy transfer in LHCII revealed by room-temperature 2D electronic spectroscopy |
| title_fullStr | Pathways of energy transfer in LHCII revealed by room-temperature 2D electronic spectroscopy |
| title_full_unstemmed | Pathways of energy transfer in LHCII revealed by room-temperature 2D electronic spectroscopy |
| title_short | Pathways of energy transfer in LHCII revealed by room-temperature 2D electronic spectroscopy |
| title_sort | pathways of energy transfer in lhcii revealed by room temperature 2d electronic spectroscopy |
| topic | DRNTU::Science::Physics |
| url | https://hdl.handle.net/10356/79517 http://hdl.handle.net/10220/24530 |
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